Effects of Nonthermal Radiofrequency Stimulation on Neuronal Activity and Neural Circuit in Mice

Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2023 · Harm · Low evidence

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HarmLow evidencePaper 562

Study snapshot

PublicationAdvanced science (Weinheim, Baden-Wurttemberg, Germany) · 2023

Study typeanimal

Reported directionHarm

Evidence ratingLow

Abstract

Effects of Nonthermal Radiofrequency Stimulation on Neuronal Activity and Neural Circuit in Mice Hao Y, Liu W, Liu Y, Liu Y, Xu Z, Ye Y, Zhou H, Deng H, Zuo H, Yang H, Li Y. Effects of Nonthermal Radiofrequency Stimulation on Neuronal Activity and Neural Circuit in Mice. Adv Sci (Weinh). 2023 Feb 8:e2205988. doi: 10.1002/advs.202205988. Abstract Whether the nonthermal effects of radiofrequency radiation (RFR) exist and how nonthermal RFR acts on the nervous system are unknown. An animal model of spatial memory impairment is established by exposing mice to 2856-MHz RFR in the range of thermal noise (≤1 °C). Glutamate release in the dorsal hippocampus (dHPC) CA1 region is not significantly changed after radiofrequency exposure, whereas dopamine release is reduced. Importantly, RFR enhances glutamatergic CA1 pyramidal neuron calcium activity by nonthermal mechanisms, which recover to the basal level with RFR termination. Furthermore, suppressed dHPC dopamine release induced by radiofrequency exposure is due to decreased density of dopaminergic projections from the locus coeruleus to dHPC, and artificial activation of dopamine axon terminals or D1 receptors in dHPC CA1 improve memory damage in mice exposed to RFR. These findings indicate that nonthermal radiofrequency stimulation modulates ongoing neuronal activity and affects nervous system function at the neural circuit level. pubmed.ncbi.nlm.nih.gov

Machine Enhanced Logic

AI evidence extraction

Structured fields below were generated from the paper’s abstract and metadata to make the evidence easier to inspect. Automated extraction may be incomplete or incorrect; verify important details against the original paper.

Study typeanimal

Effect directionharm

PopulationMice

Sample sizeNot supplied

Exposuremicrowave · 2856 MHz

Evidence strengthLowConfidence: 78% · Peer reviewed: yes

Main findings

Mice exposed to 2856-MHz radiofrequency radiation under nonthermal conditions (≤1 °C) showed reduced dopamine release in the dorsal hippocampus CA1 region, while glutamate release was not significantly changed. Radiofrequency exposure enhanced glutamatergic CA1 pyramidal neuron calcium activity in a reversible manner (returning to baseline after exposure ended). The reduced hippocampal dopamine release was attributed to decreased density of dopaminergic projections from the locus coeruleus to the dorsal hippocampus, and artificial activation of dopamine terminals or D1 receptors in CA1 improved memory damage in exposed mice.

Outcomes measured

Spatial memory impairment
Glutamate release in dorsal hippocampus CA1
Dopamine release in dorsal hippocampus
CA1 pyramidal neuron calcium activity
Density of dopaminergic projections from locus coeruleus to dorsal hippocampus
Effect of activating dopamine axon terminals or D1 receptors on memory damage

Limitations

Sample size not reported in abstract
Exposure duration and other dosimetry details (e.g., SAR) not reported in abstract
Animal study; generalizability to humans not addressed in abstract

View raw extracted JSON

{
  "study_type": "animal",
  "exposure": {
    "band": "microwave",
    "source": null,
    "frequency_mhz": 2856,
    "sar_wkg": null,
    "duration": null
  },
  "population": "Mice",
  "sample_size": null,
  "outcomes": [
    "Spatial memory impairment",
    "Glutamate release in dorsal hippocampus CA1",
    "Dopamine release in dorsal hippocampus",
    "CA1 pyramidal neuron calcium activity",
    "Density of dopaminergic projections from locus coeruleus to dorsal hippocampus",
    "Effect of activating dopamine axon terminals or D1 receptors on memory damage"
  ],
  "main_findings": "Mice exposed to 2856-MHz radiofrequency radiation under nonthermal conditions (≤1 °C) showed reduced dopamine release in the dorsal hippocampus CA1 region, while glutamate release was not significantly changed. Radiofrequency exposure enhanced glutamatergic CA1 pyramidal neuron calcium activity in a reversible manner (returning to baseline after exposure ended). The reduced hippocampal dopamine release was attributed to decreased density of dopaminergic projections from the locus coeruleus to the dorsal hippocampus, and artificial activation of dopamine terminals or D1 receptors in CA1 improved memory damage in exposed mice.",
  "effect_direction": "harm",
  "limitations": [
    "Sample size not reported in abstract",
    "Exposure duration and other dosimetry details (e.g., SAR) not reported in abstract",
    "Animal study; generalizability to humans not addressed in abstract"
  ],
  "evidence_strength": "low",
  "confidence": 0.78,
  "peer_reviewed_likely": "yes",
  "keywords": [
    "radiofrequency radiation",
    "nonthermal",
    "2856 MHz",
    "microwave",
    "mice",
    "spatial memory",
    "hippocampus",
    "CA1",
    "dopamine",
    "glutamate",
    "calcium activity",
    "locus coeruleus",
    "neural circuit",
    "D1 receptor"
  ],
  "suggested_hubs": [
    {
      "slug": "animal-studies",
      "weight": 0.9,
      "reason": "Experimental radiofrequency exposure study in mice assessing neural activity and behavior."
    }
  ]
}

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AI-extracted fields are generated from the abstract and metadata and may be incomplete or incorrect. This material is for informational purposes and is not medical advice.

Identifiers and source

DOI: 10.1002/advs.202205988
PubMed: 36755196

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