Cellular stress & caspase-3 resulting from combined two-frequency signal in brains of Sprague-Dawley rats

2016 · Mixed · Low evidence

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MixedLow evidencePaper 2086

Study snapshot

Publicationmanual · 2016

Study typeanimal

Reported directionMixed

Evidence ratingLow

Abstract

Cellular stress & caspase-3 resulting from combined two-frequency signal in brains of Sprague-Dawley rats López-Furelos A, Leiro-Vidal JM, Salas-Sánchez AÁ, Ares-Pena FJ, López-Martín ME. Evidence of cellular stress and caspase-3 resulting from a combined two-frequency signal in the cerebrum and cerebellum of sprague- dawley rats. Oncotarget. 2016 Oct 4;7(40):64674-64689. doi: 10.18632/oncotarget.11753. Abstract Multiple simultaneous exposures to electromagnetic signals induced adjustments in mammal nervous systems. In this study, we investigated the non-thermal SAR (Specific Absorption Rate) in the cerebral or cerebellar hemispheres of rats exposed in vivo to combined electromagnetic field (EMF) signals at 900 and 2450 MHz.Forty rats divided into four groups of 10 were individually exposed or not exposed to radiation in a GTEM chamber for one or two hours. After radiation, we used the Chemiluminescent Enzyme-Linked Immunosorbent Assay (ChELISA) technique to measure cellular stress levels, indicated by the presence of heat shock proteins (HSP) 90 and 70, as well as caspase-3-dependent pre-apoptotic activity in left and right cerebral and cerebellar hemispheres of Sprague Dawley rats.Twenty-four hours after exposure to combined or single radiation, significant differences were evident in HSP 90 and 70 but not in caspase 3 levels between the hemispheres of the cerebral cortex at high SAR levels. In the cerebellar hemispheres, groups exposed to a single radiofrequency (RF) and high SAR showed significant differences in HSP 90, 70 and caspase-3 levels compared to control animals. The absorbed energy and/or biological effects of combined signals were not additive, suggesting that multiple signals act on nervous tissue by a different mechanism. Open access paper: ncbi.nlm.nih.gov

Machine Enhanced Logic

AI evidence extraction

Structured fields below were generated from the paper’s abstract and metadata to make the evidence easier to inspect. Automated extraction may be incomplete or incorrect; verify important details against the original paper.

Study typeanimal

Effect directionmixed

PopulationSprague-Dawley rats

Sample size40

ExposureRF · one or two hours

Evidence strengthLowConfidence: 74% · Peer reviewed: yes

Main findings

Rats were exposed in vivo to combined EMF signals at 900 and 2450 MHz (single or combined exposures) for one or two hours. Twenty-four hours after exposure, significant differences were observed in HSP90 and HSP70 (but not caspase-3) between hemispheres of the cerebral cortex at high SAR levels; in cerebellar hemispheres, single RF high-SAR exposure groups showed significant differences in HSP90, HSP70, and caspase-3 compared with controls. The authors report that effects of combined signals were not additive.

Outcomes measured

HSP90 (cellular stress)
HSP70 (cellular stress)
caspase-3-dependent pre-apoptotic activity

Limitations

SAR values not reported in the provided abstract (only described as non-thermal and including 'high SAR' conditions)
Exposure source/modulation details not described beyond frequencies and GTEM chamber
Outcome reporting emphasizes hemisphere differences and selected comparisons; direction/magnitude of changes not fully specified in abstract

View raw extracted JSON

{
  "study_type": "animal",
  "exposure": {
    "band": "RF",
    "source": null,
    "frequency_mhz": null,
    "sar_wkg": null,
    "duration": "one or two hours"
  },
  "population": "Sprague-Dawley rats",
  "sample_size": 40,
  "outcomes": [
    "HSP90 (cellular stress)",
    "HSP70 (cellular stress)",
    "caspase-3-dependent pre-apoptotic activity"
  ],
  "main_findings": "Rats were exposed in vivo to combined EMF signals at 900 and 2450 MHz (single or combined exposures) for one or two hours. Twenty-four hours after exposure, significant differences were observed in HSP90 and HSP70 (but not caspase-3) between hemispheres of the cerebral cortex at high SAR levels; in cerebellar hemispheres, single RF high-SAR exposure groups showed significant differences in HSP90, HSP70, and caspase-3 compared with controls. The authors report that effects of combined signals were not additive.",
  "effect_direction": "mixed",
  "limitations": [
    "SAR values not reported in the provided abstract (only described as non-thermal and including 'high SAR' conditions)",
    "Exposure source/modulation details not described beyond frequencies and GTEM chamber",
    "Outcome reporting emphasizes hemisphere differences and selected comparisons; direction/magnitude of changes not fully specified in abstract"
  ],
  "evidence_strength": "low",
  "confidence": 0.74,
  "peer_reviewed_likely": "yes",
  "keywords": [
    "radiofrequency",
    "EMF",
    "900 MHz",
    "2450 MHz",
    "GTEM chamber",
    "non-thermal SAR",
    "Sprague-Dawley rat",
    "brain",
    "cerebrum",
    "cerebellum",
    "HSP70",
    "HSP90",
    "caspase-3",
    "apoptosis",
    "cellular stress"
  ],
  "suggested_hubs": []
}

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AI-extracted fields are generated from the abstract and metadata and may be incomplete or incorrect. This material is for informational purposes and is not medical advice.

Identifiers and source

DOI: 10.18632/oncotarget.11753
PubMed: Not supplied

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