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Advanced laboratory techniques for sample processing and immunolabeling using microwave radiation.

Journal of neuroscience methods · 2009 · Unclear · Insufficient evidence

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UnclearInsufficient evidencePaper 6489

Microwave-assisted immunohistochemistry enabled sequential labeling with several primary antibodies in a very short period of time with excellent labeling characteristics. The study also reports results on microwave-assisted formaldehyde fixation and its effe…

Study snapshot

PublicationJournal of neuroscience methods · 2009
Study typeother
Reported directionUnclear
Evidence ratingInsufficient

Abstract

A better understanding of improved microwave technology has increased the benefits and versatility of the technique as it applies to all aspects of immunohistochemistry. The role of continuous magnetron power output (wattage) combined with precise control of sample heating demonstrated their significance to complex labeling protocols. Here, we present results for microwave-assisted formaldehyde fixation and its effect on GFP expression in transfected HeLa cells. Rat brain sections and cultured hippocampal cells were labeled with 11 different primary antibodies using a unified microwave protocol. Microwave-assisted immunohistochemistry made it possible to sequentially label tissues and cells with several primary antibodies in a very short period of time with excellent labeling characteristics.

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AI evidence extraction

Structured fields below were generated from the paper’s abstract and metadata to make the evidence easier to inspect. Automated extraction may be incomplete or incorrect; verify important details against the original paper.

Study typeother
Effect directionunclear
PopulationTransfected HeLa cells; rat brain sections; cultured hippocampal cells
Sample sizeNot supplied
Exposuremicrowave
Evidence strengthInsufficientConfidence: 66% · Peer reviewed: yes

Main findings

Microwave-assisted immunohistochemistry enabled sequential labeling with several primary antibodies in a very short period of time with excellent labeling characteristics. The study also reports results on microwave-assisted formaldehyde fixation and its effect on GFP expression in transfected HeLa cells.

Outcomes measured

  • Effect of microwave-assisted formaldehyde fixation on GFP expression in transfected HeLa cells
  • Immunolabeling performance/labeling characteristics using 11 different primary antibodies in rat brain sections and cultured hippocampal cells
  • Ability to sequentially label tissues/cells with several primary antibodies in a short period of time
View raw extracted JSON
{
  "study_type": "other",
  "exposure": {
    "band": "microwave",
    "source": "laboratory microwave radiation (microwave-assisted immunohistochemistry)",
    "frequency_mhz": null,
    "sar_wkg": null,
    "duration": null
  },
  "population": "Transfected HeLa cells; rat brain sections; cultured hippocampal cells",
  "sample_size": null,
  "outcomes": [
    "Effect of microwave-assisted formaldehyde fixation on GFP expression in transfected HeLa cells",
    "Immunolabeling performance/labeling characteristics using 11 different primary antibodies in rat brain sections and cultured hippocampal cells",
    "Ability to sequentially label tissues/cells with several primary antibodies in a short period of time"
  ],
  "main_findings": "Microwave-assisted immunohistochemistry enabled sequential labeling with several primary antibodies in a very short period of time with excellent labeling characteristics. The study also reports results on microwave-assisted formaldehyde fixation and its effect on GFP expression in transfected HeLa cells.",
  "effect_direction": "unclear",
  "limitations": [],
  "evidence_strength": "insufficient",
  "confidence": 0.66,
  "peer_reviewed_likely": "yes",
  "keywords": [
    "microwave radiation",
    "immunohistochemistry",
    "microwave-assisted fixation",
    "formaldehyde fixation",
    "GFP expression",
    "HeLa cells",
    "rat brain sections",
    "hippocampal cells",
    "primary antibodies",
    "sample heating",
    "magnetron power output"
  ],
  "suggested_hubs": []
}
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AI-extracted fields are generated from the abstract and metadata and may be incomplete or incorrect. This material is for informational purposes and is not medical advice.

Identifiers and source

DOI: 10.1016/j.jneumeth.2009.06.002
PubMed: 19520116

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