Mitochondrial DNA damage and oxidative damage in HL-60 cells exposed to 900MHz RF

2017 · Harm · Low evidence

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HarmLow evidencePaper 1332

HL-60 cells exposed to continuous wave 900 MHz RF at 120 μW/cm² for 4 h/day for 5 days showed increased ROS and 8-OHdG, and decreased mitochondrial transcription factor A, mtDNA polymerase gamma, mtDNA transcripts, mtDNA copy number, and ATP versus sham contr…

Study snapshot

Publicationmanual · 2017

Study typein_vitro

Reported directionHarm

Evidence ratingLow

Abstract

Mitochondrial DNA damage and oxidative damage in HL-60 cells exposed to 900MHz RF Sun Y, Zong L, Gao Z, Zhu S, Tong J, Cao Y. Mitochondrial DNA damage and oxidative damage in HL-60 cells exposed to 900MHz radiofrequency fields. Mutat Res. 2017 Mar 7;797-799:7-14. doi: 10.1016/j.mrfmmm.2017.03.001. Abstract HL-60 cells, derived from human promyelocytic leukemia, were exposed to continuous wave 900MHz radiofrequency fields (RF) at 120μW/cm2 power intensity for 4h/day for 5 consecutive days to examine whether such exposure is capable damaging the mitochondrial DNA (mtDNA) mediated through the production of reactive oxygen species (ROS). In addition, the effect of RF exposure was examined on 8-hydroxy-2'- dexoyguanosine (8-OHdG) which is a biomarker for oxidative damage and on the mitochondrial synthesis of adenosine triphosphate (ATP) which is the energy required for cellular functions. The results indicated a significant increase in ROS and significant decreases in mitochondrial transcription factor A, mtDNA polymerase gamma, mtDNA transcripts and mtDNA copy number in RF-exposed cells compared with those in sham-exposed control cells. In addition, there was a significant increase in 8-OHdG and a significant decrease in ATP in RF- exposed cells. The response in positive control cells exposed to gamma radiation (GR, which is also known to induce ROS) was similar to those in RF-exposed cells. Thus, the overall data indicated that RF exposure was capable of inducing mtDNA damage mediated through ROS pathway which also induced oxidative damage. Prior-treatment of RF- and GR-exposed the cells with melatonin, a well-known free radical scavenger, reversed the effects observed in RF-exposed cells. ncbi.nlm.nih.gov

Machine Enhanced Logic

AI evidence extraction

Structured fields below were generated from the paper’s abstract and metadata to make the evidence easier to inspect. Automated extraction may be incomplete or incorrect; verify important details against the original paper.

Study typein_vitro

Effect directionharm

PopulationHL-60 cells (human promyelocytic leukemia cell line)

Sample sizeNot supplied

ExposureRF · 900 MHz · 4 h/day for 5 consecutive days (continuous wave)

Evidence strengthLowConfidence: 78% · Peer reviewed: yes

Main findings

Outcomes measured

Reactive oxygen species (ROS)
Mitochondrial transcription factor A
mtDNA polymerase gamma
mtDNA transcripts
mtDNA copy number
8-hydroxy-2'-deoxyguanosine (8-OHdG)
Adenosine triphosphate (ATP)
Mitochondrial DNA (mtDNA) damage

Limitations

In vitro cell-line study (HL-60), not a human/animal study
Specific exposure condition (continuous wave 900 MHz at 120 μW/cm²; 4 h/day for 5 days) may not generalize to other RF parameters
Sample size and blinding/randomization details not reported in the abstract
SAR not reported

View raw extracted JSON

{
  "study_type": "in_vitro",
  "exposure": {
    "band": "RF",
    "source": null,
    "frequency_mhz": 900,
    "sar_wkg": null,
    "duration": "4 h/day for 5 consecutive days (continuous wave)"
  },
  "population": "HL-60 cells (human promyelocytic leukemia cell line)",
  "sample_size": null,
  "outcomes": [
    "Reactive oxygen species (ROS)",
    "Mitochondrial transcription factor A",
    "mtDNA polymerase gamma",
    "mtDNA transcripts",
    "mtDNA copy number",
    "8-hydroxy-2'-deoxyguanosine (8-OHdG)",
    "Adenosine triphosphate (ATP)",
    "Mitochondrial DNA (mtDNA) damage"
  ],
  "main_findings": "HL-60 cells exposed to continuous wave 900 MHz RF at 120 μW/cm² for 4 h/day for 5 days showed increased ROS and 8-OHdG, and decreased mitochondrial transcription factor A, mtDNA polymerase gamma, mtDNA transcripts, mtDNA copy number, and ATP versus sham controls. The abstract reports that melatonin pre-treatment reversed the observed effects in RF-exposed cells.",
  "effect_direction": "harm",
  "limitations": [
    "In vitro cell-line study (HL-60), not a human/animal study",
    "Specific exposure condition (continuous wave 900 MHz at 120 μW/cm²; 4 h/day for 5 days) may not generalize to other RF parameters",
    "Sample size and blinding/randomization details not reported in the abstract",
    "SAR not reported"
  ],
  "evidence_strength": "low",
  "confidence": 0.78,
  "peer_reviewed_likely": "yes",
  "keywords": [
    "900 MHz",
    "radiofrequency",
    "RF",
    "HL-60",
    "mitochondrial DNA",
    "mtDNA damage",
    "oxidative damage",
    "ROS",
    "8-OHdG",
    "ATP",
    "melatonin"
  ],
  "suggested_hubs": [
    {
      "slug": "oxidative-stress",
      "weight": 0.9,
      "reason": "Reports increased ROS and oxidative damage marker 8-OHdG after RF exposure."
    }
  ]
}

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AI-extracted fields are generated from the abstract and metadata and may be incomplete or incorrect. This material is for informational purposes and is not medical advice.

Identifiers and source

DOI: 10.1016/j.mrfmmm.2017.03.001
PubMed: Not supplied

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