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Study on the effects of terahertz radiation on gene networks of Escherichia coli by means of fluorescent biosensors

2020 · Mixed · Low evidence

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MixedLow evidencePaper 1911

Three fluorescent biosensors in E. coli were developed and exposed to THz radiation from a high-intensity pulsed short-wave free electron laser and a low-intensity continuous long-wave IMPATT-diode-based device. The threshold and dynamics of fluorescence depe…

Study snapshot

Publicationmanual · 2020
Study typein_vitro
Reported directionMixed
Evidence ratingLow

Abstract

Study on the effects of terahertz radiation on gene networks of Escherichia coli by means of fluorescent biosensors Danil S Serdyukov, Tatiana N Goryachkovskaya, Irina A Mescheryakova , Svetlana V Bannikova, Sergei A Kuznetsov, Olga P Cherkasova, Vasiliy M Popik, Sergey E Peltek. Study on the effects of terahertz radiation on gene networks of Escherichia coli by means of fluorescent biosensors. Biomed Opt Express. 2020 Aug 25;11(9):5258-5273. doi: 10.1364/BOE.400432. Abstract Three novel fluorescent biosensors sensitive to terahertz (THz) radiation were developed via transformation of Escherichia coli (E. coli) cells with plasmids, in which a promotor of genes matA, safA, or chbB controls the expression of a fluorescent protein. The biosensors were exposed to THz radiation from two sources: a high- intensity pulsed short-wave free electron laser and a low-intensity continuous long-wave IMPATT-diode-based device. The threshold and dynamics of fluorescence were found to depend on radiation parameters and exposure time. Heat shock or chemical stress yielded the absence of fluorescence induction. The biosensors are evaluated to be suitable for studying influence of THz radiation on the activity of gene networks related with considered gene promoters. ncbi.nlm.nih.gov

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AI evidence extraction

Structured fields below were generated from the paper’s abstract and metadata to make the evidence easier to inspect. Automated extraction may be incomplete or incorrect; verify important details against the original paper.

Study typein_vitro
Effect directionmixed
PopulationEscherichia coli cells transformed with plasmids carrying matA, safA, or chbB promoters controlling fluorescent protein expression
Sample sizeNot supplied
ExposureTHz
Evidence strengthLowConfidence: 74% · Peer reviewed: yes

Main findings

Three fluorescent biosensors in E. coli were developed and exposed to THz radiation from a high-intensity pulsed short-wave free electron laser and a low-intensity continuous long-wave IMPATT-diode-based device. The threshold and dynamics of fluorescence depended on radiation parameters and exposure time, while heat shock or chemical stress did not induce fluorescence.

Outcomes measured

  • Fluorescence induction dynamics/threshold in THz-sensitive biosensors (matA, safA, chbB promoter-controlled reporters)
  • Comparison of fluorescence induction under THz exposure vs heat shock or chemical stress

Limitations

  • No THz frequency, power density, or dosimetry metrics reported in the abstract
  • Exposure duration not specified in the abstract
  • Sample size and replication not reported in the abstract
  • Outcomes are reporter fluorescence responses rather than direct measures of gene expression or broader biological endpoints
View raw extracted JSON
{
  "study_type": "in_vitro",
  "exposure": {
    "band": "THz",
    "source": "free electron laser; IMPATT-diode-based device",
    "frequency_mhz": null,
    "sar_wkg": null,
    "duration": null
  },
  "population": "Escherichia coli cells transformed with plasmids carrying matA, safA, or chbB promoters controlling fluorescent protein expression",
  "sample_size": null,
  "outcomes": [
    "Fluorescence induction dynamics/threshold in THz-sensitive biosensors (matA, safA, chbB promoter-controlled reporters)",
    "Comparison of fluorescence induction under THz exposure vs heat shock or chemical stress"
  ],
  "main_findings": "Three fluorescent biosensors in E. coli were developed and exposed to THz radiation from a high-intensity pulsed short-wave free electron laser and a low-intensity continuous long-wave IMPATT-diode-based device. The threshold and dynamics of fluorescence depended on radiation parameters and exposure time, while heat shock or chemical stress did not induce fluorescence.",
  "effect_direction": "mixed",
  "limitations": [
    "No THz frequency, power density, or dosimetry metrics reported in the abstract",
    "Exposure duration not specified in the abstract",
    "Sample size and replication not reported in the abstract",
    "Outcomes are reporter fluorescence responses rather than direct measures of gene expression or broader biological endpoints"
  ],
  "evidence_strength": "low",
  "confidence": 0.74,
  "peer_reviewed_likely": "yes",
  "keywords": [
    "terahertz",
    "THz radiation",
    "Escherichia coli",
    "fluorescent biosensors",
    "gene networks",
    "free electron laser",
    "IMPATT diode",
    "matA",
    "safA",
    "chbB"
  ],
  "suggested_hubs": []
}
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AI-extracted fields are generated from the abstract and metadata and may be incomplete or incorrect. This material is for informational purposes and is not medical advice.

Identifiers and source

DOI: 10.1364/boe.400432
PubMed: Not supplied

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