Effects of 50 Hz magnetic field-exposed cell culture medium on cellular functions in human amniotic epithelial cells

2019 · No effect · Low evidence

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No effectLow evidencePaper 1822

SPR signals in culture medium decreased under 50 Hz magnetic field exposure in a time- and dose-dependent manner and were reversible after exposure ended. Magnetic-field pre-exposed culture medium did not significantly change cell viability, intracellular rea…

Study snapshot

Publicationmanual · 2019

Study typein_vitro

Reported directionNo effect

Evidence ratingLow

Abstract

Effects of 50 Hz magnetic field-exposed cell culture medium on cellular functions in human amniotic epithelial cells Fei Y, Su L, Lou H, Zhao C, Wang Y, Chen G. The effects of 50 Hz magnetic field-exposed cell culture medium on cellular functions in FL cells. J Radiat Res. 2019 May 21. pii: rrz020. doi: 10.1093/jrr/rrz020. Abstract Although extremely low frequency magnetic fields (ELF-MFs) have been classified as a possible carcinogen for humans by the International Agency for Research on Cancer (IARC), their biological effects and underlying mechanisms are still unclear. Our previous study indicated that ELF-MF exposure influenced the relative permittivity of the saline solution, suggesting that the MF exposure altered physical properties of the solution. To explore the biophysical mechanism of ELF-MF-induced biological effects, this study examined the effects of 50 Hz sinusoidal MF at 0-4.0 mT on the permittivity of culture medium with phase-interrogation surface plasmon resonance (SPR) sensing. Then, the biological effects of MF pre-exposed culture medium on cell viability, the mitogen-activated protein kinase (MAPK) signaling pathways, oxidative stress, and genetic stabilities were analyzed using Cell Counting Kit-8, western blot, flow cytometry, γH2AX foci formation, and comet assay. The results showed that SPR signals were decreased under MF exposure in a time- and dose- dependent manner, and the decreased SPR signals were reversible when the exposure was drawn off. However, MF pre-exposed culture medium did not significantly change cell viability, intracellular reactive oxygen species level, activation of the MARK signaling pathways, or genetic stabilities in human amniotic epithelial cells (FL cells). In conclusion, our data suggest that the relative permittivity of culture medium was influenced by 50 Hz MF exposure, but this change did not affect the biological processes in FL cells. ncbi.nlm.nih.gov

Machine Enhanced Logic

AI evidence extraction

Structured fields below were generated from the paper’s abstract and metadata to make the evidence easier to inspect. Automated extraction may be incomplete or incorrect; verify important details against the original paper.

Study typein_vitro

Effect directionno_effect

PopulationHuman amniotic epithelial cells (FL cells)

Sample sizeNot supplied

ExposureELF · 5e-05 MHz

Evidence strengthLowConfidence: 74% · Peer reviewed: yes

Main findings

Outcomes measured

Permittivity-related changes in culture medium assessed by phase-interrogation surface plasmon resonance (SPR) signals
Cell viability (Cell Counting Kit-8)
MAPK signaling pathway activation (western blot)
Oxidative stress / intracellular reactive oxygen species (flow cytometry)
Genetic stability / DNA damage (γH2AX foci formation, comet assay)

Limitations

Exposure duration not specified in abstract
Sample size not reported in abstract
Only evaluates effects of pre-exposed medium (not direct cell exposure)
Single cell type/model (FL cells)

View raw extracted JSON

{
  "study_type": "in_vitro",
  "exposure": {
    "band": "ELF",
    "source": "cell culture medium pre-exposed to magnetic field",
    "frequency_mhz": 5e-05,
    "sar_wkg": null,
    "duration": null
  },
  "population": "Human amniotic epithelial cells (FL cells)",
  "sample_size": null,
  "outcomes": [
    "Permittivity-related changes in culture medium assessed by phase-interrogation surface plasmon resonance (SPR) signals",
    "Cell viability (Cell Counting Kit-8)",
    "MAPK signaling pathway activation (western blot)",
    "Oxidative stress / intracellular reactive oxygen species (flow cytometry)",
    "Genetic stability / DNA damage (γH2AX foci formation, comet assay)"
  ],
  "main_findings": "SPR signals in culture medium decreased under 50 Hz magnetic field exposure in a time- and dose-dependent manner and were reversible after exposure ended. Magnetic-field pre-exposed culture medium did not significantly change cell viability, intracellular reactive oxygen species levels, MAPK signaling pathway activation, or genetic stability measures in FL cells.",
  "effect_direction": "no_effect",
  "limitations": [
    "Exposure duration not specified in abstract",
    "Sample size not reported in abstract",
    "Only evaluates effects of pre-exposed medium (not direct cell exposure)",
    "Single cell type/model (FL cells)"
  ],
  "evidence_strength": "low",
  "confidence": 0.74,
  "peer_reviewed_likely": "yes",
  "keywords": [
    "extremely low frequency",
    "ELF-MF",
    "50 Hz",
    "magnetic field",
    "cell culture medium",
    "permittivity",
    "surface plasmon resonance",
    "SPR",
    "MAPK",
    "oxidative stress",
    "ROS",
    "γH2AX",
    "comet assay",
    "genetic stability",
    "human amniotic epithelial cells",
    "FL cells"
  ],
  "suggested_hubs": [
    {
      "slug": "occupational-exposure",
      "weight": 0.25,
      "reason": "Study involves 50 Hz extremely low frequency magnetic fields, relevant to common power-frequency exposure contexts."
    }
  ]
}

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AI-extracted fields are generated from the abstract and metadata and may be incomplete or incorrect. This material is for informational purposes and is not medical advice.

Identifiers and source

DOI: 10.1093/jrr/rrz020
PubMed: Not supplied

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