Effect of ELF-EMF on number of apoptotic cells; correlation with reactive oxygen species and HSP.

Acta biologica Hungarica · 2010 · Mixed · Low evidence

← Research stream

MixedLow evidencePaper 4377

ELF-EMF exposure (1 mT, 50 Hz, 3 h) decreased the number of apoptotic cells and slightly increased viability in K562 cells without oxidative stress. In H2O2-treated cells, ELF-EMF increased apoptotic cell number and increased hsp70 and ROS levels.

Study snapshot

PublicationActa biologica Hungarica · 2010

Study typein_vitro

Reported directionMixed

Evidence ratingLow

Abstract

It is by now accepted that extremely low frequency electromagnetic fields ELF-EMF (0-300 Hz) affect biological systems although the mechanism has not been elucidated yet. In this study the effect of ELFEMF on the number of apoptotic cells of K562 human leukemia cell line induced or not with oxidative stress and the correlation with heat-shock protein 70 (hsp70) levels was investigated. One sample was treated with H 2 O 2 while the other was left untreated. ELF-EMF (1 mT, 50 Hz) was applied for 3 hours. ELF-EMF alone caused a decrease in the number of apoptotic cells and a slight increase in viability. However, it increased the number of apoptotic cells. In cells treated with H 2 O 2 . hsp70 and reactive oxygen species (ROS) levels were increased by ELF-EMF. These results show that the effect of ELF-EMF on biological systems depends on the status of the cell: while in cells not exposed to oxidative stress it is able to decrease the number of apoptotic cells by inducing an increase in hsp levels, it increases the number of apoptotic cells in oxidative stress-induced cells.

Machine Enhanced Logic

AI evidence extraction

Structured fields below were generated from the paper’s abstract and metadata to make the evidence easier to inspect. Automated extraction may be incomplete or incorrect; verify important details against the original paper.

Study typein_vitro

Effect directionmixed

PopulationK562 human leukemia cell line

Sample sizeNot supplied

ExposureELF · 0.05 MHz · 3 hours

Evidence strengthLowConfidence: 78% · Peer reviewed: yes

Main findings

Outcomes measured

Apoptotic cell number
Cell viability
Reactive oxygen species (ROS) levels
Heat-shock protein 70 (hsp70) levels

Limitations

In vitro cell-line study (K562), limiting generalizability to humans
Sample size not reported in abstract
Only one field strength/frequency and exposure duration reported (1 mT, 50 Hz, 3 h)
Oxidative stress model based on H2O2 treatment; may not reflect in vivo conditions

View raw extracted JSON

{
  "study_type": "in_vitro",
  "exposure": {
    "band": "ELF",
    "source": null,
    "frequency_mhz": 0.05,
    "sar_wkg": null,
    "duration": "3 hours"
  },
  "population": "K562 human leukemia cell line",
  "sample_size": null,
  "outcomes": [
    "Apoptotic cell number",
    "Cell viability",
    "Reactive oxygen species (ROS) levels",
    "Heat-shock protein 70 (hsp70) levels"
  ],
  "main_findings": "ELF-EMF exposure (1 mT, 50 Hz, 3 h) decreased the number of apoptotic cells and slightly increased viability in K562 cells without oxidative stress. In H2O2-treated cells, ELF-EMF increased apoptotic cell number and increased hsp70 and ROS levels.",
  "effect_direction": "mixed",
  "limitations": [
    "In vitro cell-line study (K562), limiting generalizability to humans",
    "Sample size not reported in abstract",
    "Only one field strength/frequency and exposure duration reported (1 mT, 50 Hz, 3 h)",
    "Oxidative stress model based on H2O2 treatment; may not reflect in vivo conditions"
  ],
  "evidence_strength": "low",
  "confidence": 0.78,
  "peer_reviewed_likely": "yes",
  "keywords": [
    "ELF-EMF",
    "extremely low frequency",
    "50 Hz",
    "1 mT",
    "apoptosis",
    "K562",
    "oxidative stress",
    "H2O2",
    "reactive oxygen species",
    "ROS",
    "HSP70",
    "heat-shock protein"
  ],
  "suggested_hubs": []
}

AI can be wrong. Always verify against the paper.

AI-extracted fields are generated from the abstract and metadata and may be incomplete or incorrect. This material is for informational purposes and is not medical advice.

Identifiers and source

DOI: 10.1556/abiol.61.2010.2.4
PubMed: 20519170

Read the original paper →

Related papers

← Back to the Research Library