RF Safe

The Effects of Melatonin on Oxidative Stress Parameters and DNA Fragmentation in Testicular Tissue of Rats Exposed to Microwave Radiation.

Advances in clinical and experimental medicine : official organ Wroclaw Medical University · 2015 · Mixed · Low evidence

← Research stream
MixedLow evidencePaper 6570

In rats exposed to microwaves, melatonin (2 mg/kg i.p. daily) prevented increases in malondialdehyde after 20 days and reversed microwave-related changes in xanthine oxidase (after 40 days) and acid-DNase activity (after 20 days). Melatonin did not change pro…

Study snapshot

PublicationAdvances in clinical and experimental medicine : official organ Wroclaw Medical University · 2015
Study typeanimal
Reported directionMixed
Evidence ratingLow

Abstract

BACKGROUND: Microwaves from mobile phones are one of the environmental toxicants that are capable of compromising male fertility by inducing oxidative stress and apoptosis in the testes. Melatonin is a lipophilic tryptophan indole amine and a potent antioxidant. OBJECTIVES: The aim of the study was to evaluate the effect of melatonin treatment on oxidative stress parameters and DNA fragmentation in the testicular tissue of rats exposed to microwave radiation (4 h/day). MATERIAL AND METHODS: Adult Wistar rats were divided in 4 groups: I--treated with saline; II--treated with melatonin; III--exposed to microwaves; IV--exposed to microwaves and treated with melatonin. The melatonin (2 mg/kg ip) was administered daily. The animals were sacrificed after 20, 40 and 60 days. RESULTS: Melatonin treatment prevented previously registered increases in malondialdehyde after only 20 days. Furthermore, it reversed the effects of microwave exposure on xanthine oxidase (after 40 days) and acid-DNase activity (after 20 days). However, neither protein carbonyl content nor catalase and alkaline Dnase activity were changed due to melatonin treatment. CONCLUSIONS: Melatonin exerts potent antioxidant effects in the testes of rats exposed to microwaves by decreasing the intensity of oxidative stress; it also reduces DNA fragmentation.

Machine Enhanced Logic

AI evidence extraction

Structured fields below were generated from the paper’s abstract and metadata to make the evidence easier to inspect. Automated extraction may be incomplete or incorrect; verify important details against the original paper.

Study typeanimal
Effect directionmixed
PopulationAdult Wistar rats
Sample sizeNot supplied
Exposuremicrowave · 4 h/day; sacrificed after 20, 40 and 60 days
Evidence strengthLowConfidence: 74% · Peer reviewed: yes

Main findings

In rats exposed to microwaves, melatonin (2 mg/kg i.p. daily) prevented increases in malondialdehyde after 20 days and reversed microwave-related changes in xanthine oxidase (after 40 days) and acid-DNase activity (after 20 days). Melatonin did not change protein carbonyl content, catalase, or alkaline DNase activity. The authors conclude melatonin decreases oxidative stress intensity and reduces DNA fragmentation in testes of microwave-exposed rats.

Outcomes measured

  • Oxidative stress parameters in testicular tissue (malondialdehyde, xanthine oxidase, protein carbonyl content, catalase activity)
  • DNA fragmentation / DNase activity (acid-DNase, alkaline DNase)

Limitations

  • Frequency and SAR/exposure intensity not reported in abstract
  • Sample size not reported in abstract
  • Outcomes are biochemical markers in rats; fertility outcomes not reported in abstract
View raw extracted JSON
{
  "study_type": "animal",
  "exposure": {
    "band": "microwave",
    "source": "mobile phone",
    "frequency_mhz": null,
    "sar_wkg": null,
    "duration": "4 h/day; sacrificed after 20, 40 and 60 days"
  },
  "population": "Adult Wistar rats",
  "sample_size": null,
  "outcomes": [
    "Oxidative stress parameters in testicular tissue (malondialdehyde, xanthine oxidase, protein carbonyl content, catalase activity)",
    "DNA fragmentation / DNase activity (acid-DNase, alkaline DNase)"
  ],
  "main_findings": "In rats exposed to microwaves, melatonin (2 mg/kg i.p. daily) prevented increases in malondialdehyde after 20 days and reversed microwave-related changes in xanthine oxidase (after 40 days) and acid-DNase activity (after 20 days). Melatonin did not change protein carbonyl content, catalase, or alkaline DNase activity. The authors conclude melatonin decreases oxidative stress intensity and reduces DNA fragmentation in testes of microwave-exposed rats.",
  "effect_direction": "mixed",
  "limitations": [
    "Frequency and SAR/exposure intensity not reported in abstract",
    "Sample size not reported in abstract",
    "Outcomes are biochemical markers in rats; fertility outcomes not reported in abstract"
  ],
  "evidence_strength": "low",
  "confidence": 0.74,
  "peer_reviewed_likely": "yes",
  "keywords": [
    "microwave radiation",
    "mobile phones",
    "melatonin",
    "oxidative stress",
    "testis",
    "DNA fragmentation",
    "malondialdehyde",
    "xanthine oxidase",
    "DNase",
    "Wistar rats"
  ],
  "suggested_hubs": [
    {
      "slug": "melatonin-antioxidants",
      "weight": 0.78,
      "reason": "Study tests melatonin as an antioxidant intervention against microwave-induced oxidative stress/DNA fragmentation in rat testes."
    }
  ]
}
AI can be wrong. Always verify against the paper.

AI-extracted fields are generated from the abstract and metadata and may be incomplete or incorrect. This material is for informational purposes and is not medical advice.

Identifiers and source

DOI: 10.17219/acem/43888
PubMed: 26467130

Read the original paper →

Related papers

← Back to the Research Library