Effect of radiofrequency radiation on mRNA expression in cultured rodent cells.

Physiological chemistry and physics and medical NMR · 1988 · No effect · Low evidence

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No effectLow evidencePaper 6243

Across four rodent cell lines and the DNA probes used (oncogenes, heat shock protein, long terminal repeat sequences), no significant differences in mRNA expression were observed between microwave-exposed and sham-exposed samples.

Study snapshot

PublicationPhysiological chemistry and physics and medical NMR · 1988

Study typein_vitro

Reported directionNo effect

Evidence ratingLow

Abstract

Four rodent cell lines were exposed to 2450 MHz microwave radiation at a Specific Absorption Rate (SAR) of 103.5 +/- 4.2 W/kg for varying lengths of time at 37 degrees, 40 degrees, 42 degrees and 45 degrees C. mRNA was extracted from microwave-exposed and sham-exposed cells and dot blotted or Northern blotted to nitrocellulose. Radioisotope labelled DNA probes of oncogenes, heat shock protein or long terminal repeat sequences were hybridized to the mRNA, and the resulting autoradiographs analyzed for differences in levels of mRNA expression between exposed and nonexposed samples. With the cell lines and probes used in this study no significant differences in mRNA expression were observed after microwave exposure.

Machine Enhanced Logic

AI evidence extraction

Structured fields below were generated from the paper’s abstract and metadata to make the evidence easier to inspect. Automated extraction may be incomplete or incorrect; verify important details against the original paper.

Study typein_vitro

Effect directionno_effect

PopulationFour rodent cell lines (cultured)

Sample sizeNot supplied

Exposuremicrowave · 2450 MHz · 103.5 W/kg · varying lengths of time

Evidence strengthLowConfidence: 78% · Peer reviewed: yes

Main findings

Outcomes measured

mRNA expression (oncogenes)
mRNA expression (heat shock protein)
mRNA expression (long terminal repeat sequences)

Limitations

In vitro study (cultured rodent cell lines)
Exposure durations not specified in the abstract
Only selected mRNA targets/probes were assessed
High SAR exposure level; generalizability to other exposure conditions not addressed in abstract

View raw extracted JSON

{
  "study_type": "in_vitro",
  "exposure": {
    "band": "microwave",
    "source": null,
    "frequency_mhz": 2450,
    "sar_wkg": 103.5,
    "duration": "varying lengths of time"
  },
  "population": "Four rodent cell lines (cultured)",
  "sample_size": null,
  "outcomes": [
    "mRNA expression (oncogenes)",
    "mRNA expression (heat shock protein)",
    "mRNA expression (long terminal repeat sequences)"
  ],
  "main_findings": "Across four rodent cell lines and the DNA probes used (oncogenes, heat shock protein, long terminal repeat sequences), no significant differences in mRNA expression were observed between microwave-exposed and sham-exposed samples.",
  "effect_direction": "no_effect",
  "limitations": [
    "In vitro study (cultured rodent cell lines)",
    "Exposure durations not specified in the abstract",
    "Only selected mRNA targets/probes were assessed",
    "High SAR exposure level; generalizability to other exposure conditions not addressed in abstract"
  ],
  "evidence_strength": "low",
  "confidence": 0.78,
  "peer_reviewed_likely": "yes",
  "keywords": [
    "microwave radiation",
    "radiofrequency",
    "2450 MHz",
    "SAR",
    "rodent cell lines",
    "mRNA expression",
    "oncogenes",
    "heat shock protein",
    "Northern blot",
    "dot blot",
    "sham-exposed"
  ],
  "suggested_hubs": []
}

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AI-extracted fields are generated from the abstract and metadata and may be incomplete or incorrect. This material is for informational purposes and is not medical advice.

Identifiers and source

DOI: Not supplied
PubMed: 3222348

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