Extremely low frequency magnetic field induces human neuronal differentiation through NMDA receptor activation

2019 · Benefit · Low evidence

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BenefitLow evidencePaper 1730

Human neural progenitor cells differentiated more efficiently under ELF magnetic field exposure in vitro, with increased neuronal markers, higher intracellular Ca2+ levels (as evidenced by c-fos expression), and more elongated mature neurites. These effects w…

Study snapshot

Publicationmanual · 2019

Study typein_vitro

Reported directionBenefit

Evidence ratingLow

Abstract

Extremely low frequency magnetic field induces human neuronal differentiation through NMDA receptor activation Özgün A, Marote A, Behie LA, Salgado A, Garipcan B. Extremely low frequency magnetic field induces human neuronal differentiation through NMDA receptor activation. J Neural Transm (Vienna). 2019 Jul 17. doi: 10.1007/s00702-019-02045-5. Abstract Magnetic fields with different frequency and intensity parameters exhibit a wide range of effects on different biological models. Extremely low frequency magnetic field (ELF MF) exposure is known to augment or even initiate neuronal differentiation in several in vitro and in vivo models. This effect holds potential for clinical translation into treatment of neurodegenerative conditions such as autism, Parkinson's disease and dementia by promoting neurogenesis, non-invasively. However, the lack of information on underlying mechanisms hinders further investigation into this phenomenon. Here, we examine involvement of glutamatergic Ca2+ channel, N- methyl-D-aspartate (NMDA) receptors in the process of human neuronal differentiation under ELF MF exposure. We show that human neural progenitor cells (hNPCs) differentiate more efficiently under ELF MF exposure in vitro, as demonstrated by the abundance of neuronal markers. Furthermore, they exhibit higher intracellular Ca2+ levels as evidenced by c-fos expression and more elongated mature neurites. We were able to neutralize these effects by blocking NMDA receptors with memantine. As a result, we hypothesize that the effects of ELF MF exposure on neuronal differentiation originate from the effects on NMDA receptors, which sequentially triggers Ca2+-dependent cascades that lead to differentiation. Our findings identify NMDA receptors as a new key player in this field that will aid further research in the pursuit of effect mechanisms of ELF MFs. ncbi.nlm.nih.gov

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AI evidence extraction

Structured fields below were generated from the paper’s abstract and metadata to make the evidence easier to inspect. Automated extraction may be incomplete or incorrect; verify important details against the original paper.

Study typein_vitro

Effect directionbenefit

PopulationHuman neural progenitor cells (hNPCs) in vitro

Sample sizeNot supplied

ExposureELF

Evidence strengthLowConfidence: 74% · Peer reviewed: yes

Main findings

Outcomes measured

Neuronal differentiation efficiency
Abundance of neuronal markers
Intracellular Ca2+ levels (c-fos expression)
Neurite elongation/maturation
Effect of NMDA receptor blockade (memantine) on ELF MF-associated changes

Limitations

No ELF magnetic field frequency, intensity, or exposure duration parameters reported in the abstract
In vitro model; clinical relevance not established in the abstract
Sample size not reported in the abstract

View raw extracted JSON

{
  "study_type": "in_vitro",
  "exposure": {
    "band": "ELF",
    "source": null,
    "frequency_mhz": null,
    "sar_wkg": null,
    "duration": null
  },
  "population": "Human neural progenitor cells (hNPCs) in vitro",
  "sample_size": null,
  "outcomes": [
    "Neuronal differentiation efficiency",
    "Abundance of neuronal markers",
    "Intracellular Ca2+ levels (c-fos expression)",
    "Neurite elongation/maturation",
    "Effect of NMDA receptor blockade (memantine) on ELF MF-associated changes"
  ],
  "main_findings": "Human neural progenitor cells differentiated more efficiently under ELF magnetic field exposure in vitro, with increased neuronal markers, higher intracellular Ca2+ levels (as evidenced by c-fos expression), and more elongated mature neurites. These effects were neutralized by blocking NMDA receptors with memantine.",
  "effect_direction": "benefit",
  "limitations": [
    "No ELF magnetic field frequency, intensity, or exposure duration parameters reported in the abstract",
    "In vitro model; clinical relevance not established in the abstract",
    "Sample size not reported in the abstract"
  ],
  "evidence_strength": "low",
  "confidence": 0.74,
  "peer_reviewed_likely": "yes",
  "keywords": [
    "extremely low frequency",
    "ELF magnetic field",
    "human neural progenitor cells",
    "neuronal differentiation",
    "NMDA receptor",
    "calcium",
    "c-fos",
    "memantine",
    "neurite outgrowth"
  ],
  "suggested_hubs": []
}

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AI-extracted fields are generated from the abstract and metadata and may be incomplete or incorrect. This material is for informational purposes and is not medical advice.

Identifiers and source

DOI: 10.1007/s00702-019-02045-5
PubMed: Not supplied

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