Evaluation of Cell Migration and Cytokines Expression Changes under the Radiofrequency Electromagnetic Field on Wound Healing In Vitro Model.

International journal of molecular sciences · 2022 · Benefit · Low evidence

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BenefitLow evidencePaper 5752

In an in vitro wound healing model, RF-EMF treatment promoted keratinocyte migration and regulated expression of genes involved in healing, including MMPs, tissue inhibitors of metalloproteinases, and pro/anti-inflammatory cytokines, consistent with improved …

Study snapshot

PublicationInternational journal of molecular sciences · 2022

Study typein_vitro

Reported directionBenefit

Evidence ratingLow

Abstract

Wound healing (WH) proceeds through four distinct phases: hemostasis, inflammation, proliferation, and remodeling. Impaired WH may be the consequence of the alteration of one of these phases and represents a significant health and economic burden to millions of individuals. Thus, new therapeutic strategies are the topics of intense research worldwide. Although radiofrequency electromagnetic field (RF-EMF) has many medical applications in rehabilitation, pain associated with musculoskeletal disorders, and degenerative joint disorders, its impact on WH is not fully understood. The process of WH begins just after injury and continues during the inflammatory and proliferative phases. A thorough understanding of the mechanisms by which RF-EMF can improve WH is required before it can be used as a non-invasive, inexpensive, and easily self-applicable therapeutic strategy. Thus, the aim of this study is to explore the therapeutic potential of different exposure setups of RF-EMF to drive faster healing, evaluating the keratinocytes migration, cytokines, and matrix metalloproteinases (MMPs) expression. The results showed that RF-EMF treatment promotes keratinocytes' migration and regulates the expression of genes involved in healing, such as MMPs, tissue inhibitors of metalloproteinases, and pro/anti-inflammatory cytokines, to improve WH.

Machine Enhanced Logic

AI evidence extraction

Structured fields below were generated from the paper’s abstract and metadata to make the evidence easier to inspect. Automated extraction may be incomplete or incorrect; verify important details against the original paper.

Study typein_vitro

Effect directionbenefit

PopulationNot supplied

Sample sizeNot supplied

ExposureRF

Evidence strengthLowConfidence: 74% · Peer reviewed: yes

Main findings

Outcomes measured

keratinocyte migration
cytokine gene expression (pro-/anti-inflammatory)
matrix metalloproteinases (MMPs) expression
tissue inhibitors of metalloproteinases expression
in vitro wound healing model outcomes

Limitations

RF-EMF exposure parameters (e.g., frequency, SAR, duration) not specified in the abstract
In vitro model; findings may not translate to in vivo or clinical wound healing
Sample size and experimental details not provided in the abstract

View raw extracted JSON

{
  "study_type": "in_vitro",
  "exposure": {
    "band": "RF",
    "source": null,
    "frequency_mhz": null,
    "sar_wkg": null,
    "duration": null
  },
  "population": null,
  "sample_size": null,
  "outcomes": [
    "keratinocyte migration",
    "cytokine gene expression (pro-/anti-inflammatory)",
    "matrix metalloproteinases (MMPs) expression",
    "tissue inhibitors of metalloproteinases expression",
    "in vitro wound healing model outcomes"
  ],
  "main_findings": "In an in vitro wound healing model, RF-EMF treatment promoted keratinocyte migration and regulated expression of genes involved in healing, including MMPs, tissue inhibitors of metalloproteinases, and pro/anti-inflammatory cytokines, consistent with improved wound healing.",
  "effect_direction": "benefit",
  "limitations": [
    "RF-EMF exposure parameters (e.g., frequency, SAR, duration) not specified in the abstract",
    "In vitro model; findings may not translate to in vivo or clinical wound healing",
    "Sample size and experimental details not provided in the abstract"
  ],
  "evidence_strength": "low",
  "confidence": 0.74,
  "peer_reviewed_likely": "yes",
  "keywords": [
    "radiofrequency electromagnetic field",
    "RF-EMF",
    "wound healing",
    "in vitro",
    "keratinocytes",
    "cell migration",
    "cytokines",
    "matrix metalloproteinases",
    "MMPs",
    "gene expression"
  ],
  "suggested_hubs": []
}

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AI-extracted fields are generated from the abstract and metadata and may be incomplete or incorrect. This material is for informational purposes and is not medical advice.

Identifiers and source

DOI: 10.3390/ijms23042205
PubMed: 35216321

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