Phospholipase C-dependent phosphoinositide breakdown induced by ELF-EMF in Peganum harmala calli.

Biochimie · 2004 · Mixed · Low evidence

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MixedLow evidencePaper 5224

Exposure of Peganum harmala callus cells to a 50 Hz, 1 gauss EMF for 10 minutes was associated with a statistically significant decrease in PtdIns 4,5-P2 concentrations and changes in the involvement of constituting fatty acids in the breakdown. Use of PI-PLC…

Study snapshot

PublicationBiochimie · 2004

Study typeother

Reported directionMixed

Evidence ratingLow

Abstract

With the aim of examining the response of plant cells to extremely low frequency (ELF) electromagnetic fields (EMF), we investigated the behaviour of the phosphatidylinositol 4,5 bisphosphate (PtdIns 4,5-P(2)) molecule (the precursor of the phosphoinositide signal transduction cascade) by exposing callus cells from Peganum harmala to 50 Hz, 1 gauss EMF for 10 min and by examining the level and the fatty acid composition of PtdIns 4,5-P(2) after the exposure. Our results evidenced a statistically significant decrease in PtdIns 4,5-P(2) concentrations and a different involvement of the constituting fatty acids in the induced breakdown. The manipulation of the lipid-based signalling pathway by phosphoinositide-phospholipase C (PI-PLC) inhibitors (i.e., neomycin, U-73122 and ET-18-OCH(3)) seems to support the hypothesis that, as in animals, also in plants, the cell membrane is the primary impact site of ELF electromagnetic stimulus and that this interaction could probably involve the activation of PI signal transduction pathway including a heterotrimeric G protein.

Machine Enhanced Logic

AI evidence extraction

Structured fields below were generated from the paper’s abstract and metadata to make the evidence easier to inspect. Automated extraction may be incomplete or incorrect; verify important details against the original paper.

Study typeother

Effect directionmixed

PopulationPeganum harmala callus cells (plant cells)

Sample sizeNot supplied

ExposureELF · 0.05 MHz · 10 min

Evidence strengthLowConfidence: 74% · Peer reviewed: yes

Main findings

Outcomes measured

Phosphatidylinositol 4,5-bisphosphate (PtdIns 4,5-P2) concentration after exposure
Fatty acid composition of PtdIns 4,5-P2 after exposure
Evidence consistent with PI-PLC involvement using inhibitors (neomycin, U-73122, ET-18-OCH3)

Limitations

Sample size not reported in abstract
Study is in plant callus cells; relevance to human health not addressed
Exposure metric reported as 1 gauss; no dosimetry beyond field strength and duration provided
Mechanistic interpretation (e.g., membrane as primary impact site, G protein involvement) is presented as a hypothesis

View raw extracted JSON

{
  "study_type": "other",
  "exposure": {
    "band": "ELF",
    "source": "other",
    "frequency_mhz": 0.05,
    "sar_wkg": null,
    "duration": "10 min"
  },
  "population": "Peganum harmala callus cells (plant cells)",
  "sample_size": null,
  "outcomes": [
    "Phosphatidylinositol 4,5-bisphosphate (PtdIns 4,5-P2) concentration after exposure",
    "Fatty acid composition of PtdIns 4,5-P2 after exposure",
    "Evidence consistent with PI-PLC involvement using inhibitors (neomycin, U-73122, ET-18-OCH3)"
  ],
  "main_findings": "Exposure of Peganum harmala callus cells to a 50 Hz, 1 gauss EMF for 10 minutes was associated with a statistically significant decrease in PtdIns 4,5-P2 concentrations and changes in the involvement of constituting fatty acids in the breakdown. Use of PI-PLC inhibitors was reported to support involvement of a phosphoinositide/PI-PLC signaling pathway.",
  "effect_direction": "mixed",
  "limitations": [
    "Sample size not reported in abstract",
    "Study is in plant callus cells; relevance to human health not addressed",
    "Exposure metric reported as 1 gauss; no dosimetry beyond field strength and duration provided",
    "Mechanistic interpretation (e.g., membrane as primary impact site, G protein involvement) is presented as a hypothesis"
  ],
  "evidence_strength": "low",
  "confidence": 0.74,
  "peer_reviewed_likely": "yes",
  "keywords": [
    "ELF-EMF",
    "50 Hz",
    "1 gauss",
    "Peganum harmala",
    "callus cells",
    "phosphoinositides",
    "PtdIns 4,5-P2",
    "phospholipase C",
    "PI-PLC inhibitors",
    "signal transduction",
    "cell membrane"
  ],
  "suggested_hubs": []
}

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AI-extracted fields are generated from the abstract and metadata and may be incomplete or incorrect. This material is for informational purposes and is not medical advice.

Identifiers and source

DOI: 10.1016/j.biochi.2004.02.001
PubMed: 15194239

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