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Study of the effects of 3.1 THz radiation on the expression of recombinant red fluorescent protein (RFP) in E. coli

2020 · Benefit · Low evidence

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BenefitLow evidencePaper 1900

After 8 hours of 3.1 THz irradiation, bacteria from the irradiated area had higher plasmid copy number (3.8×), protein expression (2.7×), and fluorescence intensity (3.3×) compared with bacteria from the un-irradiated area.

Study snapshot

Publicationmanual · 2020
Study typein_vitro
Reported directionBenefit
Evidence ratingLow

Abstract

Study of the effects of 3.1 THz radiation on the expression of recombinant red fluorescent protein (RFP) in E. coli Jiping Zhao, Erling Hu, Sen Shang, Dai Wu, Peng Li, Peng Zhang, Dan Tan, Xiaoyun Lu. Study of the effects of 3.1 THz radiation on the expression of recombinant red fluorescent protein (RFP) in E. coli. Biomed Opt Express. 2020 Jun 22;11(7):3890-3899. doi: 10.1364/BOE.392838. Abstract In recent years, many studies have been conducted to investigate the non-thermal effects of THz radiation on different organisms, but further studies are needed to fully elucidate the effects, especially on the molecular level. In this study, we explored the effects of at 3.1 THz radiation on protein expression in Escherichia coli (E. coli) using red fluorescent protein as a reporter molecule. After 8 hours of continuous THz irradiation of bacteria on LB (Luria-Bertani) solid plates at an average power of 33 mW/cm2 and 10 Hz pulse repetition frequency, we found that the plasmid copy number, protein expression and fluorescence intensity of bacteria from the irradiated area were 3.8-, 2.7-, and 3.3 times higher than in bacteria from the un-irradiated area, respectively. These findings suggest that plasmid replication changed significantly in bacteria exposed to 3.1 THz radiation, resulting in increased protein expression as evidenced by increased fluorescence intensity of the RFP reporter. Open access paper: ncbi.nlm.nih.gov

Machine Enhanced Logic

AI evidence extraction

Structured fields below were generated from the paper’s abstract and metadata to make the evidence easier to inspect. Automated extraction may be incomplete or incorrect; verify important details against the original paper.

Study typein_vitro
Effect directionbenefit
PopulationEscherichia coli (E. coli) on LB solid plates expressing recombinant red fluorescent protein (RFP) reporter
Sample sizeNot supplied
ExposureTHz · 3.1e+06 MHz · 8 hours continuous irradiation; 10 Hz pulse repetition frequency; average power 33 mW/cm2
Evidence strengthLowConfidence: 78% · Peer reviewed: yes

Main findings

After 8 hours of 3.1 THz irradiation, bacteria from the irradiated area had higher plasmid copy number (3.8×), protein expression (2.7×), and fluorescence intensity (3.3×) compared with bacteria from the un-irradiated area.

Outcomes measured

  • plasmid copy number
  • protein expression
  • fluorescence intensity (RFP)

Limitations

  • Sample size not reported in abstract
  • Only one organism/model system (E. coli) and one reporter (RFP) described in abstract
  • Exposure characterization limited to frequency, average power density, and pulse repetition frequency; temperature/thermal control not described in abstract
View raw extracted JSON
{
  "study_type": "in_vitro",
  "exposure": {
    "band": "THz",
    "source": null,
    "frequency_mhz": 3100000,
    "sar_wkg": null,
    "duration": "8 hours continuous irradiation; 10 Hz pulse repetition frequency; average power 33 mW/cm2"
  },
  "population": "Escherichia coli (E. coli) on LB solid plates expressing recombinant red fluorescent protein (RFP) reporter",
  "sample_size": null,
  "outcomes": [
    "plasmid copy number",
    "protein expression",
    "fluorescence intensity (RFP)"
  ],
  "main_findings": "After 8 hours of 3.1 THz irradiation, bacteria from the irradiated area had higher plasmid copy number (3.8×), protein expression (2.7×), and fluorescence intensity (3.3×) compared with bacteria from the un-irradiated area.",
  "effect_direction": "benefit",
  "limitations": [
    "Sample size not reported in abstract",
    "Only one organism/model system (E. coli) and one reporter (RFP) described in abstract",
    "Exposure characterization limited to frequency, average power density, and pulse repetition frequency; temperature/thermal control not described in abstract"
  ],
  "evidence_strength": "low",
  "confidence": 0.78,
  "peer_reviewed_likely": "yes",
  "keywords": [
    "terahertz",
    "THz radiation",
    "3.1 THz",
    "non-thermal effects",
    "Escherichia coli",
    "E. coli",
    "plasmid copy number",
    "protein expression",
    "red fluorescent protein",
    "RFP",
    "fluorescence intensity"
  ],
  "suggested_hubs": []
}
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AI-extracted fields are generated from the abstract and metadata and may be incomplete or incorrect. This material is for informational purposes and is not medical advice.

Identifiers and source

DOI: 10.1364/boe.392838
PubMed: Not supplied

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