Extremely low frequency magnetic field enhances expression of a specific recombinant protein in bacterial host

Analytical biochemistry · 2022 · Mixed · Low evidence

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MixedLow evidencePaper 818

Continuous exposure to ELF magnetic fields with intensity 55 mT and frequencies 2.5–2.8 Hz significantly enhanced recombinant CD22 expression yield at all tested IPTG concentrations (0.25–1.25 mM). Magnetic field intensities other than 55 mT were reported to …

Study snapshot

PublicationAnalytical biochemistry · 2022

Study typein_vitro

Reported directionMixed

Evidence ratingLow

Abstract

Extremely low frequency magnetic field enhances expression of a specific recombinant protein in bacterial host Behnaz Rashidieh, Alireza Madjid Ansari, Mahdi Behdani, Behrad Darvishi, Mahdi Habibi-Anbouhi. Extremely low frequency magnetic field enhances expression of a specific recombinant protein in bacterial host. Anal Biochem. 2022 May 21;114745. doi: 10.1016/j.ab.2022.114745. Abstract Expression of proteins in bacterial host cells, particularly E.coli, has gained much attention in recent years. Low expression outcome is the main technical drawback associated with this procedure, further restricting its largescale application in industry. Therefore, application of new amendments or reformations are required before further proceedings. Extremely low frequency magnetic fields (ELF-MFs) have shown to significantly affect biological processes, including gene expression, in E.coli. In current study, we investigated whether application of ELF-MF could result in overexpression of proteins in E.coli or not. Cluster of differentiation-22 (CD22), as a model protein, was expressed in E.Coli Rosetta (DE3) under continuous exposure to ELF-MF after applying various concentrations of Isopropyl ß-d-1-thiogalactopyranoside (IPTG) (0.25-1.25 mM) as inducer. The strength and frequency of electromagnetic fields (EMFs) ranged between 15-100 mT and 2.5-20 Hz respectively. Interestingly, application of 55mT EMFs with frequencies ranging from 2.5 to 2.8 Hz significantly enhanced the yield of expression at all studied IPTG concentrations. Contrarily, EMFs with intensities other than 55 mT meaningfully declined protein expression at IPTG concentrations equal to 1 and 1.25 mM. In conclusion, application of specific range of ELF-MFs may be exploited as a new modification for enhancing heterologous expression of proteins in E.coli. pubmed.ncbi.nlm.nih.gov

Machine Enhanced Logic

AI evidence extraction

Structured fields below were generated from the paper’s abstract and metadata to make the evidence easier to inspect. Automated extraction may be incomplete or incorrect; verify important details against the original paper.

Study typein_vitro

Effect directionmixed

PopulationE. coli Rosetta (DE3) bacterial host cells expressing recombinant CD22

Sample sizeNot supplied

ExposureELF · continuous exposure (timing not further specified)

Evidence strengthLowConfidence: 74% · Peer reviewed: yes

Main findings

Outcomes measured

Recombinant protein (CD22) expression yield in E. coli under varying IPTG concentrations

Limitations

Sample size not reported in abstract
Exposure duration and detailed experimental conditions not fully described in abstract
Findings limited to an in vitro bacterial expression system and a single model recombinant protein (CD22)

View raw extracted JSON

{
  "study_type": "in_vitro",
  "exposure": {
    "band": "ELF",
    "source": "laboratory magnetic field exposure",
    "frequency_mhz": null,
    "sar_wkg": null,
    "duration": "continuous exposure (timing not further specified)"
  },
  "population": "E. coli Rosetta (DE3) bacterial host cells expressing recombinant CD22",
  "sample_size": null,
  "outcomes": [
    "Recombinant protein (CD22) expression yield in E. coli under varying IPTG concentrations"
  ],
  "main_findings": "Continuous exposure to ELF magnetic fields with intensity 55 mT and frequencies 2.5–2.8 Hz significantly enhanced recombinant CD22 expression yield at all tested IPTG concentrations (0.25–1.25 mM). Magnetic field intensities other than 55 mT were reported to meaningfully reduce protein expression at IPTG concentrations of 1 and 1.25 mM.",
  "effect_direction": "mixed",
  "limitations": [
    "Sample size not reported in abstract",
    "Exposure duration and detailed experimental conditions not fully described in abstract",
    "Findings limited to an in vitro bacterial expression system and a single model recombinant protein (CD22)"
  ],
  "evidence_strength": "low",
  "confidence": 0.74,
  "peer_reviewed_likely": "yes",
  "keywords": [
    "extremely low frequency",
    "ELF-MF",
    "magnetic field",
    "E. coli",
    "recombinant protein expression",
    "CD22",
    "IPTG",
    "gene expression",
    "protein yield"
  ],
  "suggested_hubs": [
    {
      "slug": "el-frequencies-elf",
      "weight": 0.9,
      "reason": "Study tests extremely low frequency magnetic fields (2.5–20 Hz) and varying magnetic flux densities (15–100 mT) in a laboratory setting."
    }
  ]
}

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AI-extracted fields are generated from the abstract and metadata and may be incomplete or incorrect. This material is for informational purposes and is not medical advice.

Identifiers and source

DOI: 10.1016/j.ab.2022.114745
PubMed: 35609685

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