Untargeted metabolomics unveil alterations of biomembranes permeability in human HaCaT keratinocytes

2019 · Mixed · Low evidence

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MixedLow evidencePaper 1751

Study snapshot

Publicationmanual · 2019

Study typein_vitro

Reported directionMixed

Evidence ratingLow

Abstract

Untargeted metabolomics unveil alterations of biomembranes permeability in human HaCaT keratinocytes upon 60 GHz millimeter-wave exposure Le Pogam P, Le Page Y, Habauzit D, Doué M, Zhadobov M, Sauleau R, Le Dréan Y, Rondeau D. Untargeted metabolomics unveil alterations of biomembranes permeability in human HaCaT keratinocytes upon 60 GHz millimeter-wave exposure. Sci Rep. 2019 Jun 27;9(1):9343. doi: 10.1038/s41598-019-45662-6. Abstract A joint metabolomic and lipidomic workflow is used to account for a potential effect of millimeter waves (MMW) around 60 GHz on biological tissues. For this purpose, HaCaT human keratinocytes were exposed at 60.4 GHz with an incident power density of 20 mW/cm², this value corresponding to the upper local exposure limit for general public in the context of a wide scale deployment of MMW technologies and devices. After a 24h-exposure, endo- and extracellular extracts were recovered to be submitted to an integrative UPLC-Q- Exactive metabolomic and lipidomic workflow. R-XCMS data processing and subsequent statistical treatment led to emphasize a limited number of altered features in lipidomic sequences and in intracellular metabolomic analyses, whatever the ionization mode (i.e 0 to 6 dysregulated features). Conversely, important dysregulations could be reported in extracellular metabolomic profiles with 111 and 99 frames being altered upon MMW exposure in positive and negative polarities, respectively. This unexpected extent of modifications can hardly stem from the mild changes that could be reported throughout transcriptomics studies, leading us to hypothesize that MMW might alter the permeability of cell membranes, as reported elsewhere. Open access paper: nature.com

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AI evidence extraction

Structured fields below were generated from the paper’s abstract and metadata to make the evidence easier to inspect. Automated extraction may be incomplete or incorrect; verify important details against the original paper.

Study typein_vitro

Effect directionmixed

PopulationHuman HaCaT keratinocytes (cell line)

Sample sizeNot supplied

ExposuremmWave · 60400 MHz · 24h

Evidence strengthLowConfidence: 78% · Peer reviewed: yes

Main findings

HaCaT keratinocytes exposed at 60.4 GHz (incident power density 20 mW/cm²) for 24 h showed few altered features in lipidomic sequences and intracellular metabolomic analyses (0 to 6 dysregulated features). In contrast, extracellular metabolomic profiles showed many altered frames (111 in positive polarity and 99 in negative polarity) upon millimeter-wave exposure. The authors hypothesize these changes may relate to altered cell membrane permeability.

Outcomes measured

Metabolomic profiles (intracellular and extracellular)
Lipidomic profiles
Dysregulated metabolic/lipid features
Hypothesized cell membrane permeability alteration

Limitations

Sample size not reported in abstract
Mechanistic conclusion (membrane permeability) is presented as a hypothesis rather than directly demonstrated
No SAR reported (incident power density provided instead)

View raw extracted JSON

{
  "study_type": "in_vitro",
  "exposure": {
    "band": "mmWave",
    "source": null,
    "frequency_mhz": 60400,
    "sar_wkg": null,
    "duration": "24h"
  },
  "population": "Human HaCaT keratinocytes (cell line)",
  "sample_size": null,
  "outcomes": [
    "Metabolomic profiles (intracellular and extracellular)",
    "Lipidomic profiles",
    "Dysregulated metabolic/lipid features",
    "Hypothesized cell membrane permeability alteration"
  ],
  "main_findings": "HaCaT keratinocytes exposed at 60.4 GHz (incident power density 20 mW/cm²) for 24 h showed few altered features in lipidomic sequences and intracellular metabolomic analyses (0 to 6 dysregulated features). In contrast, extracellular metabolomic profiles showed many altered frames (111 in positive polarity and 99 in negative polarity) upon millimeter-wave exposure. The authors hypothesize these changes may relate to altered cell membrane permeability.",
  "effect_direction": "mixed",
  "limitations": [
    "Sample size not reported in abstract",
    "Mechanistic conclusion (membrane permeability) is presented as a hypothesis rather than directly demonstrated",
    "No SAR reported (incident power density provided instead)"
  ],
  "evidence_strength": "low",
  "confidence": 0.78,
  "peer_reviewed_likely": "yes",
  "keywords": [
    "millimeter waves",
    "60 GHz",
    "60.4 GHz",
    "HaCaT",
    "keratinocytes",
    "metabolomics",
    "lipidomics",
    "UPLC-Q-Exactive",
    "power density 20 mW/cm²",
    "cell membrane permeability"
  ],
  "suggested_hubs": [
    {
      "slug": "5g-policy",
      "weight": 0.55,
      "reason": "Uses 60 GHz millimeter-wave exposure and references public exposure limits in context of wide-scale deployment of MMW technologies/devices."
    }
  ]
}

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AI-extracted fields are generated from the abstract and metadata and may be incomplete or incorrect. This material is for informational purposes and is not medical advice.

Identifiers and source

DOI: 10.1038/s41598-019-45662-6
PubMed: Not supplied

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