Effects of low-level light therapy on streptozotocin-induced diabetic kidney

J Photochem Photobiol B · 2010 · Benefit · Low evidence

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BenefitLow evidencePaper 6733

Study snapshot

PublicationJ Photochem Photobiol B · 2010

Study typeanimal

Reported directionBenefit

Evidence ratingLow

Abstract

Hyperglycemia causes oxidative damage in tissues prone to complications in diabetes. Low-level light therapy (LLLT) in the red to near infrared range (630-1000nm) has been shown to accelerate diabetic wound healing. To test the hypothesis that LLLT would attenuate oxidative renal damage in Type I diabetic rats, male Wistar rats were made diabetic with streptozotocin (50mg/kg, ip), and then exposed to 670nm light at a dose of 9J/cm(2) once per day for 14weeks. The activity and expression of catalase and the activity of Na K-ATPase increased in kidneys of light-treated diabetic rats, whereas the activity and expression of glutathione peroxidase and the expression of Na K-ATPase were unchanged. LLLT lowered the values of serum BUN, serum creatinine, and BUN/creatinine ratio. In addition, LLLT augmented the activity and expression of cytochrome c oxidase, a primary photoacceptor molecule in the mitochondrial respiratory chain, and reduced the formation of the DNA adduct 8-hydroxy-2'-deoxyguanosine in kidney. LLLT improved renal function and antioxidant defense capabilities in the kidney of Type I diabetic rats. Thus, 670nm LLLT may be broadly applicable to the amelioration of renal complications induced by diabetes that disrupt antioxidant defense mechanisms.

Machine Enhanced Logic

AI evidence extraction

Structured fields below were generated from the paper’s abstract and metadata to make the evidence easier to inspect. Automated extraction may be incomplete or incorrect; verify important details against the original paper.

Study typeanimal

Effect directionbenefit

PopulationMale Wistar rats with streptozotocin-induced Type I diabetes

Sample sizeNot supplied

Exposureoptical (red/near-infrared) · 9 J/cm^2 once per day for 14 weeks

Evidence strengthLowConfidence: 74% · Peer reviewed: yes

Main findings

Diabetic rats exposed to 670 nm light (9 J/cm^2 daily for 14 weeks) showed increased kidney catalase activity/expression and increased Na,K-ATPase activity, with no change reported for glutathione peroxidase activity/expression and Na,K-ATPase expression. Light therapy lowered serum BUN, serum creatinine, and the BUN/creatinine ratio, increased cytochrome c oxidase activity/expression, and reduced kidney 8-OHdG formation; authors conclude renal function and antioxidant defenses improved.

Outcomes measured

Kidney catalase activity and expression
Kidney Na,K-ATPase activity and expression
Kidney glutathione peroxidase activity and expression
Serum BUN
Serum creatinine
BUN/creatinine ratio
Cytochrome c oxidase activity and expression
Kidney 8-hydroxy-2'-deoxyguanosine (8-OHdG) DNA adduct formation
Renal function
Antioxidant defense in kidney

Limitations

Sample size not reported in provided abstract/metadata
Animal model (streptozotocin-induced diabetic rats) may not generalize to humans
Exposure parameters beyond wavelength, dose, and schedule (e.g., irradiance, device details) not provided in abstract

View raw extracted JSON

{
  "study_type": "animal",
  "exposure": {
    "band": "optical (red/near-infrared)",
    "source": "low-level light therapy (LLLT)",
    "frequency_mhz": null,
    "sar_wkg": null,
    "duration": "9 J/cm^2 once per day for 14 weeks"
  },
  "population": "Male Wistar rats with streptozotocin-induced Type I diabetes",
  "sample_size": null,
  "outcomes": [
    "Kidney catalase activity and expression",
    "Kidney Na,K-ATPase activity and expression",
    "Kidney glutathione peroxidase activity and expression",
    "Serum BUN",
    "Serum creatinine",
    "BUN/creatinine ratio",
    "Cytochrome c oxidase activity and expression",
    "Kidney 8-hydroxy-2'-deoxyguanosine (8-OHdG) DNA adduct formation",
    "Renal function",
    "Antioxidant defense in kidney"
  ],
  "main_findings": "Diabetic rats exposed to 670 nm light (9 J/cm^2 daily for 14 weeks) showed increased kidney catalase activity/expression and increased Na,K-ATPase activity, with no change reported for glutathione peroxidase activity/expression and Na,K-ATPase expression. Light therapy lowered serum BUN, serum creatinine, and the BUN/creatinine ratio, increased cytochrome c oxidase activity/expression, and reduced kidney 8-OHdG formation; authors conclude renal function and antioxidant defenses improved.",
  "effect_direction": "benefit",
  "limitations": [
    "Sample size not reported in provided abstract/metadata",
    "Animal model (streptozotocin-induced diabetic rats) may not generalize to humans",
    "Exposure parameters beyond wavelength, dose, and schedule (e.g., irradiance, device details) not provided in abstract"
  ],
  "evidence_strength": "low",
  "confidence": 0.74,
  "peer_reviewed_likely": "yes",
  "keywords": [
    "low-level light therapy",
    "LLLT",
    "670 nm",
    "near-infrared",
    "red light",
    "diabetes",
    "streptozotocin",
    "Type I diabetic rats",
    "kidney",
    "renal function",
    "oxidative damage",
    "catalase",
    "glutathione peroxidase",
    "Na,K-ATPase",
    "cytochrome c oxidase",
    "8-hydroxy-2'-deoxyguanosine",
    "8-OHdG"
  ],
  "suggested_hubs": []
}

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AI-extracted fields are generated from the abstract and metadata and may be incomplete or incorrect. This material is for informational purposes and is not medical advice.

Identifiers and source

DOI: 10.1016/j.jphotobiol.2010.03.002
PubMed: 20356759

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