Electromagnetic Fields Generated by the IteraCoil Device Differentiate Mesenchymal Stem Progenitor Cells Into the Osteogenic Lineage.

Bioelectromagnetics · 2022 · Benefit · Very low evidence

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BenefitVery low evidencePaper 5746

Study snapshot

PublicationBioelectromagnetics · 2022

Study typein_vitro

Reported directionBenefit

Evidence ratingVery low

Abstract

Rapid advances in mesenchymal stem progenitor cells (MSPCs) have rendered impetus into the area of cell therapy and regenerative medicine. The main promise of future stem cell therapies is their reliance on autologous stem cells derived from adipose tissue, which also includes treatments of bone fractures and degeneration. The effectiveness of different electric devices utilized to reprogram MSPCs toward osteogenic differentiation has provided varying degrees of effectiveness for clinical use. Adipose tissue-derived MSPCs were flow-cytometrically characterized and further differentiated into osteoblasts by culturing either in growth medium with pro-osteogenic supplements or without supplements with alternating electromagnetic field (EMF) generated by IteraCoil. IteraCoil is a multi-solenoid coil with a specific complex geometry that creates a 3D-EMF with desired parameters without directly applying electrodes to the cells and tissues. The flow-cytometric analysis of highly enriched (≥95%) adipose-derived MSPCs (CD34 , CD73 , CD90 , and CD105 ) was utilized for the study. Osteoblasts and chondrocyte differentiations were then assessed by specific staining and quantified using ImageJ (National Institutes of Health). The osteoblastic differentiation of MSPCs cultured in regular medium and exposed to EMF at 0.05 and 1 kHz frequencies was compared with MSPCs cultured in a pro-osteogenic supplemented medium. In this study, we demonstrated that EMF from IteraCoil might have affected the signaling pathways that induce the osteogenic differentiation of human adipose-derived MSPCs in the absence of exogenous osteogenic factors. Therefore, EMF-generated osteogenic differentiation of reprogrammed adipose-derived autologous MSPCs may treat the loss of osteoblasts and osteoporosis and open new avenues for the development of regenerative cellular therapy. © 2022 Bioelectromagnetics Society.

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AI evidence extraction

Structured fields below were generated from the paper’s abstract and metadata to make the evidence easier to inspect. Automated extraction may be incomplete or incorrect; verify important details against the original paper.

Study typein_vitro

Effect directionbenefit

PopulationHuman adipose tissue-derived mesenchymal stem progenitor cells (MSPCs)

Sample sizeNot supplied

ExposureNot supplied

Evidence strengthVery lowConfidence: 74% · Peer reviewed: yes

Main findings

Adipose-derived human MSPCs exposed to alternating EMF generated by the IteraCoil (0.05 and 1 kHz) showed osteoblastic differentiation in regular medium without exogenous pro-osteogenic supplements, compared against cells differentiated in pro-osteogenic supplemented medium. The authors report the EMF might have affected signaling pathways inducing osteogenic differentiation.

Outcomes measured

Osteogenic (osteoblastic) differentiation
Chondrocyte differentiation
Flow-cytometric characterization of MSPCs (CD34, CD73, CD90, CD105)
Staining-based differentiation assessment quantified with ImageJ

Limitations

In vitro study; findings may not translate to clinical outcomes
Sample size not reported in the abstract
Exposure duration and detailed EMF parameters beyond frequency are not reported in the abstract

View raw extracted JSON

{
  "study_type": "in_vitro",
  "exposure": {
    "band": null,
    "source": "IteraCoil device (multi-solenoid coil generating alternating EMF)",
    "frequency_mhz": null,
    "sar_wkg": null,
    "duration": null
  },
  "population": "Human adipose tissue-derived mesenchymal stem progenitor cells (MSPCs)",
  "sample_size": null,
  "outcomes": [
    "Osteogenic (osteoblastic) differentiation",
    "Chondrocyte differentiation",
    "Flow-cytometric characterization of MSPCs (CD34, CD73, CD90, CD105)",
    "Staining-based differentiation assessment quantified with ImageJ"
  ],
  "main_findings": "Adipose-derived human MSPCs exposed to alternating EMF generated by the IteraCoil (0.05 and 1 kHz) showed osteoblastic differentiation in regular medium without exogenous pro-osteogenic supplements, compared against cells differentiated in pro-osteogenic supplemented medium. The authors report the EMF might have affected signaling pathways inducing osteogenic differentiation.",
  "effect_direction": "benefit",
  "limitations": [
    "In vitro study; findings may not translate to clinical outcomes",
    "Sample size not reported in the abstract",
    "Exposure duration and detailed EMF parameters beyond frequency are not reported in the abstract"
  ],
  "evidence_strength": "very_low",
  "confidence": 0.74,
  "peer_reviewed_likely": "yes",
  "keywords": [
    "IteraCoil",
    "alternating electromagnetic field",
    "mesenchymal stem progenitor cells",
    "adipose-derived",
    "osteogenic differentiation",
    "osteoblasts",
    "regenerative medicine",
    "in vitro",
    "kHz"
  ],
  "suggested_hubs": []
}

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AI-extracted fields are generated from the abstract and metadata and may be incomplete or incorrect. This material is for informational purposes and is not medical advice.

Identifiers and source

DOI: 10.1002/bem.22401
PubMed: 35391494

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