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Biodegradable, pH-sensitive chitosan beads obtained under microwave radiation for advanced cell culture.

Colloids and surfaces. B, Biointerfaces · 2018 · Unclear · Insufficient evidence

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UnclearInsufficient evidencePaper 6627

Chitosan beads were synthesized under microwave radiation using glutamic acid/1,5-pentanodiol crosslinking agents, with optional modification by Tilia platyphyllos extract. The beads were reported as non-cytotoxic by MTT assay and supported proliferation in L…

Study snapshot

PublicationColloids and surfaces. B, Biointerfaces · 2018
Study typein_vitro
Reported directionUnclear
Evidence ratingInsufficient

Abstract

A new type of promising chitosan beads with advanced properties were obtained under microwave radiation according to Green Chemistry principles. Biomaterials were prepared using chitosan as raw material and glutamic acid/1,5-pentanodiol mixture as crosslinking agents. Additionally beads were modified with Tilia platyphyllos extract to enhance their antioxidant properties. Beads were investigated over their chemical structure by FT-IR analysis. Also their morphology has been investigated by SEM method. Additionally swelling capacity of the obtained hydrogels was determined. Lack of cytotoxicity has been confirmed by MTT assay. Proliferation studies were carried out on L929 mouse fibroblasts. Advanced properties of the obtained beads were investigated by studying pH sensitivity and antioxidant properties by DPPH method. Also susceptibility to degradation and biodegradation by Sturm Test method was evaluated. Results shows that proposed chitosan beads and their eco-friendly synthesis method can be applied in cell therapy and tissue engineering.

Machine Enhanced Logic

AI evidence extraction

Structured fields below were generated from the paper’s abstract and metadata to make the evidence easier to inspect. Automated extraction may be incomplete or incorrect; verify important details against the original paper.

Study typein_vitro
Effect directionunclear
PopulationNot supplied
Sample sizeNot supplied
ExposureNot supplied
Evidence strengthInsufficientConfidence: 66% · Peer reviewed: yes

Main findings

Chitosan beads were synthesized under microwave radiation using glutamic acid/1,5-pentanodiol crosslinking agents, with optional modification by Tilia platyphyllos extract. The beads were reported as non-cytotoxic by MTT assay and supported proliferation in L929 mouse fibroblasts, with pH-sensitive, antioxidant, and biodegradable properties.

Outcomes measured

  • chemical structure (FT-IR)
  • morphology (SEM)
  • swelling capacity
  • cytotoxicity (MTT assay)
  • cell proliferation (L929 mouse fibroblasts)
  • pH sensitivity
  • antioxidant activity (DPPH)
  • degradation/biodegradation (Sturm Test)

Limitations

  • Microwave exposure parameters (e.g., frequency, power, duration) are not reported in the abstract.
  • No quantitative results or effect sizes are provided in the abstract.
  • The study focuses on microwave-assisted synthesis rather than biological effects of EMF exposure.
View raw extracted JSON
{
  "study_type": "in_vitro",
  "exposure": {
    "band": null,
    "source": "microwave radiation (used for synthesis)",
    "frequency_mhz": null,
    "sar_wkg": null,
    "duration": null
  },
  "population": null,
  "sample_size": null,
  "outcomes": [
    "chemical structure (FT-IR)",
    "morphology (SEM)",
    "swelling capacity",
    "cytotoxicity (MTT assay)",
    "cell proliferation (L929 mouse fibroblasts)",
    "pH sensitivity",
    "antioxidant activity (DPPH)",
    "degradation/biodegradation (Sturm Test)"
  ],
  "main_findings": "Chitosan beads were synthesized under microwave radiation using glutamic acid/1,5-pentanodiol crosslinking agents, with optional modification by Tilia platyphyllos extract. The beads were reported as non-cytotoxic by MTT assay and supported proliferation in L929 mouse fibroblasts, with pH-sensitive, antioxidant, and biodegradable properties.",
  "effect_direction": "unclear",
  "limitations": [
    "Microwave exposure parameters (e.g., frequency, power, duration) are not reported in the abstract.",
    "No quantitative results or effect sizes are provided in the abstract.",
    "The study focuses on microwave-assisted synthesis rather than biological effects of EMF exposure."
  ],
  "evidence_strength": "insufficient",
  "confidence": 0.66,
  "peer_reviewed_likely": "yes",
  "keywords": [
    "chitosan beads",
    "microwave radiation",
    "green chemistry",
    "hydrogel",
    "pH-sensitive",
    "antioxidant",
    "DPPH",
    "biodegradation",
    "Sturm test",
    "MTT assay",
    "L929 fibroblasts",
    "cell culture",
    "tissue engineering"
  ],
  "suggested_hubs": []
}
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AI-extracted fields are generated from the abstract and metadata and may be incomplete or incorrect. This material is for informational purposes and is not medical advice.

Identifiers and source

DOI: 10.1016/j.colsurfb.2018.01.061
PubMed: 29413612

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