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Aerodynamically assisted bio-jets: the development of a novel and direct non-electric field-driven methodology for engineering living organisms.

Biomedical materials (Bristol, England) · 2007 · Unclear · Insufficient evidence

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UnclearInsufficient evidencePaper 3390

The authors describe an aerodynamically assisted, non-electric field-driven jetting method that can form a near-monodispersed distribution of cell-bearing droplets under stable conditions. Bio-jetted primary organisms were assessed for short- and long-term vi…

Study snapshot

PublicationBiomedical materials (Bristol, England) · 2007
Study typeother
Reported directionUnclear
Evidence ratingInsufficient

Abstract

We recently demonstrated the ability to use electrified jets under stable conditions for the generation of cell-bearing droplets to the formation of composite threads which are biologically active. Our studies established that processed cells were viable over several generations post-jetting and -threading. These harmless and successful techniques for jet-based cell handling to deployment for precision deposition have great potential and widespread applications in bioengineering and biotechnology. Nonetheless, our investigations into 'bio-electrosprays' and 'cell electrospinning' have elucidated these jets having direct applicability in regenerative and therapeutic medicine to studies in developmental biology. For these very reasons, jet methodologies having the capability to safely handle living organisms for drop and placing are increasingly gaining the interests of life scientists. We now demonstrate yet another technique (a non-electric field-driven approach, previously never explored with jetting living cells), possessing the ability to directly handle the processing of primary living organisms by means of the flow of a cell suspension within a needle placed in a pressure chamber in the presence of an applied pressure difference. The technique we introduce here is referred to as 'aerodynamically assisted bio-jets/-jetting' which is driven completely by aerodynamic forces applied over an exit orifice by way of a differential pressure. Our investigations present an operational window in which stable jetting conditions are achieved for the formation of a near-monodispersed distribution of cell-bearing droplets and droplet residues. Finally, the aerodynamically bio-jetted living primary organisms are assessed (over both short and long time points) for cellular viability by means of FACScan, a flow cytometry technology which quantifies the percentage of living and dead cells. These advanced biophysical and bioengineering studies elucidate the emergence of a non-electric field-driven bio-jetting technology which now joins the cell jetting race.

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AI evidence extraction

Structured fields below were generated from the paper’s abstract and metadata to make the evidence easier to inspect. Automated extraction may be incomplete or incorrect; verify important details against the original paper.

Study typeother
Effect directionunclear
PopulationPrimary living organisms (cells in suspension)
Sample sizeNot supplied
ExposureNot supplied
Evidence strengthInsufficientConfidence: 66% · Peer reviewed: yes

Main findings

The authors describe an aerodynamically assisted, non-electric field-driven jetting method that can form a near-monodispersed distribution of cell-bearing droplets under stable conditions. Bio-jetted primary organisms were assessed for short- and long-term viability using flow cytometry (FACScan).

Outcomes measured

  • Cellular viability (percent living/dead cells) assessed by flow cytometry (FACScan)
  • Formation of near-monodispersed distribution of cell-bearing droplets/droplet residues
  • Stable jetting operational window

Limitations

  • No quantitative results for viability or droplet characteristics are provided in the abstract.
  • Sample size and specific organism/cell type are not specified.
  • No explicit comparison outcomes versus electric-field-driven methods are reported in the abstract.
View raw extracted JSON
{
  "study_type": "other",
  "exposure": {
    "band": null,
    "source": null,
    "frequency_mhz": null,
    "sar_wkg": null,
    "duration": null
  },
  "population": "Primary living organisms (cells in suspension)",
  "sample_size": null,
  "outcomes": [
    "Cellular viability (percent living/dead cells) assessed by flow cytometry (FACScan)",
    "Formation of near-monodispersed distribution of cell-bearing droplets/droplet residues",
    "Stable jetting operational window"
  ],
  "main_findings": "The authors describe an aerodynamically assisted, non-electric field-driven jetting method that can form a near-monodispersed distribution of cell-bearing droplets under stable conditions. Bio-jetted primary organisms were assessed for short- and long-term viability using flow cytometry (FACScan).",
  "effect_direction": "unclear",
  "limitations": [
    "No quantitative results for viability or droplet characteristics are provided in the abstract.",
    "Sample size and specific organism/cell type are not specified.",
    "No explicit comparison outcomes versus electric-field-driven methods are reported in the abstract."
  ],
  "evidence_strength": "insufficient",
  "confidence": 0.66,
  "peer_reviewed_likely": "yes",
  "keywords": [
    "bio-jets",
    "aerodynamically assisted jetting",
    "non-electric field-driven",
    "cell-bearing droplets",
    "cell handling",
    "bioengineering",
    "flow cytometry",
    "FACScan",
    "viability"
  ],
  "suggested_hubs": []
}
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AI-extracted fields are generated from the abstract and metadata and may be incomplete or incorrect. This material is for informational purposes and is not medical advice.

Identifiers and source

DOI: 10.1088/1748-6041/2/2/015
PubMed: 18458450

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