First Identification of the Effects of Low Frequency Electromagnetic Field on the Micromolecular Changes in Adipose Tissue-Derived Mesenchymal Stem Cells by Fourier Transform Infrared Spectroscopy.

Journal of medical physics · 2021 · Mixed · Low evidence

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MixedLow evidencePaper 5660

AT-MSCs exposed to a 50 Hz, 1.5 mT sinusoidal EMF showed the greatest FTIR spectral differences versus control at 24 and 48 hours, particularly in nucleic acids and proteins in the fingerprint region. After 72 hours of exposure, no significant FTIR spectral d…

Study snapshot

PublicationJournal of medical physics · 2021

Study typein_vitro

Reported directionMixed

Evidence ratingLow

Abstract

PURPOSE: In this study, we hypothesize that exposure of adipose tissue-mesenchymal stem cells (AT-MSCs) to electromagnetic field (EMF) may impact adipose stem cells' micromolecular structure (analyzed using Fourier transform infrared spectroscopy [FTIR]). MATERIALS AND METHODS: The AT-MSCs were exposed to continuous vertically applied sinusoidal EMF with a frequency of 50 Hz and a flux density of 1.5 mT for 24, 48, and 72 h. After an appropriate time (24, 48, 72 h) cells were washed with PBS, scrubbed, and immediately taken into FTIR analyses. RESULTS: EMFs affect AT-MSCs. The greatest differences were in the range of nucleic acids and proteins in the fingerprint region which occurred after 24 and 48 h of EMF exposure. However, in the case of 72 h of EMF exposure, no significant differences were noticed in the FTIR spectra towards the control. CONCLUSIONS: FTIR spectra show differences between samples under the influence of EMF before they will be manifested at the morphological level. The largest differences in the range of nucleic acids and proteins in the fingerprint region occurred at 24 and 48 h of EMF exposure. That means it was during the first 48 h after EMF exposure a great number of dynamic changes occurred. However, in the case of AT-MSCs in 72 h EMF and 72 h control, no significant differences were noted in the FTIR spectra, which means that the chemical composition in these two cases is similar. EMF is not neutral for stem cells, especially in the in the first hours of interaction (24 h, 48 h).

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AI evidence extraction

Structured fields below were generated from the paper’s abstract and metadata to make the evidence easier to inspect. Automated extraction may be incomplete or incorrect; verify important details against the original paper.

Study typein_vitro

Effect directionmixed

PopulationAdipose tissue-derived mesenchymal stem cells (AT-MSCs)

Sample sizeNot supplied

ExposureELF · 0.05 MHz · 24, 48, and 72 h

Evidence strengthLowConfidence: 78% · Peer reviewed: yes

Main findings

Outcomes measured

FTIR spectral changes (micromolecular structure)
Nucleic acids and proteins spectral features in the fingerprint region

Limitations

Sample size not reported in abstract
Only FTIR-based micromolecular/spectral endpoints reported; no direct functional or morphological outcomes described
Exposure conditions limited to one frequency (50 Hz) and one flux density (1.5 mT)
In vitro study; generalizability to in vivo/human health outcomes not addressed

View raw extracted JSON

{
  "study_type": "in_vitro",
  "exposure": {
    "band": "ELF",
    "source": null,
    "frequency_mhz": 0.05,
    "sar_wkg": null,
    "duration": "24, 48, and 72 h"
  },
  "population": "Adipose tissue-derived mesenchymal stem cells (AT-MSCs)",
  "sample_size": null,
  "outcomes": [
    "FTIR spectral changes (micromolecular structure)",
    "Nucleic acids and proteins spectral features in the fingerprint region"
  ],
  "main_findings": "AT-MSCs exposed to a 50 Hz, 1.5 mT sinusoidal EMF showed the greatest FTIR spectral differences versus control at 24 and 48 hours, particularly in nucleic acids and proteins in the fingerprint region. After 72 hours of exposure, no significant FTIR spectral differences versus control were observed.",
  "effect_direction": "mixed",
  "limitations": [
    "Sample size not reported in abstract",
    "Only FTIR-based micromolecular/spectral endpoints reported; no direct functional or morphological outcomes described",
    "Exposure conditions limited to one frequency (50 Hz) and one flux density (1.5 mT)",
    "In vitro study; generalizability to in vivo/human health outcomes not addressed"
  ],
  "evidence_strength": "low",
  "confidence": 0.78,
  "peer_reviewed_likely": "yes",
  "keywords": [
    "extremely low frequency",
    "ELF-EMF",
    "50 Hz",
    "1.5 mT",
    "mesenchymal stem cells",
    "adipose-derived stem cells",
    "FTIR",
    "nucleic acids",
    "proteins",
    "fingerprint region"
  ],
  "suggested_hubs": []
}

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AI-extracted fields are generated from the abstract and metadata and may be incomplete or incorrect. This material is for informational purposes and is not medical advice.

Identifiers and source

DOI: 10.4103/jmp.jmp_57_21
PubMed: 35261495

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