RF Safe

Evaluation of genotoxic effects in human peripheral blood leukocytes following an acute in vitro exposure to 900 MHz radiofrequency fields

Bioelectromagnetics · 2005 · No effect · Low evidence

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No effectLow evidencePaper 6840

Human peripheral blood leukocytes were exposed in vitro to 900 MHz GSM radiofrequency radiation for 2 hours at SARs of 0.3 and 1 W/kg under controlled dosimetry and temperature conditions. No statistically significant differences were found between exposed an…

Study snapshot

PublicationBioelectromagnetics · 2005
Study typein_vitro
Reported directionNo effect
Evidence ratingLow

Abstract

Human peripheral blood leukocytes from healthy volunteers have been employed to investigate the induction of genotoxic effects following 2 h exposure to 900 MHz radiofrequency radiation. The GSM signal has been studied at specific absorption rates (SAR) of 0.3 and 1 W/kg. The exposures were carried out in a waveguide system under strictly controlled conditions of both dosimetry and temperature. The same temperature conditions (37.0 +/- 0.1 degrees C) were realized in a second waveguide, employed to perform sham exposures. The induction of DNA damage was evaluated in leukocytes by applying the alkaline single cell gel electrophoresis (SCGE)/comet assay, while structural chromosome aberrations and sister chromatid exchanges were evaluated in lymphocytes stimulated with phytohemagglutinin. Alterations in kinetics of cell proliferation were determined by calculating the mitotic index. Positive controls were also provided by using methyl methanesulfonate (MMS) for comet assay and mitomycin-C (MMC), for chromosome aberration, or sister chromatid exchange tests. No statistically significant differences were detected in exposed samples in comparison with sham exposed ones for all the parameters investigated. On the contrary, the positive controls gave a statistically significant increase in DNA damage in all cases, as expected. Thus the results obtained in our experimental conditions do not support the hypothesis that 900 MHz radiofrequency field exposure induces DNA damage in human peripheral blood leukocytes in this range of SAR.

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AI evidence extraction

Structured fields below were generated from the paper’s abstract and metadata to make the evidence easier to inspect. Automated extraction may be incomplete or incorrect; verify important details against the original paper.

Study typein_vitro
Effect directionno_effect
PopulationHuman peripheral blood leukocytes/lymphocytes from healthy volunteers
Sample sizeNot supplied
ExposureRF · 900 MHz · 1 W/kg · 2 h acute in vitro exposure
Evidence strengthLowConfidence: 95% · Peer reviewed: yes

Main findings

Human peripheral blood leukocytes were exposed in vitro to 900 MHz GSM radiofrequency radiation for 2 hours at SARs of 0.3 and 1 W/kg under controlled dosimetry and temperature conditions. No statistically significant differences were found between exposed and sham-exposed samples for DNA damage, chromosome aberrations, sister chromatid exchanges, or mitotic index.

Outcomes measured

  • DNA damage by alkaline single cell gel electrophoresis (comet assay)
  • structural chromosome aberrations
  • sister chromatid exchanges
  • mitotic index/cell proliferation kinetics

Limitations

  • In vitro study
  • Acute exposure only (2 hours)
  • Sample size not reported in the abstract
View raw extracted JSON
{
  "study_type": "in_vitro",
  "exposure": {
    "band": "RF",
    "source": "GSM signal",
    "frequency_mhz": 900,
    "sar_wkg": 1,
    "duration": "2 h acute in vitro exposure"
  },
  "population": "Human peripheral blood leukocytes/lymphocytes from healthy volunteers",
  "sample_size": null,
  "outcomes": [
    "DNA damage by alkaline single cell gel electrophoresis (comet assay)",
    "structural chromosome aberrations",
    "sister chromatid exchanges",
    "mitotic index/cell proliferation kinetics"
  ],
  "main_findings": "Human peripheral blood leukocytes were exposed in vitro to 900 MHz GSM radiofrequency radiation for 2 hours at SARs of 0.3 and 1 W/kg under controlled dosimetry and temperature conditions. No statistically significant differences were found between exposed and sham-exposed samples for DNA damage, chromosome aberrations, sister chromatid exchanges, or mitotic index.",
  "effect_direction": "no_effect",
  "limitations": [
    "In vitro study",
    "Acute exposure only (2 hours)",
    "Sample size not reported in the abstract"
  ],
  "evidence_strength": "low",
  "confidence": 0.95,
  "peer_reviewed_likely": "yes",
  "keywords": [
    "900 MHz",
    "GSM",
    "radiofrequency radiation",
    "SAR",
    "in vitro",
    "human peripheral blood leukocytes",
    "lymphocytes",
    "DNA damage",
    "comet assay",
    "chromosome aberrations",
    "sister chromatid exchanges",
    "mitotic index",
    "genotoxicity"
  ],
  "suggested_hubs": []
}
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AI-extracted fields are generated from the abstract and metadata and may be incomplete or incorrect. This material is for informational purposes and is not medical advice.

Identifiers and source

DOI: 10.1002/bem.20078
PubMed: 15832336

Read the original paper →

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