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Electric field enhanced plasmid delivery to liver hepatocellular carcinomas.

Technology in cancer research & treatment · 2002 · Benefit · Insufficient evidence

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BenefitInsufficient evidencePaper 3830

Manipulating electrical parameters in an electroporation protocol produced different gene expression levels in liver hepatocellular carcinomas. Statistically significant gene expression levels differed by an order of magnitude between two electric field stren…

Study snapshot

PublicationTechnology in cancer research & treatment · 2002
Study typeother
Reported directionBenefit
Evidence ratingInsufficient

Abstract

Electric field enhanced molecular delivery for cancer research and treatment is a new technology that has demonstrated its effectiveness in clinical trials using bleomycin or cisplatin (Heller, R., Gilbert, R., Jaroszeski, M. J. Clinical applications of electrochemotherapy, Advanced Drug Delivery Reviews 35,119-129, 1999), as chemotherapeutic agents. The technology is being investigated in research applications for applicability as a method to enhance gene expression in a target tumor. Success is predicated on an appropriate effective electric field mediated delivery protocol that triggers significant appropriate gene expression duration and levels. An electric field mediated delivery protocol includes a set of conditions associated with the electric field, the electroporation signature, as well as parameters associated with the plasmid and the electric field applicator. Manipulation of the electrical parameters within the electroporation signature generates different gene expression levels in liver hepatocellular carcinomas. Statistically significant gene expression levels were obtained that differed by an order of magnitude when two different electric field strength and duration conditions were employed.

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AI evidence extraction

Structured fields below were generated from the paper’s abstract and metadata to make the evidence easier to inspect. Automated extraction may be incomplete or incorrect; verify important details against the original paper.

Study typeother
Effect directionbenefit
PopulationNot supplied
Sample sizeNot supplied
Exposuretwo different electric field strength and duration conditions (details not stated)
Evidence strengthInsufficientConfidence: 62% · Peer reviewed: yes

Main findings

Manipulating electrical parameters in an electroporation protocol produced different gene expression levels in liver hepatocellular carcinomas. Statistically significant gene expression levels differed by an order of magnitude between two electric field strength/duration conditions.

Outcomes measured

  • gene expression levels in liver hepatocellular carcinomas
  • plasmid (gene) delivery efficiency

Limitations

  • No quantitative electric field parameters (strength, pulse duration, waveform) provided in abstract
  • Population/model (human vs animal vs in vitro) not specified
  • Sample size not reported
  • No clinical outcomes reported; outcome limited to gene expression
View raw extracted JSON
{
  "study_type": "other",
  "exposure": {
    "band": null,
    "source": "electroporation (electric field mediated delivery)",
    "frequency_mhz": null,
    "sar_wkg": null,
    "duration": "two different electric field strength and duration conditions (details not stated)"
  },
  "population": null,
  "sample_size": null,
  "outcomes": [
    "gene expression levels in liver hepatocellular carcinomas",
    "plasmid (gene) delivery efficiency"
  ],
  "main_findings": "Manipulating electrical parameters in an electroporation protocol produced different gene expression levels in liver hepatocellular carcinomas. Statistically significant gene expression levels differed by an order of magnitude between two electric field strength/duration conditions.",
  "effect_direction": "benefit",
  "limitations": [
    "No quantitative electric field parameters (strength, pulse duration, waveform) provided in abstract",
    "Population/model (human vs animal vs in vitro) not specified",
    "Sample size not reported",
    "No clinical outcomes reported; outcome limited to gene expression"
  ],
  "evidence_strength": "insufficient",
  "confidence": 0.62,
  "peer_reviewed_likely": "yes",
  "keywords": [
    "electric field",
    "electroporation",
    "plasmid delivery",
    "gene expression",
    "liver",
    "hepatocellular carcinoma",
    "electrochemotherapy"
  ],
  "suggested_hubs": []
}
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AI-extracted fields are generated from the abstract and metadata and may be incomplete or incorrect. This material is for informational purposes and is not medical advice.

Identifiers and source

DOI: 10.1177/153303460200100506
PubMed: 12625761

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