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Calcium spiking activity and baseline calcium levels in ROS 17/2.8 cells exposed to extremely low frequency electromagnetic fields (ELF EMF).

International journal of radiation biology · 2001 · No effect · Low evidence

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No effectLow evidencePaper 5323

Probit plots from cells exposed to various sinusoidal ELF magnetic fields were virtually superimposable on plots from pre- and post-exposure sham/no-field periods. The authors report no evidence of effects (positive or negative) on average cytosolic calcium l…

Study snapshot

PublicationInternational journal of radiation biology · 2001
Study typein_vitro
Reported directionNo effect
Evidence ratingLow

Abstract

PURPOSE: To determine whether extremely low frequency electromagnetic fields can alter average free cytosolic calcium ion concentrations [Ca2+]i and transient increases in [Ca2+]i in populations of ROS 17/2.8 cells. MATERIALS AND METHODS: Cells loaded with the calcium-selective luminescent photoprotein, aequorin, were placed in the bottom of a sample chamber, which was inserted into the gap of a previously described air gap reactor system where they were exposed either to sinusoidal magnetic fields at a variety of frequencies and flux densities or to sham conditions. Real-time recordings of photon counts due to aequorin luminescence were obtained and data were analysed with the use of probit plots. RESULTS: Probit plots of data obtained from cells exposed to the various magnetic fields were virtually superimposable over the data obtained for the same cultures during pre- and post-exposure sham or no-field periods. CONCLUSION: These experiments provided no evidence for any effects of ELF EMF, either positive or negative, on either average [Ca2+]i or on transient increases in [Ca2+]i.

Machine Enhanced Logic

AI evidence extraction

Structured fields below were generated from the paper’s abstract and metadata to make the evidence easier to inspect. Automated extraction may be incomplete or incorrect; verify important details against the original paper.

Study typein_vitro
Effect directionno_effect
PopulationROS 17/2.8 cells
Sample sizeNot supplied
ExposureELF
Evidence strengthLowConfidence: 78% · Peer reviewed: yes

Main findings

Probit plots from cells exposed to various sinusoidal ELF magnetic fields were virtually superimposable on plots from pre- and post-exposure sham/no-field periods. The authors report no evidence of effects (positive or negative) on average cytosolic calcium levels or transient calcium increases.

Outcomes measured

  • Average free cytosolic calcium ion concentration ([Ca2+]i)
  • Transient increases in [Ca2+]i (calcium spiking activity)
  • Aequorin luminescence photon counts (real-time recordings)

Limitations

  • Exposure frequencies and flux densities are described as varied but not specified in the abstract.
  • Sample size and replication details are not provided in the abstract.
  • Exposure duration is not stated in the abstract.
View raw extracted JSON
{
  "study_type": "in_vitro",
  "exposure": {
    "band": "ELF",
    "source": "other",
    "frequency_mhz": null,
    "sar_wkg": null,
    "duration": null
  },
  "population": "ROS 17/2.8 cells",
  "sample_size": null,
  "outcomes": [
    "Average free cytosolic calcium ion concentration ([Ca2+]i)",
    "Transient increases in [Ca2+]i (calcium spiking activity)",
    "Aequorin luminescence photon counts (real-time recordings)"
  ],
  "main_findings": "Probit plots from cells exposed to various sinusoidal ELF magnetic fields were virtually superimposable on plots from pre- and post-exposure sham/no-field periods. The authors report no evidence of effects (positive or negative) on average cytosolic calcium levels or transient calcium increases.",
  "effect_direction": "no_effect",
  "limitations": [
    "Exposure frequencies and flux densities are described as varied but not specified in the abstract.",
    "Sample size and replication details are not provided in the abstract.",
    "Exposure duration is not stated in the abstract."
  ],
  "evidence_strength": "low",
  "confidence": 0.78,
  "peer_reviewed_likely": "yes",
  "keywords": [
    "ELF EMF",
    "extremely low frequency",
    "magnetic fields",
    "in vitro",
    "ROS 17/2.8",
    "calcium",
    "cytosolic calcium",
    "calcium spiking",
    "aequorin",
    "sham exposure",
    "probit plots"
  ],
  "suggested_hubs": []
}
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AI-extracted fields are generated from the abstract and metadata and may be incomplete or incorrect. This material is for informational purposes and is not medical advice.

Identifiers and source

DOI: 10.1080/09553000010013779
PubMed: 11236931

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