Dynamic changes of [Ca(2+)] (i) in cerebellar granule cells exposed to pulsed electric fields.

Science in China. Series C, Life sciences · 2000 · Mixed · Low evidence

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MixedLow evidencePaper 3923

Study snapshot

PublicationScience in China. Series C, Life sciences · 2000

Study typein_vitro

Reported directionMixed

Evidence ratingLow

Abstract

Intracellular free Ca(2+) concentration ([Ca(2+)](i)) in embryonic chick cerebellar granule cells loaded with fluo-3/AM and exposed to a single pulsed electric field was investigated using a confocal laser scanning microscope and fluorescent microscope equipped with CCD video imaging system. The results showed that [Ca(2+)](i) increased immediately and rose to the peak rapidly as the cells exposed to a single pulsed electric field. The amplitude and rate of the increases of [Ca(2+)](i) depend on the intensity of external electric field. In the presence of Ca(2+) chelant EGTA or Ca(2+) channels blocker La(3+) in the pulsation solutions, the increase of [Ca(2+)](i) was still observable. It was also observed that [Ca(2+)](i) of different intracellular areas in the cell elevated simultaneously while the peak of the increase of [Ca(2+)](i) in the poles of the cell preceded to the peak in its somata and recovered to a plateau within a short time.

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AI evidence extraction

Structured fields below were generated from the paper’s abstract and metadata to make the evidence easier to inspect. Automated extraction may be incomplete or incorrect; verify important details against the original paper.

Study typein_vitro

Effect directionmixed

PopulationEmbryonic chick cerebellar granule cells (cell culture)

Sample sizeNot supplied

Exposuresingle pulse

Evidence strengthLowConfidence: 74% · Peer reviewed: yes

Main findings

In embryonic chick cerebellar granule cells, a single pulsed electric field exposure was associated with an immediate increase in intracellular free Ca2+ concentration, reaching a rapid peak. The amplitude and rate of [Ca2+]i increase depended on the intensity of the external electric field. The [Ca2+]i increase remained observable in the presence of EGTA or the Ca2+ channel blocker La3+, and spatially the poles peaked before the somata and then recovered to a plateau within a short time.

Outcomes measured

Intracellular free Ca2+ concentration ([Ca2+]i) dynamics

Limitations

In vitro study in embryonic chick cells; generalizability to humans is unclear
Exposure parameters (e.g., pulse characteristics, field strength values) not provided in the abstract
Sample size and replication details not reported in the abstract

View raw extracted JSON

{
  "study_type": "in_vitro",
  "exposure": {
    "band": null,
    "source": "pulsed electric field",
    "frequency_mhz": null,
    "sar_wkg": null,
    "duration": "single pulse"
  },
  "population": "Embryonic chick cerebellar granule cells (cell culture)",
  "sample_size": null,
  "outcomes": [
    "Intracellular free Ca2+ concentration ([Ca2+]i) dynamics"
  ],
  "main_findings": "In embryonic chick cerebellar granule cells, a single pulsed electric field exposure was associated with an immediate increase in intracellular free Ca2+ concentration, reaching a rapid peak. The amplitude and rate of [Ca2+]i increase depended on the intensity of the external electric field. The [Ca2+]i increase remained observable in the presence of EGTA or the Ca2+ channel blocker La3+, and spatially the poles peaked before the somata and then recovered to a plateau within a short time.",
  "effect_direction": "mixed",
  "limitations": [
    "In vitro study in embryonic chick cells; generalizability to humans is unclear",
    "Exposure parameters (e.g., pulse characteristics, field strength values) not provided in the abstract",
    "Sample size and replication details not reported in the abstract"
  ],
  "evidence_strength": "low",
  "confidence": 0.74,
  "peer_reviewed_likely": "yes",
  "keywords": [
    "pulsed electric field",
    "cerebellar granule cells",
    "embryonic chick",
    "intracellular calcium",
    "[Ca2+]i",
    "fluo-3/AM",
    "confocal microscopy",
    "EGTA",
    "La3+"
  ],
  "suggested_hubs": []
}

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AI-extracted fields are generated from the abstract and metadata and may be incomplete or incorrect. This material is for informational purposes and is not medical advice.

Identifiers and source

DOI: 10.1007/bf02881720
PubMed: 18763118

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