In vitro studies of the influence of ELF electromagnetic fields on the activity of soluble and insoluble peroxidase.

Bioelectromagnetics · 2003 · Mixed · Low evidence

← Research stream

MixedLow evidencePaper 5264

An ELF magnetic field (50 Hz, 1 mT) affected the inactivation rate of soluble horseradish peroxidase, while no effects were observed for insoluble (immobilized) enzyme derivatives prepared in gelatin or nylon membranes.

Study snapshot

PublicationBioelectromagnetics · 2003

Study typein_vitro

Reported directionMixed

Evidence ratingLow

Abstract

The influence of an extremely low frequency (ELF) magnetic field (50 Hz and 1 mT, EMF) on the activity of a soluble and insoluble horseradish peroxidase (E.C. 1.11.17) has been studied as a function of time. Insoluble derivatives were obtained by enzyme entrapment into two different gelatin membranes or by covalent attachment of the enzyme on two nylon membranes, differently preactivated. Results have shown that the field affects the inactivation rate of the soluble enzyme, while no effects are observed with insoluble derivatives. Since in vivo enzymes are immobilised into the biomembrane bilayer or entrapped into the cytoplasmic mixture, one might speculate that our experimental conditions do not reflect the catalytic activity of the enzymes in vivo.

Machine Enhanced Logic

AI evidence extraction

Structured fields below were generated from the paper’s abstract and metadata to make the evidence easier to inspect. Automated extraction may be incomplete or incorrect; verify important details against the original paper.

Study typein_vitro

Effect directionmixed

PopulationNot supplied

Sample sizeNot supplied

ExposureELF · studied as a function of time

Evidence strengthLowConfidence: 78% · Peer reviewed: yes

Main findings

Outcomes measured

Activity/inactivation rate of soluble horseradish peroxidase
Activity/inactivation rate of insoluble (immobilized) horseradish peroxidase derivatives (gelatin membranes; nylon membranes)

Limitations

In vitro enzyme system; authors note conditions may not reflect in vivo catalytic activity
No sample size or quantitative effect estimates reported in the abstract

View raw extracted JSON

{
  "study_type": "in_vitro",
  "exposure": {
    "band": "ELF",
    "source": "laboratory magnetic field exposure",
    "frequency_mhz": null,
    "sar_wkg": null,
    "duration": "studied as a function of time"
  },
  "population": null,
  "sample_size": null,
  "outcomes": [
    "Activity/inactivation rate of soluble horseradish peroxidase",
    "Activity/inactivation rate of insoluble (immobilized) horseradish peroxidase derivatives (gelatin membranes; nylon membranes)"
  ],
  "main_findings": "An ELF magnetic field (50 Hz, 1 mT) affected the inactivation rate of soluble horseradish peroxidase, while no effects were observed for insoluble (immobilized) enzyme derivatives prepared in gelatin or nylon membranes.",
  "effect_direction": "mixed",
  "limitations": [
    "In vitro enzyme system; authors note conditions may not reflect in vivo catalytic activity",
    "No sample size or quantitative effect estimates reported in the abstract"
  ],
  "evidence_strength": "low",
  "confidence": 0.78,
  "peer_reviewed_likely": "yes",
  "keywords": [
    "ELF",
    "magnetic field",
    "50 Hz",
    "1 mT",
    "horseradish peroxidase",
    "enzyme activity",
    "enzyme inactivation",
    "immobilized enzyme",
    "gelatin membrane",
    "nylon membrane",
    "in vitro"
  ],
  "suggested_hubs": []
}

AI can be wrong. Always verify against the paper.

AI-extracted fields are generated from the abstract and metadata and may be incomplete or incorrect. This material is for informational purposes and is not medical advice.

Identifiers and source

DOI: 10.1002/bem.10119
PubMed: 12955749

Read the original paper →

Related papers

← Back to the Research Library