Nociceptin inhibits electric field stimulation-induced cholinergic constrictions in rat airways.

Acta pharmacologica Sinica · 2000 · Mixed · Low evidence

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MixedLow evidencePaper 3955

Study snapshot

PublicationActa pharmacologica Sinica · 2000

Study typeanimal

Reported directionMixed

Evidence ratingLow

Abstract

AIM: To study the effect of nociceptin (orphanin FQ), a newly discovered heptadecapeptide, on cholinergic constrictions in isolated trachea and bronchus of rat. METHODS: The electric field stimulation (EFS) induced a monophasic constriction, which was due to an activation of the cholinergic nerves. RESULTS: Nociceptin 0.001-0.1 mumol/L inhibited cholinergic constriction in a concentration-dependent manner. IC50 (95% of confidence limits) were 0.06 (0.04-0.08) mumol/L and 0.07 (0.05-0.1) mumol/L in tracheae and bronchi respectively. The constrictions inhibited by nociceptin 0.01 mumol/L in traheae and bronchi were (58 +/- 32)% and (60 +/- 26)% respectively compared with the control, in which nociceptin was not applied. After pretreatment with naloxone 0.1 mumol/L, the constrictions were (60 +/- 19)% and (54 +/- 20)% (P > 0.05 vs the above figures). However, the constrictions induced by exogenous acetylcholine were unaffected by nociceptin 0.01 mumol/L. kappa-Opioid receptor agonist, U-50488H (0.01-1 mumol/L) did not affect the EFS-induced cholinergic constrictions in rat airways. CONCLUSION: Nociceptin inhibits EFS-induced cholinergic constriction, which is not affected by naloxone in rat airways.

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AI evidence extraction

Structured fields below were generated from the paper’s abstract and metadata to make the evidence easier to inspect. Automated extraction may be incomplete or incorrect; verify important details against the original paper.

Study typeanimal

Effect directionmixed

PopulationIsolated trachea and bronchus of rat

Sample sizeNot supplied

ExposureNot supplied

Evidence strengthLowConfidence: 74% · Peer reviewed: yes

Main findings

Electric field stimulation induced a monophasic cholinergic constriction in isolated rat trachea and bronchus. Nociceptin (0.001–0.1 µmol/L) inhibited EFS-induced cholinergic constriction in a concentration-dependent manner; this inhibition was not significantly altered by naloxone pretreatment, and nociceptin did not affect constrictions induced by exogenous acetylcholine. U-50488H did not affect EFS-induced cholinergic constrictions.

Outcomes measured

Cholinergic constriction in isolated rat trachea and bronchus (EFS-induced)
Response to exogenous acetylcholine
Effect of naloxone pretreatment
Effect of kappa-opioid receptor agonist U-50488H

Limitations

No EFS parameters reported (e.g., frequency, field strength, pulse characteristics).
Sample size not reported in abstract.
Ex vivo isolated tissue preparation; generalizability to in vivo physiology not established in abstract.
Focus is pharmacologic modulation of EFS-evoked responses rather than environmental EMF exposure.

View raw extracted JSON

{
  "study_type": "animal",
  "exposure": {
    "band": null,
    "source": "electric field stimulation (EFS)",
    "frequency_mhz": null,
    "sar_wkg": null,
    "duration": null
  },
  "population": "Isolated trachea and bronchus of rat",
  "sample_size": null,
  "outcomes": [
    "Cholinergic constriction in isolated rat trachea and bronchus (EFS-induced)",
    "Response to exogenous acetylcholine",
    "Effect of naloxone pretreatment",
    "Effect of kappa-opioid receptor agonist U-50488H"
  ],
  "main_findings": "Electric field stimulation induced a monophasic cholinergic constriction in isolated rat trachea and bronchus. Nociceptin (0.001–0.1 µmol/L) inhibited EFS-induced cholinergic constriction in a concentration-dependent manner; this inhibition was not significantly altered by naloxone pretreatment, and nociceptin did not affect constrictions induced by exogenous acetylcholine. U-50488H did not affect EFS-induced cholinergic constrictions.",
  "effect_direction": "mixed",
  "limitations": [
    "No EFS parameters reported (e.g., frequency, field strength, pulse characteristics).",
    "Sample size not reported in abstract.",
    "Ex vivo isolated tissue preparation; generalizability to in vivo physiology not established in abstract.",
    "Focus is pharmacologic modulation of EFS-evoked responses rather than environmental EMF exposure."
  ],
  "evidence_strength": "low",
  "confidence": 0.74,
  "peer_reviewed_likely": "yes",
  "keywords": [
    "nociceptin",
    "orphanin FQ",
    "electric field stimulation",
    "EFS",
    "cholinergic nerves",
    "trachea",
    "bronchus",
    "rat",
    "airways",
    "acetylcholine",
    "naloxone",
    "U-50488H"
  ],
  "suggested_hubs": []
}

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AI-extracted fields are generated from the abstract and metadata and may be incomplete or incorrect. This material is for informational purposes and is not medical advice.

Identifiers and source

DOI: Not supplied
PubMed: 11501050

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