Characterizing the Binding of Angiotensin Converting Enzyme I Inhibitory Peptide to Human Hemoglobin: Influence of Electromagnetic Fields.

Protein and peptide letters · 2020 · Mixed · Very low evidence

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MixedVery low evidencePaper 5643

Spectroscopic measurements indicated that electromagnetic fields (EMF) changed the interaction between human hemoglobin and an ACE inhibitory peptide. The α-helix content of hemoglobin decreased upon binding to ACEIP and solution conductivity increased upon b…

Study snapshot

PublicationProtein and peptide letters · 2020

Study typein_vitro

Reported directionMixed

Evidence ratingVery low

Abstract

BACKGROUND: Drug-protein complexes is one of the crucial factors when analyzing the pharmacokinetics and pharmacodynamics of a drug because they can affect the excretion, distribution, metabolism and interaction with target tissues. OBJECTIVES: The aim of this study was to investigate the interaction of human hemoglobin (Hb) and angiotensin I converting enzyme inhibitory peptide (ACEIP) in the absence and presence of different- frequency electromagnetic fields (EMF). METHODS: Various spectroscopic methods like fluorescence spectroscopy, ultraviolet, circular dichroism and conductometry techniques were applied to investigate Hb-ACEIP interaction in the absence and presence of EMF. RESULT: The presented spectroscopic studies indicated that EMF changed the interaction between Hb and ACEIP. The a-helix content of Hb decreased upon binding to ACEIP and conductivity of the solution enhanced upon binding. Based on Stern-Volmer equations, it could be stated that the Hb-ACEIP affinity was higher in the presence of EMF. CONCLUSION: It can be concluded that for patients who use the drug to control blood pressure, a low-frequency electromagnetic field would have a positive effect on the uptake of the drug.

Machine Enhanced Logic

AI evidence extraction

Structured fields below were generated from the paper’s abstract and metadata to make the evidence easier to inspect. Automated extraction may be incomplete or incorrect; verify important details against the original paper.

Study typein_vitro

Effect directionmixed

PopulationNot supplied

Sample sizeNot supplied

ExposureNot supplied

Evidence strengthVery lowConfidence: 74% · Peer reviewed: yes

Main findings

Outcomes measured

Hemoglobin (Hb)–ACE inhibitory peptide (ACEIP) binding/interaction
Hb secondary structure (α-helix content)
Solution conductivity
Binding affinity (Stern–Volmer analysis)

Limitations

EMF frequencies are described as different-frequency/low-frequency but not specified.
In vitro spectroscopic study; clinical implications are not directly tested in patients.
No sample size or replication details provided in the abstract.
Exposure metrics (e.g., field strength, SAR, duration) are not reported in the abstract.

View raw extracted JSON

{
  "study_type": "in_vitro",
  "exposure": {
    "band": null,
    "source": null,
    "frequency_mhz": null,
    "sar_wkg": null,
    "duration": null
  },
  "population": null,
  "sample_size": null,
  "outcomes": [
    "Hemoglobin (Hb)–ACE inhibitory peptide (ACEIP) binding/interaction",
    "Hb secondary structure (α-helix content)",
    "Solution conductivity",
    "Binding affinity (Stern–Volmer analysis)"
  ],
  "main_findings": "Spectroscopic measurements indicated that electromagnetic fields (EMF) changed the interaction between human hemoglobin and an ACE inhibitory peptide. The α-helix content of hemoglobin decreased upon binding to ACEIP and solution conductivity increased upon binding; Stern–Volmer analysis suggested higher Hb–ACEIP affinity in the presence of EMF.",
  "effect_direction": "mixed",
  "limitations": [
    "EMF frequencies are described as different-frequency/low-frequency but not specified.",
    "In vitro spectroscopic study; clinical implications are not directly tested in patients.",
    "No sample size or replication details provided in the abstract.",
    "Exposure metrics (e.g., field strength, SAR, duration) are not reported in the abstract."
  ],
  "evidence_strength": "very_low",
  "confidence": 0.74,
  "peer_reviewed_likely": "yes",
  "keywords": [
    "electromagnetic fields",
    "low-frequency EMF",
    "hemoglobin",
    "ACE inhibitory peptide",
    "drug-protein interaction",
    "fluorescence spectroscopy",
    "circular dichroism",
    "conductometry",
    "Stern–Volmer"
  ],
  "suggested_hubs": []
}

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AI-extracted fields are generated from the abstract and metadata and may be incomplete or incorrect. This material is for informational purposes and is not medical advice.

Identifiers and source

DOI: 10.2174/1871530320666200425203636
PubMed: 32334494

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