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Human Fibroblasts In Vitro Exposed to 2.45 GHz Continuous and Pulsed Wave Signals: Evaluation of Biological Effects with a Multimethodological Approach

2020 · No effect · Low evidence

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No effectLow evidencePaper 2146

Human fibroblasts exposed for 2 h to 2.45 GHz continuous-wave and pulsed-wave signals at SAR 0.7 W/kg showed no significant biological effects at the cellular or molecular level under the evaluated conditions.

Study snapshot

Publicationmanual · 2020
Study typein_vitro
Reported directionNo effect
Evidence ratingLow

Abstract

Human Fibroblasts In Vitro Exposed to 2.45 GHz Continuous and Pulsed Wave Signals: Evaluation of Biological Effects with a Multimethodological Approach Elisa Regalbuto, Anna Anselmo, Stefania De Sanctis, Valeria Franchini, Florigio Lista, Monica Benvenuto, Roberto Bei, Laura Masuelli, Guglielmo D'Inzeo, Alessandra Paffi, Eugenio Trodella, Antonella Sgura. Human Fibroblasts In Vitro Exposed to 2.45 GHz Continuous and Pulsed Wave Signals: Evaluation of Biological Effects with a Multimethodological Approach. Int J Mol Sci. 2020 Sep 25;21(19):E7069. doi: 10.3390/ijms21197069. Abstract The increasing exposure to radiofrequency electromagnetic fields (RF-EMF), especially from wireless communication devices, raises questions about their possible adverse health effects. So far, several in vitro studies evaluating RF-EMF genotoxic and cytotoxic non-thermal effects have reported contradictory results that could be mainly due to inadequate experimental design and lack of well-characterized exposure systems and conditions. Moreover, a topic poorly investigated is related to signal modulation induced by electromagnetic fields. The aim of this study was to perform an analysis of the potential non-thermal biological effects induced by 2.45 GHz exposures through a characterized exposure system and a multimethodological approach. Human fibroblasts were exposed to continuous (CW) and pulsed (PW) signals for 2 h in a wire patch cell-based exposure system at the specific absorption rate (SAR) of 0.7 W/kg. The evaluation of the potential biological effects was carried out through a multimethodological approach, including classical biological markers (genotoxic, cell cycle, and ultrastructural) and the evaluation of gene expression profile through the powerful high-throughput next generation sequencing (NGS) RNA sequencing (RNA-seq) approach. Our results suggest that 2.45 GHz radiofrequency fields did not induce significant biological effects at a cellular or molecular level for the evaluated exposure parameters and conditions. mdpi.com

Machine Enhanced Logic

AI evidence extraction

Structured fields below were generated from the paper’s abstract and metadata to make the evidence easier to inspect. Automated extraction may be incomplete or incorrect; verify important details against the original paper.

Study typein_vitro
Effect directionno_effect
PopulationHuman fibroblasts (in vitro)
Sample sizeNot supplied
ExposureRF · 2450 MHz · 0.7 W/kg · 2 h
Evidence strengthLowConfidence: 78% · Peer reviewed: yes

Main findings

Human fibroblasts exposed for 2 h to 2.45 GHz continuous-wave and pulsed-wave signals at SAR 0.7 W/kg showed no significant biological effects at the cellular or molecular level under the evaluated conditions.

Outcomes measured

  • Genotoxicity
  • Cell cycle effects
  • Ultrastructural changes
  • Gene expression profile (RNA-seq/NGS)
  • Cytotoxicity (mentioned as a focus of prior literature; specific assays not detailed)

Limitations

  • Sample size not reported in the abstract
  • Only one exposure duration (2 h) and one SAR level (0.7 W/kg) described in the abstract
  • Findings limited to the specific in vitro model (human fibroblasts) and the characterized wire patch exposure system
  • Details of statistical methods and specific assay results are not provided in the abstract
View raw extracted JSON
{
  "study_type": "in_vitro",
  "exposure": {
    "band": "RF",
    "source": "wireless communication devices",
    "frequency_mhz": 2450,
    "sar_wkg": 0.7,
    "duration": "2 h"
  },
  "population": "Human fibroblasts (in vitro)",
  "sample_size": null,
  "outcomes": [
    "Genotoxicity",
    "Cell cycle effects",
    "Ultrastructural changes",
    "Gene expression profile (RNA-seq/NGS)",
    "Cytotoxicity (mentioned as a focus of prior literature; specific assays not detailed)"
  ],
  "main_findings": "Human fibroblasts exposed for 2 h to 2.45 GHz continuous-wave and pulsed-wave signals at SAR 0.7 W/kg showed no significant biological effects at the cellular or molecular level under the evaluated conditions.",
  "effect_direction": "no_effect",
  "limitations": [
    "Sample size not reported in the abstract",
    "Only one exposure duration (2 h) and one SAR level (0.7 W/kg) described in the abstract",
    "Findings limited to the specific in vitro model (human fibroblasts) and the characterized wire patch exposure system",
    "Details of statistical methods and specific assay results are not provided in the abstract"
  ],
  "evidence_strength": "low",
  "confidence": 0.78,
  "peer_reviewed_likely": "yes",
  "keywords": [
    "2.45 GHz",
    "radiofrequency",
    "RF-EMF",
    "continuous wave",
    "pulsed wave",
    "signal modulation",
    "SAR 0.7 W/kg",
    "human fibroblasts",
    "in vitro",
    "genotoxicity",
    "cell cycle",
    "ultrastructure",
    "RNA-seq",
    "NGS"
  ],
  "suggested_hubs": []
}
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AI-extracted fields are generated from the abstract and metadata and may be incomplete or incorrect. This material is for informational purposes and is not medical advice.

Identifiers and source

DOI: 10.3390/ijms21197069
PubMed: Not supplied

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