Improved osteogenic differentiation by extremely low electromagnetic field exposure: possible application for bone engineering.

Histochemistry and cell biology · 2022 · Benefit · Low evidence

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BenefitLow evidencePaper 5736

Compared with sham exposure, intermittent ELF-EMF exposure increased cell proliferation rate and de novo calcium deposition after 10 days (both p<0.001). COL1A1 and RUNX-2 gene expression and COL1A1, RUNX-2, and OPN protein expression were upregulated in ELF-…

Study snapshot

PublicationHistochemistry and cell biology · 2022

Study typein_vitro

Reported directionBenefit

Evidence ratingLow

Abstract

Human periodontal ligament mesenchymal stem cells (hPDLSCs) are a promising cell type model for regenerative medicine applications due to their anti-inflammatory, immunomodulatory and non-tumorigenic potentials. Extremely low-frequency electromagnetic fields (ELF-EMF) are reported to affect biological properties such as cell proliferation and differentiation and modulate gene expression profile. In this study, we investigated the effects of an intermittent ELF-EMF exposure (6 h/day) for the standard differentiation period (28 days) and for 10 days in hPDLSCs in the presence or not of osteogenic differentiation medium (OM). We evaluated cell proliferation, de novo calcium deposition and osteogenic differentiation marker expression in sham and ELF-EMF-exposed cells. After ELF-EMF exposure, compared with sham-exposed, an increase in cell proliferation rate (p < 0.001) and de novo calcium deposition (p < 0.001) was observed after 10 days of exposure. Real-time PCR and Western blot results showed that COL1A1 and RUNX-2 gene expression and COL1A1, RUNX-2 and OPN protein expression were upregulated respectively in the cells exposed to ELF-EMF exposure along with or without OM for 10 days. Altogether, these results suggested that the promotion of osteogenic differentiation is more efficient in ELF-EMF-exposed hPDLSCs. Moreover, our analyses indicated that there is an early induction of hPDLSC differentiation after ELF-EMF application.

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AI evidence extraction

Structured fields below were generated from the paper’s abstract and metadata to make the evidence easier to inspect. Automated extraction may be incomplete or incorrect; verify important details against the original paper.

Study typein_vitro

Effect directionbenefit

PopulationHuman periodontal ligament mesenchymal stem cells (hPDLSCs)

Sample sizeNot supplied

ExposureELF · intermittent exposure 6 h/day for 10 days and for 28 days

Evidence strengthLowConfidence: 74% · Peer reviewed: yes

Main findings

Outcomes measured

Cell proliferation
De novo calcium deposition
Osteogenic differentiation marker expression (COL1A1, RUNX-2, OPN)

Limitations

In vitro study (cell culture), limiting direct inference to human health outcomes
Key exposure parameters (e.g., field strength, frequency) not reported in the provided abstract
Sample size and replication details not reported in the provided abstract

View raw extracted JSON

{
  "study_type": "in_vitro",
  "exposure": {
    "band": "ELF",
    "source": null,
    "frequency_mhz": null,
    "sar_wkg": null,
    "duration": "intermittent exposure 6 h/day for 10 days and for 28 days"
  },
  "population": "Human periodontal ligament mesenchymal stem cells (hPDLSCs)",
  "sample_size": null,
  "outcomes": [
    "Cell proliferation",
    "De novo calcium deposition",
    "Osteogenic differentiation marker expression (COL1A1, RUNX-2, OPN)"
  ],
  "main_findings": "Compared with sham exposure, intermittent ELF-EMF exposure increased cell proliferation rate and de novo calcium deposition after 10 days (both p<0.001). COL1A1 and RUNX-2 gene expression and COL1A1, RUNX-2, and OPN protein expression were upregulated in ELF-EMF-exposed cells with or without osteogenic medium, suggesting earlier/more efficient osteogenic differentiation.",
  "effect_direction": "benefit",
  "limitations": [
    "In vitro study (cell culture), limiting direct inference to human health outcomes",
    "Key exposure parameters (e.g., field strength, frequency) not reported in the provided abstract",
    "Sample size and replication details not reported in the provided abstract"
  ],
  "evidence_strength": "low",
  "confidence": 0.74,
  "peer_reviewed_likely": "yes",
  "keywords": [
    "extremely low-frequency electromagnetic fields",
    "ELF-EMF",
    "mesenchymal stem cells",
    "periodontal ligament",
    "osteogenic differentiation",
    "bone engineering",
    "calcium deposition",
    "COL1A1",
    "RUNX-2",
    "OPN"
  ],
  "suggested_hubs": []
}

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AI-extracted fields are generated from the abstract and metadata and may be incomplete or incorrect. This material is for informational purposes and is not medical advice.

Identifiers and source

DOI: 10.1007/s00418-022-02126-9
PubMed: 35751679

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