Low-frequency electromagnetic fields influence the expression of calcium metabolism related proteins in leukocytic cell lines

Environmental toxicology and pharmacology · 2023 · Mixed · Low evidence

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MixedLow evidencePaper 361

Study snapshot

PublicationEnvironmental toxicology and pharmacology · 2023

Study typein_vitro

Reported directionMixed

Evidence ratingLow

Abstract

Low-frequency electromagnetic fields influence the expression of calcium metabolism related proteins in leukocytic cell lines Wójcik-Piotrowicz K, Kaszuba-Zwoińska J, Piszczek P, Nowak B, Guzdek P, Gil K, Rokita E. Low-frequency electromagnetic fields influence the expression of calcium metabolism related proteins in leukocytic cell lines. Environ Toxicol Pharmacol. 2023 Nov 18:104320. doi: 10.1016/j.etap.2023.104320. Abstract Our study aimed to verify the hypothesis concerning low-frequency magnetic fields (LF-MFs)-related changes in cell viability through the biomechanism(s) based on calcineurin (CaN)-mediated signaling pathways triggered via ROS-like molecules. For experiments, Mono Mac 6 and U937 leukocytic cell lines were chosen and exposed to various LF-MFs and/or puromycin (PMC). The protein expression level of key regulatory proteins of calcium metabolism was examined by Western Blot analysis. In turn, the reactive oxygen species (ROS) and cell viability parameters were evaluated by cytochrome C reduction assay and flow cytometry, respectively. The simultaneous action of applied MF and PMC influenced cell viability in a MF-dependent manner. The changes in cell viability were correlated with protein expression and ROS levels. It was verified experimentally that applied stress stimuli influence cell susceptibility to undergo cell death. Moreover, the evoked bioeffects might be recognized as specific to both types of leukocyte populations. pubmed.ncbi.nlm.nih.gov

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AI evidence extraction

Structured fields below were generated from the paper’s abstract and metadata to make the evidence easier to inspect. Automated extraction may be incomplete or incorrect; verify important details against the original paper.

Study typein_vitro

Effect directionmixed

PopulationMono Mac 6 and U937 leukocytic cell lines

Sample sizeNot supplied

ExposureELF

Evidence strengthLowConfidence: 74% · Peer reviewed: yes

Main findings

Mono Mac 6 and U937 leukocytic cell lines exposed to various low-frequency magnetic fields (LF-MFs) and/or puromycin showed MF-dependent changes in cell viability when MF and puromycin were applied simultaneously. Changes in viability were correlated with protein expression of calcium metabolism-related regulatory proteins and ROS levels, and bioeffects appeared specific to the leukocyte population.

Outcomes measured

Cell viability
Protein expression of key regulatory proteins of calcium metabolism (calcineurin-mediated signaling related)
Reactive oxygen species (ROS) levels

Limitations

In vitro cell-line study; generalizability to humans not stated
Exposure parameters (frequency, field strength, duration) not provided in the abstract
Sample size and replication details not provided in the abstract
Co-exposure with puromycin complicates attribution to LF-MF alone

View raw extracted JSON

{
  "study_type": "in_vitro",
  "exposure": {
    "band": "ELF",
    "source": "other",
    "frequency_mhz": null,
    "sar_wkg": null,
    "duration": null
  },
  "population": "Mono Mac 6 and U937 leukocytic cell lines",
  "sample_size": null,
  "outcomes": [
    "Cell viability",
    "Protein expression of key regulatory proteins of calcium metabolism (calcineurin-mediated signaling related)",
    "Reactive oxygen species (ROS) levels"
  ],
  "main_findings": "Mono Mac 6 and U937 leukocytic cell lines exposed to various low-frequency magnetic fields (LF-MFs) and/or puromycin showed MF-dependent changes in cell viability when MF and puromycin were applied simultaneously. Changes in viability were correlated with protein expression of calcium metabolism-related regulatory proteins and ROS levels, and bioeffects appeared specific to the leukocyte population.",
  "effect_direction": "mixed",
  "limitations": [
    "In vitro cell-line study; generalizability to humans not stated",
    "Exposure parameters (frequency, field strength, duration) not provided in the abstract",
    "Sample size and replication details not provided in the abstract",
    "Co-exposure with puromycin complicates attribution to LF-MF alone"
  ],
  "evidence_strength": "low",
  "confidence": 0.74,
  "peer_reviewed_likely": "yes",
  "keywords": [
    "low-frequency magnetic fields",
    "LF-MF",
    "ELF",
    "leukocytic cell lines",
    "Mono Mac 6",
    "U937",
    "calcineurin",
    "calcium metabolism",
    "ROS",
    "cell viability",
    "puromycin",
    "Western blot",
    "flow cytometry"
  ],
  "suggested_hubs": [
    {
      "slug": "elF-emf-mechanisms",
      "weight": 0.6,
      "reason": "Examines ELF/LF magnetic-field bioeffects and proposed mechanisms involving ROS and calcineurin-mediated signaling in cell lines."
    }
  ]
}

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AI-extracted fields are generated from the abstract and metadata and may be incomplete or incorrect. This material is for informational purposes and is not medical advice.

Identifiers and source

DOI: 10.1016/j.etap.2023.104320
PubMed: 37984675

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