Anti-proliferative effect of extremely low frequency electromagnetic field on preneoplastic lesions formation in the rat liver.

BMC cancer · 2010 · Benefit · Low evidence

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BenefitLow evidencePaper 4383

Exposure to 4.5 mT, 120 Hz ELF-EMF was associated with >50% decreases in the number and area of GGT-positive preneoplastic liver lesions and reduced GST-P expression versus sham exposure. Apoptosis measures (TUNEL-positive cells and cleaved caspase-3) were un…

Study snapshot

PublicationBMC cancer · 2010

Study typeanimal

Reported directionBenefit

Evidence ratingLow

Abstract

BACKGROUND: Recently, extremely low frequency electromagnetic fields (ELF-EMF) have been studied with great interest due to their possible effects on human health. In this study, we evaluated the effect of 4.5 mT-120 Hz ELF-EMF on the development of preneoplastic lesions in experimental hepatocarcinogenesis. METHODS: Male Fischer-344 rats were subjected to the modified resistant hepatocyte model and were exposed to 4.5 mT - 120 Hz ELF-EMF. The effects of the ELF-EMF on hepatocarcinogenesis, apoptosis, proliferation and cell cycle progression were evaluated by histochemical, TUNEL assay, caspase 3 levels, immunohistochemical and western blot analyses. RESULTS: The application of the ELF-EMF resulted in a decrease of more than 50% of the number and the area of gamma-glutamyl transpeptidase-positive preneoplastic lesions (P = 0.01 and P = 0.03, respectively) and glutathione S-transferase placental expression (P = 0.01). The number of TUNEL-positive cells and the cleaved caspase 3 levels were unaffected; however, the proliferating cell nuclear antigen, Ki-67, and cyclin D1 expression decreased significantly (P < or = 0.03), as compared to the sham-exposure group. CONCLUSION: The application of 4.5 mT-120 Hz ELF-EMF inhibits preneoplastic lesions chemically induced in the rat liver through the reduction of cell proliferation, without altering the apoptosis process.

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AI evidence extraction

Structured fields below were generated from the paper’s abstract and metadata to make the evidence easier to inspect. Automated extraction may be incomplete or incorrect; verify important details against the original paper.

Study typeanimal

Effect directionbenefit

PopulationMale Fischer-344 rats (modified resistant hepatocyte model of experimental hepatocarcinogenesis)

Sample sizeNot supplied

ExposureELF · 0.00012 MHz

Evidence strengthLowConfidence: 78% · Peer reviewed: yes

Main findings

Outcomes measured

Number and area of gamma-glutamyl transpeptidase (GGT)-positive preneoplastic lesions
Glutathione S-transferase placental (GST-P) expression
Apoptosis markers (TUNEL-positive cells, cleaved caspase-3 levels)
Cell proliferation/cell cycle markers (PCNA, Ki-67, cyclin D1)

Limitations

Exposure duration not reported in abstract
Sample size not reported in abstract
Animal model with chemically induced preneoplastic lesions; generalizability to humans not addressed in abstract

View raw extracted JSON

{
  "study_type": "animal",
  "exposure": {
    "band": "ELF",
    "source": null,
    "frequency_mhz": 0.00012,
    "sar_wkg": null,
    "duration": null
  },
  "population": "Male Fischer-344 rats (modified resistant hepatocyte model of experimental hepatocarcinogenesis)",
  "sample_size": null,
  "outcomes": [
    "Number and area of gamma-glutamyl transpeptidase (GGT)-positive preneoplastic lesions",
    "Glutathione S-transferase placental (GST-P) expression",
    "Apoptosis markers (TUNEL-positive cells, cleaved caspase-3 levels)",
    "Cell proliferation/cell cycle markers (PCNA, Ki-67, cyclin D1)"
  ],
  "main_findings": "Exposure to 4.5 mT, 120 Hz ELF-EMF was associated with >50% decreases in the number and area of GGT-positive preneoplastic liver lesions and reduced GST-P expression versus sham exposure. Apoptosis measures (TUNEL-positive cells and cleaved caspase-3) were unchanged, while proliferation/cell-cycle markers (PCNA, Ki-67, cyclin D1) decreased significantly.",
  "effect_direction": "benefit",
  "limitations": [
    "Exposure duration not reported in abstract",
    "Sample size not reported in abstract",
    "Animal model with chemically induced preneoplastic lesions; generalizability to humans not addressed in abstract"
  ],
  "evidence_strength": "low",
  "confidence": 0.78,
  "peer_reviewed_likely": "yes",
  "keywords": [
    "ELF-EMF",
    "extremely low frequency",
    "120 Hz",
    "4.5 mT",
    "rat",
    "liver",
    "hepatocarcinogenesis",
    "preneoplastic lesions",
    "GGT-positive lesions",
    "GST-P",
    "proliferation",
    "Ki-67",
    "PCNA",
    "cyclin D1",
    "apoptosis",
    "TUNEL",
    "caspase-3"
  ],
  "suggested_hubs": [
    {
      "slug": "elf-emf",
      "weight": 0.9,
      "reason": "Study evaluates 120 Hz extremely low frequency magnetic field exposure in an animal model."
    },
    {
      "slug": "cancer-animal-studies",
      "weight": 0.75,
      "reason": "Assesses effects on chemically induced preneoplastic liver lesions and proliferation markers in rats."
    }
  ]
}

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AI-extracted fields are generated from the abstract and metadata and may be incomplete or incorrect. This material is for informational purposes and is not medical advice.

Identifiers and source

DOI: 10.1186/1471-2407-10-159
PubMed: 20416104

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