Effect of long-term exposure to mobile phone radiation on alpha-Int1 gene sequence of Candida albicans.

Saudi journal of biological sciences · 2016 · No effect · Low evidence

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No effectLow evidencePaper 2937

Candida albicans samples exposed to 900 MHz radiation for 10 to 490 hours showed no mutations in the alpha-Int1 gene sequence compared with unexposed controls under the described conditions.

Study snapshot

PublicationSaudi journal of biological sciences · 2016

Study typein_vitro

Reported directionNo effect

Evidence ratingLow

Abstract

Over the last decade, communication industries have witnessed a tremendous expansion, while, the biological effects of electromagnetic waves have not been fully elucidated. Current study aimed at evaluating the mutagenic effect of long-term exposure to 900-MHz radiation on alpha-Int1 gene sequences of Candida albicans. A standard 900 MHz radiation generator was used for radiation. 10 ml volumes from a stock suspension of C. albicans were transferred into 10 polystyrene tubes. Five tubes were exposed at 4 °C to a fixed magnitude of radiation with different time periods of 10, 70, 210, 350 and 490 h. The other 5 tubes were kept far enough from radiation. The samples underwent genomic DNA extraction. PCR amplification of alpha-Int1 gene sequence was done using one set of primers. PCR products were resolved using agarose gel electrophoresis and the nucleotide sequences were determined. All samples showed a clear electrophoretic band around 441 bp and further sequencing revealed the amplified DNA segments are related to alpha-Int1 gene of the yeast. No mutations in the gene were seen in radiation exposed samples. Long-term exposure of the yeast to mobile phone radiation under the above mentioned conditions had no mutagenic effect on alpha-Int1 gene sequence.

Machine Enhanced Logic

AI evidence extraction

Structured fields below were generated from the paper’s abstract and metadata to make the evidence easier to inspect. Automated extraction may be incomplete or incorrect; verify important details against the original paper.

Study typein_vitro

Effect directionno_effect

PopulationCandida albicans (yeast)

Sample size10

ExposureRF · 900 MHz · 10, 70, 210, 350, 490 h

Evidence strengthLowConfidence: 78% · Peer reviewed: yes

Main findings

Candida albicans samples exposed to 900 MHz radiation for 10 to 490 hours showed no mutations in the alpha-Int1 gene sequence compared with unexposed controls under the described conditions.

Outcomes measured

Mutations/sequence changes in alpha-Int1 gene (PCR amplification and nucleotide sequencing)

Limitations

Specific exposure magnitude not reported (only described as a fixed magnitude).
Specific dosimetry metrics (e.g., SAR) not reported.
In vitro yeast model; findings may not generalize to humans or other organisms.
Exposure conducted at 4°C; may not reflect typical biological conditions.

View raw extracted JSON

{
  "study_type": "in_vitro",
  "exposure": {
    "band": "RF",
    "source": "mobile phone",
    "frequency_mhz": 900,
    "sar_wkg": null,
    "duration": "10, 70, 210, 350, 490 h"
  },
  "population": "Candida albicans (yeast)",
  "sample_size": 10,
  "outcomes": [
    "Mutations/sequence changes in alpha-Int1 gene (PCR amplification and nucleotide sequencing)"
  ],
  "main_findings": "Candida albicans samples exposed to 900 MHz radiation for 10 to 490 hours showed no mutations in the alpha-Int1 gene sequence compared with unexposed controls under the described conditions.",
  "effect_direction": "no_effect",
  "limitations": [
    "Specific exposure magnitude not reported (only described as a fixed magnitude).",
    "Specific dosimetry metrics (e.g., SAR) not reported.",
    "In vitro yeast model; findings may not generalize to humans or other organisms.",
    "Exposure conducted at 4°C; may not reflect typical biological conditions."
  ],
  "evidence_strength": "low",
  "confidence": 0.78,
  "peer_reviewed_likely": "yes",
  "keywords": [
    "900 MHz",
    "radiofrequency",
    "mobile phone radiation",
    "Candida albicans",
    "alpha-Int1",
    "mutagenic effect",
    "PCR",
    "gene sequencing"
  ],
  "suggested_hubs": []
}

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AI-extracted fields are generated from the abstract and metadata and may be incomplete or incorrect. This material is for informational purposes and is not medical advice.

Identifiers and source

DOI: 10.1016/j.sjbs.2015.05.001
PubMed: 27081370

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