15 Hz square wave magnetic fields affects voltage-gated sodium & potassium channels in neurons

2016 · Harm · Low evidence

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HarmLow evidencePaper 1373

Exposure to a 15 Hz, 1 mT square-wave magnetic field depressed sodium and potassium channel current densities and altered activation kinetics. Inactivation properties of INa and IA were also altered.

Study snapshot

Publicationmanual · 2016

Study typeanimal

Reported directionHarm

Evidence ratingLow

Abstract

15 Hz square wave magnetic fields affects voltage-gated sodium & potassium channels in neurons Zheng Y, Dou JR, Gao Y, Dong L, Li G. Effects of 15 Hz square wave magnetic fields on the voltage-gated sodium and potassium channels in prefrontal cortex pyramidal neurons. Int J Radiat Biol. 2016 Dec 7:1-7. Abstract PURPOSE: Although magnetic fields have significant effects on neurons, little is known about the mechanisms behind their effects. The present study aimed to measure the effects of magnetic fields on ion channels in cortical pyramidal neurons. MATERIALS AND METHODS: Cortical pyramidal neurons of Kunming mice were isolated and then subjected to 15 Hz, 1 mT square wave (duty ratio 50%) magnetic fields stimulation. Sodium currents (INa), transient potassium currents (IA) and delayed rectifier potassium currents (IK) were recorded by whole-cell patch clamp method. RESULTS: We found that magnetic field exposure depressed channel current densities, and altered the activation kinetics of sodium and potassium channels. The inactivation properties of INa and IA were also altered. CONCLUSION: Magnetic field exposure alters ion channel function in neurons. It is likely that the structures of sodium and potassium channels were influenced by the applied field. Sialic acid, which is an important component of the channels, could be the molecule responsible for the reported results. ncbi.nlm.nih.gov

Machine Enhanced Logic

AI evidence extraction

Structured fields below were generated from the paper’s abstract and metadata to make the evidence easier to inspect. Automated extraction may be incomplete or incorrect; verify important details against the original paper.

Study typeanimal

Effect directionharm

PopulationIsolated prefrontal cortex pyramidal neurons from Kunming mice

Sample sizeNot supplied

ExposureELF · 0.015 MHz

Evidence strengthLowConfidence: 74% · Peer reviewed: yes

Main findings

Exposure to a 15 Hz, 1 mT square-wave magnetic field depressed sodium and potassium channel current densities and altered activation kinetics. Inactivation properties of INa and IA were also altered.

Outcomes measured

Voltage-gated sodium channel currents (INa)
Transient potassium channel currents (IA)
Delayed rectifier potassium channel currents (IK)
Channel current density
Activation kinetics (sodium and potassium channels)
Inactivation properties (INa and IA)

Limitations

Exposure duration not reported in abstract
Sample size not reported in abstract
Ex vivo/in vitro preparation (isolated neurons) may limit generalizability to intact organisms
Mechanistic attribution (e.g., sialic acid involvement) is speculative in the abstract

View raw extracted JSON

{
  "study_type": "animal",
  "exposure": {
    "band": "ELF",
    "source": null,
    "frequency_mhz": 0.015,
    "sar_wkg": null,
    "duration": null
  },
  "population": "Isolated prefrontal cortex pyramidal neurons from Kunming mice",
  "sample_size": null,
  "outcomes": [
    "Voltage-gated sodium channel currents (INa)",
    "Transient potassium channel currents (IA)",
    "Delayed rectifier potassium channel currents (IK)",
    "Channel current density",
    "Activation kinetics (sodium and potassium channels)",
    "Inactivation properties (INa and IA)"
  ],
  "main_findings": "Exposure to a 15 Hz, 1 mT square-wave magnetic field depressed sodium and potassium channel current densities and altered activation kinetics. Inactivation properties of INa and IA were also altered.",
  "effect_direction": "harm",
  "limitations": [
    "Exposure duration not reported in abstract",
    "Sample size not reported in abstract",
    "Ex vivo/in vitro preparation (isolated neurons) may limit generalizability to intact organisms",
    "Mechanistic attribution (e.g., sialic acid involvement) is speculative in the abstract"
  ],
  "evidence_strength": "low",
  "confidence": 0.74,
  "peer_reviewed_likely": "yes",
  "keywords": [
    "15 Hz",
    "square wave",
    "magnetic field",
    "1 mT",
    "ELF",
    "neurons",
    "prefrontal cortex",
    "pyramidal neurons",
    "Kunming mice",
    "patch clamp",
    "voltage-gated sodium channel",
    "voltage-gated potassium channel",
    "INa",
    "IA",
    "IK"
  ],
  "suggested_hubs": [
    {
      "slug": "elr-elf-mechanisms",
      "weight": 0.78,
      "reason": "ELF (15 Hz) magnetic-field exposure with electrophysiological effects on neuronal ion channels."
    }
  ]
}

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AI-extracted fields are generated from the abstract and metadata and may be incomplete or incorrect. This material is for informational purposes and is not medical advice.

Identifiers and source

DOI: Not supplied
PubMed: Not supplied

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