RF Safe

Effects of 1.8 GHz radiofrequency field on microstructure and bone metabolism of femur in mice

2018 · Mixed · Low evidence

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MixedLow evidencePaper 2124

Micro-CT and histology showed no significant changes in femur bone microstructure or related parameters in the RF-exposed group versus sham. Serum ALP and BALP activities increased (29.47% and 16.82%, respectively; P<0.05), while TRACP-5b did not differ signi…

Study snapshot

Publicationmanual · 2018
Study typeanimal
Reported directionMixed
Evidence ratingLow

Abstract

Effects of 1.8 GHz radiofrequency field on microstructure and bone metabolism of femur in mice Guo L, Zhang JP, Zhang KY, Wang HB, Wang H, An GZ, Zhou Y, Meng GL, Ding DR. Effects of 1.8 GHz radiofrequency field on microstructure and bone metabolism of femur in mice. Bioelectromagnetics. Published online Apr 30, 2018. Abstract To investigate the effects of 1.8 GHz radiofrequency (RF) field on bone microstructure and metabolism of femur in mice, C57BL/6 mice (male, age 4 weeks) were whole‐body exposed or sham exposed to 1.8 GHz RF field. Specific absorption rates of whole body and bone were approximately 2.70 and 1.14 W/kg (6 h/day for 28 days). After exposure, microstructure and morphology of femur were observed by microcomputed tomography (micro- CT), Hematoxylin and Eosin (HE) and Masson staining. Subsequently, bone parameters were calculated directly from the reconstructed images, including structure model index, bone mineral density, trabecular bone volume/total volume, connectivity density, trabecular number, trabecular thickness, and trabecular separation. Biomarkers that reflect bone metabolism, such as serum total alkaline phosphatase (ALP), bone-specific alkaline phosphatase (BALP), and tartrate-resistant acid phosphatase 5b (TRACP-5b), were determined by biochemical assay methods. Micro-CT and histology results showed that there was no significant change in bone microstructure and the above parameters in RF group, compared with sham group. The activity of serum ALP and BALP increased 29.47% and 16.82%, respectively, in RF group, compared with sham group (P < 0.05). In addition, there were no significant differences in the activity of serum TRACP-5b between RF group and sham group. In brief, under present experimental conditions, we did not find support for an effect of 1.8 GHz RF field on bone microstructure; however, it might promote metabolic function of osteoblasts in mice. doi.org

Machine Enhanced Logic

AI evidence extraction

Structured fields below were generated from the paper’s abstract and metadata to make the evidence easier to inspect. Automated extraction may be incomplete or incorrect; verify important details against the original paper.

Study typeanimal
Effect directionmixed
PopulationC57BL/6 mice (male, age 4 weeks)
Sample sizeNot supplied
ExposureRF · 1800 MHz · 2.7 W/kg · 6 h/day for 28 days
Evidence strengthLowConfidence: 78% · Peer reviewed: yes

Main findings

Micro-CT and histology showed no significant changes in femur bone microstructure or related parameters in the RF-exposed group versus sham. Serum ALP and BALP activities increased (29.47% and 16.82%, respectively; P<0.05), while TRACP-5b did not differ significantly between groups.

Outcomes measured

  • Femur bone microstructure and morphology (micro-CT, HE, Masson staining)
  • Bone microstructure parameters (structure model index, bone mineral density, trabecular bone volume/total volume, connectivity density, trabecular number, trabecular thickness, trabecular separation)
  • Serum biomarkers of bone metabolism (total alkaline phosphatase [ALP], bone-specific alkaline phosphatase [BALP], tartrate-resistant acid phosphatase 5b [TRACP-5b])

Limitations

  • Sample size not reported in provided abstract
  • Exposure source/modulation details not described beyond frequency and SAR
  • Short exposure duration (28 days) and single species/sex/age group
View raw extracted JSON
{
  "study_type": "animal",
  "exposure": {
    "band": "RF",
    "source": null,
    "frequency_mhz": 1800,
    "sar_wkg": 2.7,
    "duration": "6 h/day for 28 days"
  },
  "population": "C57BL/6 mice (male, age 4 weeks)",
  "sample_size": null,
  "outcomes": [
    "Femur bone microstructure and morphology (micro-CT, HE, Masson staining)",
    "Bone microstructure parameters (structure model index, bone mineral density, trabecular bone volume/total volume, connectivity density, trabecular number, trabecular thickness, trabecular separation)",
    "Serum biomarkers of bone metabolism (total alkaline phosphatase [ALP], bone-specific alkaline phosphatase [BALP], tartrate-resistant acid phosphatase 5b [TRACP-5b])"
  ],
  "main_findings": "Micro-CT and histology showed no significant changes in femur bone microstructure or related parameters in the RF-exposed group versus sham. Serum ALP and BALP activities increased (29.47% and 16.82%, respectively; P<0.05), while TRACP-5b did not differ significantly between groups.",
  "effect_direction": "mixed",
  "limitations": [
    "Sample size not reported in provided abstract",
    "Exposure source/modulation details not described beyond frequency and SAR",
    "Short exposure duration (28 days) and single species/sex/age group"
  ],
  "evidence_strength": "low",
  "confidence": 0.78,
  "peer_reviewed_likely": "yes",
  "keywords": [
    "1.8 GHz",
    "radiofrequency",
    "RF exposure",
    "whole-body exposure",
    "SAR",
    "mice",
    "femur",
    "bone microstructure",
    "micro-CT",
    "bone metabolism",
    "alkaline phosphatase",
    "osteoblast"
  ],
  "suggested_hubs": []
}
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AI-extracted fields are generated from the abstract and metadata and may be incomplete or incorrect. This material is for informational purposes and is not medical advice.

Identifiers and source

DOI: 10.1002/bem.22125
PubMed: Not supplied

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