Apoptosis induced by microwave radiation in pancreatic cancer JF305 cells.

Canadian journal of physiology and pharmacology · 2014 · Benefit · Low evidence

← Research stream

BenefitLow evidencePaper 6562

Microwave exposure (2.5–20.0 mW/cm(2) for 20 min) inhibited JF305 pancreatic cancer cell growth in a dose-dependent manner and increased apoptosis (annexin V-FITC: 4.0% at 0, 10.0% at 5.0, 12.0% at 10.0, and 30.0% at 20.0 mW/cm(2)). Microwave treatment increa…

Study snapshot

PublicationCanadian journal of physiology and pharmacology · 2014

Study typein_vitro

Reported directionBenefit

Evidence ratingLow

Abstract

New therapeutic approaches are needed to improve the survival rate from pancreatic cancer, one of the most lethal human malignancies. In this study, JF305 cells were treated with microwaves at doses of 2.5, 5.0, 10.0, 15.0, and 20.0 mW/cm(2) for 20 min. The inhibition of JF305 cell proliferation was tested using the MTT assay. Apoptotic cells were detected with Hoechst 33258 staining and a Nucleo-Counter NC-3000. The expression of apoptosis-related proteins was examined with Western blot. The results showed that microwaves inhibited the growth of JF305 cells in a dose-dependent manner, and caused morphological changes in apoptotic body formation. The percentages of apoptosis detected using annexin V-fluorescein isothiocyanate (FITC) were 4.0%, 10.0%, 12.0%, and 30.0% with the dosage of microwave (0, 5.0, 10.0, and 20.0 mW/cm(2)), respectively. Treatment with microwaves increased the activity of caspase-9 and caspase-3, down-regulated the expression of Bcl-2, and up-regulated the expression of Bax and CytoC. In addition, the expression level of p65 was increased whereas the level of IκBα down-regulated. Those results suggest that microwaves inhibit cell growth and induce apoptosis in JF305 cells through an NF-κB-regulated mitochondria-mediated pathway.

Machine Enhanced Logic

AI evidence extraction

Structured fields below were generated from the paper’s abstract and metadata to make the evidence easier to inspect. Automated extraction may be incomplete or incorrect; verify important details against the original paper.

Study typein_vitro

Effect directionbenefit

PopulationNot supplied

Sample sizeNot supplied

Exposuremicrowave · 20 min

Evidence strengthLowConfidence: 78% · Peer reviewed: yes

Main findings

Outcomes measured

JF305 cell proliferation (MTT assay)
Apoptosis (Hoechst 33258 staining; Nucleo-Counter NC-3000; annexin V-FITC)
Caspase-9 activity
Caspase-3 activity
Bcl-2 expression
Bax expression
CytoC expression
NF-κB pathway markers (p65, IκBα)

Limitations

Frequency of microwave radiation not reported in abstract
SAR not reported in abstract
Sample size/replicates not reported in abstract
In vitro cancer cell line study; generalizability to humans not addressed in abstract

View raw extracted JSON

{
  "study_type": "in_vitro",
  "exposure": {
    "band": "microwave",
    "source": null,
    "frequency_mhz": null,
    "sar_wkg": null,
    "duration": "20 min"
  },
  "population": null,
  "sample_size": null,
  "outcomes": [
    "JF305 cell proliferation (MTT assay)",
    "Apoptosis (Hoechst 33258 staining; Nucleo-Counter NC-3000; annexin V-FITC)",
    "Caspase-9 activity",
    "Caspase-3 activity",
    "Bcl-2 expression",
    "Bax expression",
    "CytoC expression",
    "NF-κB pathway markers (p65, IκBα)"
  ],
  "main_findings": "Microwave exposure (2.5–20.0 mW/cm(2) for 20 min) inhibited JF305 pancreatic cancer cell growth in a dose-dependent manner and increased apoptosis (annexin V-FITC: 4.0% at 0, 10.0% at 5.0, 12.0% at 10.0, and 30.0% at 20.0 mW/cm(2)). Microwave treatment increased caspase-9 and caspase-3 activity, decreased Bcl-2, increased Bax and CytoC, increased p65, and decreased IκBα.",
  "effect_direction": "benefit",
  "limitations": [
    "Frequency of microwave radiation not reported in abstract",
    "SAR not reported in abstract",
    "Sample size/replicates not reported in abstract",
    "In vitro cancer cell line study; generalizability to humans not addressed in abstract"
  ],
  "evidence_strength": "low",
  "confidence": 0.78,
  "peer_reviewed_likely": "yes",
  "keywords": [
    "microwave radiation",
    "pancreatic cancer",
    "JF305 cells",
    "apoptosis",
    "cell proliferation",
    "caspase-3",
    "caspase-9",
    "Bcl-2",
    "Bax",
    "Cytochrome c",
    "NF-κB",
    "p65",
    "IκBα"
  ],
  "suggested_hubs": []
}

AI can be wrong. Always verify against the paper.

AI-extracted fields are generated from the abstract and metadata and may be incomplete or incorrect. This material is for informational purposes and is not medical advice.

Identifiers and source

DOI: 10.1139/cjpp-2013-0220
PubMed: 24708215

Read the original paper →

Related papers

← Back to the Research Library