[The effect of extremely low frequency magnetic fields on cytochrome oxidase subunit 1 mRNA transcription].

Zhonghua lao dong wei sheng zhi ye bing za zhi = Zhonghua laodong weisheng zhiyebing zazhi = Chinese journal of industrial hygiene and occupational diseases · 2002 · Harm · Low evidence

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HarmLow evidencePaper 3806

MF-1 cDNA sequence showed 100% homology with cytochrome oxidase subunit 1 (CO1). In HL-60, L1210 and CHL cells, exposure to 0.1 mT or 0.8 mT magnetic fields reduced CO1 transcription compared with controls after 20 minutes and after 24 hours (P<0.05).

Study snapshot

PublicationZhonghua lao dong wei sheng zhi ye bing za zhi = Zhonghua laodong weisheng zhiyebing zazhi = Chinese journal of industrial hygiene and occupational diseases · 2002

Study typein_vitro

Reported directionHarm

Evidence ratingLow

Abstract

OBJECTIVE: To clone and identify MF-1 gene which responded to extremely low frequency magnetic fields(ELF MF) in Daudi cells, and explore the response universality of MF-1 gene in several MF-sensitive cell lines, so as to provide experimental basis for revealing the mechanism of biological effects induced by magnetic field. METHODS: The DNA fragment of MF-1 was cloned and sequenced; the mRNA level of MF-1 gene were analysed in MF-sensitive cell lines(HL-60, L1210 and CHL) by Northern blot after these cells being treated with 0.1 mT and 0.8 mT MF for 20 minutes and 24 hours, respectively. RESULTS: The MF-1 cDNA sequence had 100% homology with cytochrome oxidase subunit 1 gene(CO1) by searching Gene Bank database; the transcription of CO1 in HL-60, L1210 and CHL cell lines which exposed to 0.1 mT and 0.8 mT MF for 20 minutes were significantly lower(0.38 +/- 0.12 and 0.37 +/- 0.04) than that of control(0.58 +/- 0.12) and so did for 24 hours exposure(0.46 +/- 0.09 and 0.45 +/- 0.09 vs 0.65 +/- 0.06) (P < 0.05). CONCLUSION: CO1 is a MF-responsive gene. Cytochrome oxidase activity may be affected through low level of CO1 transcription by magnetic fields, thus induce bioeffects in organisms.

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AI evidence extraction

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Study typein_vitro

Effect directionharm

PopulationCell lines (Daudi; MF-sensitive cell lines HL-60, L1210, CHL)

Sample sizeNot supplied

ExposureELF · 20 minutes and 24 hours

Evidence strengthLowConfidence: 78% · Peer reviewed: yes

Main findings

Outcomes measured

MF-1 gene identification (homology to cytochrome oxidase subunit 1, CO1)
CO1 mRNA transcription level (Northern blot)

Limitations

In vitro study; relevance to human health outcomes not assessed
Magnetic field frequency not reported in abstract
Sample size/replicates not reported
Only mRNA transcription measured; cytochrome oxidase activity inferred but not directly reported

View raw extracted JSON

{
  "study_type": "in_vitro",
  "exposure": {
    "band": "ELF",
    "source": null,
    "frequency_mhz": null,
    "sar_wkg": null,
    "duration": "20 minutes and 24 hours"
  },
  "population": "Cell lines (Daudi; MF-sensitive cell lines HL-60, L1210, CHL)",
  "sample_size": null,
  "outcomes": [
    "MF-1 gene identification (homology to cytochrome oxidase subunit 1, CO1)",
    "CO1 mRNA transcription level (Northern blot)"
  ],
  "main_findings": "MF-1 cDNA sequence showed 100% homology with cytochrome oxidase subunit 1 (CO1). In HL-60, L1210 and CHL cells, exposure to 0.1 mT or 0.8 mT magnetic fields reduced CO1 transcription compared with controls after 20 minutes and after 24 hours (P<0.05).",
  "effect_direction": "harm",
  "limitations": [
    "In vitro study; relevance to human health outcomes not assessed",
    "Magnetic field frequency not reported in abstract",
    "Sample size/replicates not reported",
    "Only mRNA transcription measured; cytochrome oxidase activity inferred but not directly reported"
  ],
  "evidence_strength": "low",
  "confidence": 0.78,
  "peer_reviewed_likely": "yes",
  "keywords": [
    "extremely low frequency",
    "magnetic field",
    "ELF MF",
    "cytochrome oxidase subunit 1",
    "CO1",
    "mRNA transcription",
    "Northern blot",
    "Daudi cells",
    "HL-60",
    "L1210",
    "CHL"
  ],
  "suggested_hubs": [
    {
      "slug": "occupational-exposure",
      "weight": 0.55,
      "reason": "Published in an industrial hygiene/occupational diseases journal and studies ELF magnetic field bioeffects relevant to occupational contexts."
    }
  ]
}

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AI-extracted fields are generated from the abstract and metadata and may be incomplete or incorrect. This material is for informational purposes and is not medical advice.

Identifiers and source

DOI: Not supplied
PubMed: 14694645

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