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Effects of 5-HT and 5-HT Receptor Agonists on Electromagnetic Field-Induced Analgesia in Rats.

Bioelectromagnetics · 2019 · Benefit · Low evidence

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BenefitLow evidencePaper 5583

Rats exposed to alternating 50 Hz, 5 mT EMF showed analgesic activity that was increased by serotonin HCl and DOI hydrochloride compared with the EMF group, while WAY 100635 and SB 204741 reduced analgesic activity in MF-exposed rats (all P<0.05). The authors…

Study snapshot

PublicationBioelectromagnetics · 2019
Study typeanimal
Reported directionBenefit
Evidence ratingLow

Abstract

Much evidence demonstrates the antinociceptive effect of magnetic fields (MFs). However, the analgesic action mechanism of the electromagnetic field (EMF) is not exactly understood. The aim of the present study was to investigate the effects of 5-HT and 5-HT receptor agonists (serotonin HCl and 2,5-dimethoxy-4-iodoamphetamine [DOI] hydrochloride) on EMF-induced analgesia. In total, 66 adult male Wistar albino rats with an average body mass of 225 ± 13 g were used in this study. The animals were subjected to repeated exposures of alternating 50 Hz and 5 mT EMF for 2 h a day for 15 days. Prior to analgesia tests, serotonin HCl (5-HT agonist) 4 mg/kg, WAY 100635 (5-HT antagonist) 0.04 mg/kg, DOI hydrochloride (5-HT receptor agonist) 4 mg/kg, and SB 204741 (5-HT antagonist) 0.5 mg/kg doses were injected into rats. For statistical analysis of the data, analysis of variance was used and multiple comparisons were determined by Tukey's test. Administration of serotonin HCl MF (5 mT)-exposed rats produced a significant increase in percent maximal possible effect (% MPE) as compared with EMF group (P < 0.05). On the contrary, injection of WAY 100635 to MF-exposed rats produced a significant decrease in analgesic activity (P < 0.05). Similarly, the administration of DOI hydrochloride significantly increased % MPE values as compared with the EMF group while SB 204741 reduced it (P < 0.05). In conclusion, our results suggested that serotonin 5-HT and 5-HT receptors play an important role in EMF-induced analgesia; however, further research studies are necessary to understand the mechanism. Bioelectromagnetics. 2019;40:319-330. © 2019 Bioelectromagnetics Society.

Machine Enhanced Logic

AI evidence extraction

Structured fields below were generated from the paper’s abstract and metadata to make the evidence easier to inspect. Automated extraction may be incomplete or incorrect; verify important details against the original paper.

Study typeanimal
Effect directionbenefit
PopulationAdult male Wistar albino rats
Sample size66
ExposureELF · 2 h/day for 15 days; alternating 50 Hz, 5 mT
Evidence strengthLowConfidence: 74% · Peer reviewed: yes

Main findings

Rats exposed to alternating 50 Hz, 5 mT EMF showed analgesic activity that was increased by serotonin HCl and DOI hydrochloride compared with the EMF group, while WAY 100635 and SB 204741 reduced analgesic activity in MF-exposed rats (all P<0.05). The authors conclude that 5-HT and 5-HT receptors play an important role in EMF-induced analgesia.

Outcomes measured

  • Analgesia/antinociception (% maximal possible effect, %MPE)
  • Effect of serotonergic drugs (5-HT agonist/antagonists; 5-HT receptor agonist/antagonist) on EMF-induced analgesia

Limitations

  • Analgesia mechanism described as not exactly understood
  • Authors state further research is necessary to understand the mechanism
  • Exposure described as alternating 50 Hz and 5 mT; other exposure characterization details not provided in abstract
View raw extracted JSON
{
  "study_type": "animal",
  "exposure": {
    "band": "ELF",
    "source": null,
    "frequency_mhz": null,
    "sar_wkg": null,
    "duration": "2 h/day for 15 days; alternating 50 Hz, 5 mT"
  },
  "population": "Adult male Wistar albino rats",
  "sample_size": 66,
  "outcomes": [
    "Analgesia/antinociception (% maximal possible effect, %MPE)",
    "Effect of serotonergic drugs (5-HT agonist/antagonists; 5-HT receptor agonist/antagonist) on EMF-induced analgesia"
  ],
  "main_findings": "Rats exposed to alternating 50 Hz, 5 mT EMF showed analgesic activity that was increased by serotonin HCl and DOI hydrochloride compared with the EMF group, while WAY 100635 and SB 204741 reduced analgesic activity in MF-exposed rats (all P<0.05). The authors conclude that 5-HT and 5-HT receptors play an important role in EMF-induced analgesia.",
  "effect_direction": "benefit",
  "limitations": [
    "Analgesia mechanism described as not exactly understood",
    "Authors state further research is necessary to understand the mechanism",
    "Exposure described as alternating 50 Hz and 5 mT; other exposure characterization details not provided in abstract"
  ],
  "evidence_strength": "low",
  "confidence": 0.74,
  "peer_reviewed_likely": "yes",
  "keywords": [
    "electromagnetic field",
    "magnetic field",
    "ELF",
    "50 Hz",
    "5 mT",
    "analgesia",
    "antinociception",
    "serotonin",
    "5-HT",
    "DOI",
    "WAY 100635",
    "SB 204741",
    "rat"
  ],
  "suggested_hubs": [
    {
      "slug": "animal-studies",
      "weight": 0.9,
      "reason": "In vivo rat experiment assessing EMF-induced analgesia and serotonergic modulation."
    }
  ]
}
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AI-extracted fields are generated from the abstract and metadata and may be incomplete or incorrect. This material is for informational purposes and is not medical advice.

Identifiers and source

DOI: 10.1002/bem.22196
PubMed: 31152464

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