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The effect of melatonin on the liver of rats exposed to microwave radiation.

Bratislavske lekarske listy · 2015 · Mixed · Low evidence

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MixedLow evidencePaper 6581

Microwave exposure (900 MHz) increased malondialdehyde (after 40 days), protein carbonyl content (after 20 days), catalase and xanthine oxidase activity (after 40 days), and deoxyribonuclease I activity (after 60 days), while deoxyribonuclease II activity was…

Study snapshot

PublicationBratislavske lekarske listy · 2015
Study typeanimal
Reported directionMixed
Evidence ratingLow

Abstract

OBJECTIVES: We aimed to clarify if melatonin treatment (2 mg/kg i.p.) may favorably impact the liver tissue in rats exposed to microwave radiation. The experiment was performed on 84 six-weeks-old Wistar male rats exposed for 4h a day, for 20, 40 and 60 days, respectively, to microwaves (900 MHz, 100-300 microT, 54-160 V/m). Rats were divided in to four groups: I (control) - rats treated with saline, II (Mel) - rats treated with melatonin, III (MWs) - microwave exposed rats, IV (MWs + Mel) - MWs exposed rats treated with melatonin. We evaluated oxidative stress parameters (malondialdehyde and carbonyl group content), catalase, xanthine oxidase, deoxyribonuclease I and II activity. BACKGROUND: Oxidative stress is the key mechanism of the microwave induced tissue injury. Melatonin, a lipophilic indoleamine primarily synthesized and released from the pineal gland is a powerful antioxidant. RESULTS: Exposure to microwaves caused an increase in malondialdehyde after 40 (p < 0.01), protein carbonyl content after 20 (p < 0.05), catalase (p < 0.05) and xantine oxidase activity (p < 0.05) after 40 days. Increase in deoxyribonuclease I activity was observed after 60 days (p < 0.05), while deoxyribonuclease II activity was unaffected. Melatonin treatment led to malondialdehyde decrease after 40 days (p< 0.05), but surprisingly had no effect on other analyzed parameters. CONCLUSION: Melatonin exerts certain antioxidant effects in the liver of rats exposed to microwaves, by diminishing the intensity of lipid peroxidation(Fig. 6, Ref. 32).

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AI evidence extraction

Structured fields below were generated from the paper’s abstract and metadata to make the evidence easier to inspect. Automated extraction may be incomplete or incorrect; verify important details against the original paper.

Study typeanimal
Effect directionmixed
PopulationSix-weeks-old Wistar male rats
Sample size84
ExposureRF · 900 MHz · 4 h/day for 20, 40, or 60 days
Evidence strengthLowConfidence: 78% · Peer reviewed: yes

Main findings

Microwave exposure (900 MHz) increased malondialdehyde (after 40 days), protein carbonyl content (after 20 days), catalase and xanthine oxidase activity (after 40 days), and deoxyribonuclease I activity (after 60 days), while deoxyribonuclease II activity was unaffected. Melatonin (2 mg/kg i.p.) decreased malondialdehyde after 40 days but had no effect on the other analyzed parameters.

Outcomes measured

  • Liver oxidative stress parameters (malondialdehyde, protein carbonyl content)
  • Catalase activity
  • Xanthine oxidase activity
  • Deoxyribonuclease I activity
  • Deoxyribonuclease II activity

Limitations

  • No SAR reported
  • Exposure source not specified
  • Only male rats studied
  • Outcomes limited to biochemical/enzymatic markers in liver; no clinical or histopathological outcomes described in abstract
  • Melatonin showed effect on only one parameter (malondialdehyde)
View raw extracted JSON
{
  "study_type": "animal",
  "exposure": {
    "band": "RF",
    "source": null,
    "frequency_mhz": 900,
    "sar_wkg": null,
    "duration": "4 h/day for 20, 40, or 60 days"
  },
  "population": "Six-weeks-old Wistar male rats",
  "sample_size": 84,
  "outcomes": [
    "Liver oxidative stress parameters (malondialdehyde, protein carbonyl content)",
    "Catalase activity",
    "Xanthine oxidase activity",
    "Deoxyribonuclease I activity",
    "Deoxyribonuclease II activity"
  ],
  "main_findings": "Microwave exposure (900 MHz) increased malondialdehyde (after 40 days), protein carbonyl content (after 20 days), catalase and xanthine oxidase activity (after 40 days), and deoxyribonuclease I activity (after 60 days), while deoxyribonuclease II activity was unaffected. Melatonin (2 mg/kg i.p.) decreased malondialdehyde after 40 days but had no effect on the other analyzed parameters.",
  "effect_direction": "mixed",
  "limitations": [
    "No SAR reported",
    "Exposure source not specified",
    "Only male rats studied",
    "Outcomes limited to biochemical/enzymatic markers in liver; no clinical or histopathological outcomes described in abstract",
    "Melatonin showed effect on only one parameter (malondialdehyde)"
  ],
  "evidence_strength": "low",
  "confidence": 0.78,
  "peer_reviewed_likely": "yes",
  "keywords": [
    "melatonin",
    "liver",
    "rat",
    "Wistar",
    "microwave radiation",
    "900 MHz",
    "oxidative stress",
    "malondialdehyde",
    "protein carbonyl",
    "catalase",
    "xanthine oxidase",
    "DNase I",
    "DNase II"
  ],
  "suggested_hubs": []
}
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AI-extracted fields are generated from the abstract and metadata and may be incomplete or incorrect. This material is for informational purposes and is not medical advice.

Identifiers and source

DOI: 10.4149/bll_2015_018
PubMed: 25665474

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