Effect of Extremely Low Frequency Electromagnetic Fields (EMF) on Phospholipase Activity in the Cultured Cells.

The Korean journal of physiology & pharmacology : official journal of the Korean Physiological Society and the Korean Society of Pharmacology · 2010 · No effect · Low evidence

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No effectLow evidencePaper 4341

Exposure to 60 Hz EMF at 0.1 or 1 mT for 4 or 16 hours did not affect phospholipase A2, C, or D activities or arachidonic acid release in RAW 264.7 and RBL 2H3 cultured cells.

Study snapshot

PublicationThe Korean journal of physiology & pharmacology : official journal of the Korean Physiological Society and the Korean Society of Pharmacology · 2010

Study typein_vitro

Reported directionNo effect

Evidence ratingLow

Abstract

This study was conducted to investigate the effects of extremely low frequency electromagnetic fields (EMF) on signal pathway in plasma membrane of cultured cells (RAW 264.7 cells and RBL 2H3 cells), by measuring the activity of phospholipase A(2) (PLA(2)), phospholipase C (PLC) and phospholipase D (PLD). The cells were exposed to the EMF (60 Hz, 0.1 or 1 mT) for 4 or 16 h. The basal and 0.5 µM melittin-induced arachidonic acid release was not affected by EMF in both cells. In cell-free PLA(2) assay, we failed to observe the change of cPLA(2) and sPLA(2) activity. Also both PLC and PLD activities did not show any change in the two cell lines exposed to EMF. This study suggests that the exposure condition of EMF (60 Hz, 0.1 or 1 mT) which is 2.4 fold higher than the limit of occupational exposure does not induce phospholipases-associated signal pathway in RAW 264.7 cells and RBL 2H3 cells.

Machine Enhanced Logic

AI evidence extraction

Structured fields below were generated from the paper’s abstract and metadata to make the evidence easier to inspect. Automated extraction may be incomplete or incorrect; verify important details against the original paper.

Study typein_vitro

Effect directionno_effect

PopulationNot supplied

Sample sizeNot supplied

ExposureELF · 0.06 MHz · 4 or 16 h

Evidence strengthLowConfidence: 60% · Peer reviewed: yes

Main findings

Exposure to 60 Hz EMF at 0.1 or 1 mT for 4 or 16 hours did not affect phospholipase A2, C, or D activities or arachidonic acid release in RAW 264.7 and RBL 2H3 cultured cells.

Outcomes measured

phospholipase A2 activity
phospholipase C activity
phospholipase D activity
arachidonic acid release

Limitations

In vitro study, results may not translate to in vivo effects
Limited to two cell lines
Only specific phospholipase activities measured

View raw extracted JSON

{
  "study_type": "in_vitro",
  "exposure": {
    "band": "ELF",
    "source": null,
    "frequency_mhz": 0.06,
    "sar_wkg": null,
    "duration": "4 or 16 h"
  },
  "population": null,
  "sample_size": null,
  "outcomes": [
    "phospholipase A2 activity",
    "phospholipase C activity",
    "phospholipase D activity",
    "arachidonic acid release"
  ],
  "main_findings": "Exposure to 60 Hz EMF at 0.1 or 1 mT for 4 or 16 hours did not affect phospholipase A2, C, or D activities or arachidonic acid release in RAW 264.7 and RBL 2H3 cultured cells.",
  "effect_direction": "no_effect",
  "limitations": [
    "In vitro study, results may not translate to in vivo effects",
    "Limited to two cell lines",
    "Only specific phospholipase activities measured"
  ],
  "evidence_strength": "low",
  "confidence": 0.6,
  "peer_reviewed_likely": "yes",
  "keywords": [
    "extremely low frequency",
    "EMF",
    "phospholipase",
    "signal pathway",
    "cultured cells",
    "RAW 264.7",
    "RBL 2H3"
  ],
  "suggested_hubs": []
}

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AI-extracted fields are generated from the abstract and metadata and may be incomplete or incorrect. This material is for informational purposes and is not medical advice.

Identifiers and source

DOI: 10.4196/kjpp.2010.14.6.427
PubMed: 21311685

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