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Involvement of the p38 MAPK signaling cascade in stress response of RAW 264.7 macrophages

2017 · Harm · Low evidence

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HarmLow evidencePaper 2320

Exposure to extremely low-intensity centimeter microwaves stimulated production of several cytokines (IL-1, IL-6, TNF-α, INF-γ, IL-10), activated NF-κB and p38 MAPK signaling, and increased Hsp72 expression in RAW 264.7 macrophages. A p38 MAPK inhibitor abrog…

Study snapshot

Publicationmanual · 2017
Study typein_vitro
Reported directionHarm
Evidence ratingLow

Abstract

Involvement of the p38 MAPK signaling cascade in stress response of RAW 264.7 macrophages Novoselova EG, Glushkova OV, Khrenov MO, Parfenyuk SB, Lunin SM, Vinogradova EV, Novoselova TV, Fesenko EE. Involvement of the p38 MAPK signaling cascade in stress response of RAW 264.7 macrophages. Dokl Biol Sci. 2017 Sep;476(1):203-205. doi: 0.1134/S0012496617050015. Abstract The role of the p38 MAPK signaling cascade was studied in stress response of RAW 264.7 macrophages to extremely low-intensity centimeter microwaves. Irradiation stimulated production of a number of cytokines (IL- 1, IL-6, TNF-α, INF-γ and IL-10), as well as induced activation of the signaling cascades NF- κB and p38 MAPK, and enhanced expression of Hsp72 heat shock protein. In the presence of the cascade p38 MAPK inhibitor (p38 MAP kinase inhibitor XI), the stimulating effects of electromagnetic waves were abrogated either completely (for NF- κB and Hsp72) or partially (for p38 MAPK and cytokines). The results obtained are indicative of a high sensitivity of the signaling cascade p38 MAPK to the effect of low-intensity physical fields. ncbi.nlm.nih.gov

Machine Enhanced Logic

AI evidence extraction

Structured fields below were generated from the paper’s abstract and metadata to make the evidence easier to inspect. Automated extraction may be incomplete or incorrect; verify important details against the original paper.

Study typein_vitro
Effect directionharm
PopulationRAW 264.7 macrophages (cell line)
Sample sizeNot supplied
Exposuremicrowave
Evidence strengthLowConfidence: 74% · Peer reviewed: yes

Main findings

Exposure to extremely low-intensity centimeter microwaves stimulated production of several cytokines (IL-1, IL-6, TNF-α, INF-γ, IL-10), activated NF-κB and p38 MAPK signaling, and increased Hsp72 expression in RAW 264.7 macrophages. A p38 MAPK inhibitor abrogated the reported stimulating effects completely for NF-κB and Hsp72 and partially for p38 MAPK and cytokines.

Outcomes measured

  • Cytokine production (IL-1, IL-6, TNF-α, INF-γ, IL-10)
  • NF-κB signaling activation
  • p38 MAPK signaling activation
  • Hsp72 heat shock protein expression
  • Effect of p38 MAPK inhibitor (p38 MAP kinase inhibitor XI) on responses

Limitations

  • Frequency not reported
  • Exposure metrics (e.g., SAR, power density) not reported
  • Exposure duration not reported
  • In vitro model; generalizability to humans not addressed in abstract
  • Sample size and statistical details not reported
View raw extracted JSON
{
  "study_type": "in_vitro",
  "exposure": {
    "band": "microwave",
    "source": null,
    "frequency_mhz": null,
    "sar_wkg": null,
    "duration": null
  },
  "population": "RAW 264.7 macrophages (cell line)",
  "sample_size": null,
  "outcomes": [
    "Cytokine production (IL-1, IL-6, TNF-α, INF-γ, IL-10)",
    "NF-κB signaling activation",
    "p38 MAPK signaling activation",
    "Hsp72 heat shock protein expression",
    "Effect of p38 MAPK inhibitor (p38 MAP kinase inhibitor XI) on responses"
  ],
  "main_findings": "Exposure to extremely low-intensity centimeter microwaves stimulated production of several cytokines (IL-1, IL-6, TNF-α, INF-γ, IL-10), activated NF-κB and p38 MAPK signaling, and increased Hsp72 expression in RAW 264.7 macrophages. A p38 MAPK inhibitor abrogated the reported stimulating effects completely for NF-κB and Hsp72 and partially for p38 MAPK and cytokines.",
  "effect_direction": "harm",
  "limitations": [
    "Frequency not reported",
    "Exposure metrics (e.g., SAR, power density) not reported",
    "Exposure duration not reported",
    "In vitro model; generalizability to humans not addressed in abstract",
    "Sample size and statistical details not reported"
  ],
  "evidence_strength": "low",
  "confidence": 0.74,
  "peer_reviewed_likely": "yes",
  "keywords": [
    "centimeter microwaves",
    "extremely low-intensity",
    "RAW 264.7",
    "macrophages",
    "p38 MAPK",
    "NF-κB",
    "cytokines",
    "Hsp72",
    "stress response"
  ],
  "suggested_hubs": []
}
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AI-extracted fields are generated from the abstract and metadata and may be incomplete or incorrect. This material is for informational purposes and is not medical advice.

Identifiers and source

DOI: Not supplied
PubMed: Not supplied

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