Effect of extremely low frequency electromagnetic fields (ELF-EMF) on Kaposi's sarcoma-associated herpes virus in BCBL-1 cells.

Bioelectromagnetics · 2006 · Mixed · Low evidence

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MixedLow evidencePaper 5148

Study snapshot

PublicationBioelectromagnetics · 2006

Study typein_vitro

Reported directionMixed

Evidence ratingLow

Abstract

Association between extremely low frequency electromagnetic fields (ELF-EMF) and human cancers is controversial, and few studies have been conducted on their influence on oncogenic viruses. We studied the effects of 1 mT, 50 Hz sine waves, applied for 24-72 h, on Kaposi's sarcoma (KS)-associated herpesvirus (KSHV or HHV-8) in BCBL-1, a latently infected primary effusion lymphoma (PEL) cell line. ELF-EMF exposure did not affect the growth and viability of BCBL-1 cells, either stimulated or not with TPA. The total amount of KSHV DNA detected in ELF-EMF exposed cultures not stimulated with TPA did not differ from that of the unexposed controls (P = ns). However, in the presence of TPA stimulation, total KSHV DNA content was found higher in ELF-EMF exposed than in control BCBL-1 cultures (P = .024) at 72 h exposure, but not earlier. Viral DNA increase significantly correlated with increased mean fluorescence intensity/cell for the lytic antigen gp K8.1A/B (P < .01), but not with percentage of gp K8.1A/B-positive cells or of cells containing virions. Viral progeny produced under ELF-EMF exposure consisted mainly of defective viral particles.

Machine Enhanced Logic

AI evidence extraction

Structured fields below were generated from the paper’s abstract and metadata to make the evidence easier to inspect. Automated extraction may be incomplete or incorrect; verify important details against the original paper.

Study typein_vitro

Effect directionmixed

PopulationBCBL-1 latently infected primary effusion lymphoma (PEL) cell line

Sample sizeNot supplied

ExposureELF · 0.05 MHz · 24–72 h

Evidence strengthLowConfidence: 78% · Peer reviewed: yes

Main findings

Exposure to 1 mT, 50 Hz sine waves for 24–72 h did not affect BCBL-1 cell growth or viability (with or without TPA). Without TPA, total KSHV DNA did not differ between exposed and control cultures. With TPA stimulation, total KSHV DNA was higher in exposed vs control cultures at 72 h (P = .024) but not earlier, and this increase correlated with increased gp K8.1A/B mean fluorescence intensity per cell (P < .01); viral progeny produced under exposure consisted mainly of defective particles.

Outcomes measured

BCBL-1 cell growth
BCBL-1 cell viability
Total KSHV (HHV-8) DNA content
Lytic antigen gp K8.1A/B mean fluorescence intensity per cell
Percentage of gp K8.1A/B-positive cells
Percentage of cells containing virions
Viral progeny particle quality (defective particles)

Limitations

Sample size not reported in abstract
Findings are from an in vitro cell line model; human health implications not directly assessed
Effect on viral DNA observed only under TPA stimulation and only at 72 h (not earlier)

View raw extracted JSON

{
  "study_type": "in_vitro",
  "exposure": {
    "band": "ELF",
    "source": null,
    "frequency_mhz": 0.05,
    "sar_wkg": null,
    "duration": "24–72 h"
  },
  "population": "BCBL-1 latently infected primary effusion lymphoma (PEL) cell line",
  "sample_size": null,
  "outcomes": [
    "BCBL-1 cell growth",
    "BCBL-1 cell viability",
    "Total KSHV (HHV-8) DNA content",
    "Lytic antigen gp K8.1A/B mean fluorescence intensity per cell",
    "Percentage of gp K8.1A/B-positive cells",
    "Percentage of cells containing virions",
    "Viral progeny particle quality (defective particles)"
  ],
  "main_findings": "Exposure to 1 mT, 50 Hz sine waves for 24–72 h did not affect BCBL-1 cell growth or viability (with or without TPA). Without TPA, total KSHV DNA did not differ between exposed and control cultures. With TPA stimulation, total KSHV DNA was higher in exposed vs control cultures at 72 h (P = .024) but not earlier, and this increase correlated with increased gp K8.1A/B mean fluorescence intensity per cell (P < .01); viral progeny produced under exposure consisted mainly of defective particles.",
  "effect_direction": "mixed",
  "limitations": [
    "Sample size not reported in abstract",
    "Findings are from an in vitro cell line model; human health implications not directly assessed",
    "Effect on viral DNA observed only under TPA stimulation and only at 72 h (not earlier)"
  ],
  "evidence_strength": "low",
  "confidence": 0.78,
  "peer_reviewed_likely": "yes",
  "keywords": [
    "extremely low frequency",
    "ELF-EMF",
    "50 Hz",
    "1 mT",
    "Kaposi's sarcoma-associated herpesvirus",
    "KSHV",
    "HHV-8",
    "BCBL-1",
    "primary effusion lymphoma",
    "TPA",
    "lytic antigen",
    "gp K8.1A/B",
    "viral DNA",
    "defective viral particles"
  ],
  "suggested_hubs": []
}

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AI-extracted fields are generated from the abstract and metadata and may be incomplete or incorrect. This material is for informational purposes and is not medical advice.

Identifiers and source

DOI: 10.1002/bem.20198
PubMed: 16342195

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