Calreticulin attenuated microwave radiation-induced human microvascular endothelial cell injury through promoting actin acetylation and polymerization.

Cell stress & chaperones · 2017 · Mixed · Low evidence

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MixedLow evidencePaper 6588

Study snapshot

PublicationCell stress & chaperones · 2017

Study typein_vitro

Reported directionMixed

Evidence ratingLow

Abstract

Recent work reveals that actin acetylation modification has been linked to different normal and disease processes and the effects associated with metabolic and environmental stressors. Herein, we highlight the effects of calreticulin on actin acetylation and cell injury induced by microwave radiation in human microvascular endothelial cell (HMEC). HMEC injury was induced by high-power microwave of different power density (10, 30, 60, 100 mW/cm, for 6 min) with or without exogenous recombinant calreticulin. The cell injury was assessed by lactate dehydrogenase (LDH) activity and Cell Counting Kit-8 in culture medium, migration ability, intercellular junction, and cytoskeleton staining in HMEC. Western blotting analysis was used to detected calreticulin expression in cytosol and nucleus and acetylation of globular actin (G-actin). We found that HMEC injury was induced by microwave radiation in a dose-dependent manner. Pretreatment HMEC with calreticulin suppressed microwave radiation-induced LDH leakage and increased cell viability and improved microwave radiation-induced decrease in migration, intercellular junction, and cytoskeleton. Meanwhile, pretreatment HMEC with exogenous calreticulin upregulated the histone acetyltransferase activity and the acetylation level of G-actin and increased the fibrous actin (F-actin)/G-actin ratio. We conclude that exogenous calreticulin protects HMEC against microwave radiation-induced injury through promoting actin acetylation and polymerization.

Machine Enhanced Logic

AI evidence extraction

Structured fields below were generated from the paper’s abstract and metadata to make the evidence easier to inspect. Automated extraction may be incomplete or incorrect; verify important details against the original paper.

Study typein_vitro

Effect directionmixed

PopulationHuman microvascular endothelial cells (HMEC)

Sample sizeNot supplied

Exposuremicrowave · 6 min

Evidence strengthLowConfidence: 78% · Peer reviewed: yes

Main findings

High-power microwave exposure (10, 30, 60, 100 mW/cm for 6 min) induced HMEC injury in a dose-dependent manner. Pretreatment with exogenous recombinant calreticulin reduced LDH leakage, increased cell viability, and improved microwave-associated decreases in migration, intercellular junctions, and cytoskeleton measures; it also increased histone acetyltransferase activity, G-actin acetylation, and the F-actin/G-actin ratio.

Outcomes measured

Cell injury (LDH leakage/activity)
Cell viability (Cell Counting Kit-8)
Cell migration ability
Intercellular junction integrity
Cytoskeleton staining/structure
Calreticulin expression in cytosol and nucleus (Western blot)
Histone acetyltransferase activity
G-actin acetylation level
F-actin/G-actin ratio

Limitations

In vitro cell model; findings may not generalize to humans or in vivo conditions
Microwave frequency not reported in abstract
Exposure characterization limited to power density and duration; SAR not reported
Sample size and replication details not provided in abstract

View raw extracted JSON

{
  "study_type": "in_vitro",
  "exposure": {
    "band": "microwave",
    "source": null,
    "frequency_mhz": null,
    "sar_wkg": null,
    "duration": "6 min"
  },
  "population": "Human microvascular endothelial cells (HMEC)",
  "sample_size": null,
  "outcomes": [
    "Cell injury (LDH leakage/activity)",
    "Cell viability (Cell Counting Kit-8)",
    "Cell migration ability",
    "Intercellular junction integrity",
    "Cytoskeleton staining/structure",
    "Calreticulin expression in cytosol and nucleus (Western blot)",
    "Histone acetyltransferase activity",
    "G-actin acetylation level",
    "F-actin/G-actin ratio"
  ],
  "main_findings": "High-power microwave exposure (10, 30, 60, 100 mW/cm for 6 min) induced HMEC injury in a dose-dependent manner. Pretreatment with exogenous recombinant calreticulin reduced LDH leakage, increased cell viability, and improved microwave-associated decreases in migration, intercellular junctions, and cytoskeleton measures; it also increased histone acetyltransferase activity, G-actin acetylation, and the F-actin/G-actin ratio.",
  "effect_direction": "mixed",
  "limitations": [
    "In vitro cell model; findings may not generalize to humans or in vivo conditions",
    "Microwave frequency not reported in abstract",
    "Exposure characterization limited to power density and duration; SAR not reported",
    "Sample size and replication details not provided in abstract"
  ],
  "evidence_strength": "low",
  "confidence": 0.78,
  "peer_reviewed_likely": "yes",
  "keywords": [
    "microwave radiation",
    "human microvascular endothelial cells",
    "endothelial injury",
    "calreticulin",
    "actin acetylation",
    "G-actin",
    "F-actin",
    "histone acetyltransferase",
    "LDH",
    "cell viability",
    "migration",
    "intercellular junctions",
    "cytoskeleton"
  ],
  "suggested_hubs": []
}

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AI-extracted fields are generated from the abstract and metadata and may be incomplete or incorrect. This material is for informational purposes and is not medical advice.

Identifiers and source

DOI: 10.1007/s12192-016-0745-x
PubMed: 27815707

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