Proteomic response of Schizosaccharomyces pombe to static and oscillating extremely low-frequency electromagnetic fields.

Proteomics · 2006 · No effect · Low evidence

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No effectLow evidencePaper 5109

Study snapshot

PublicationProteomics · 2006

Study typein_vitro

Reported directionNo effect

Evidence ratingLow

Abstract

There is considerable public concern regarding the health effects of exposure to low-frequency electromagnetic fields. In addition, the association between exposure and disease incidence or the possible biological effects of exposure are unclear. Using 2D-DIGE and MS in a blind study, we have investigated the effects of static and oscillating extremely low-frequency electromagnetic fields (ELF EMFs) on the proteomes of wild type Schizosaccharomyces pombe and a Sty1p deletion mutant which displays increased sensitivity to a variety of cellular stresses. Whilst this study identifies a number of protein isoforms that display significant differential expression across experimental conditions, there was no correlation between their patterns of expression and the ELF EMF exposure regimen. We conclude that there are no significant effects of either static or oscillating EMF on the yeast proteome at the sensitivity afforded by 2D-DIGE. We hypothesise that the proteins identified must be sensitive to subtle changes in culture and/or handling conditions, and that the identification of these proteins in other proteomic studies should be treated with some caution when the results of such studies are interpreted in a biological context.

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AI evidence extraction

Structured fields below were generated from the paper’s abstract and metadata to make the evidence easier to inspect. Automated extraction may be incomplete or incorrect; verify important details against the original paper.

Study typein_vitro

Effect directionno_effect

PopulationSchizosaccharomyces pombe (wild type) and Sty1p deletion mutant yeast

Sample sizeNot supplied

ExposureELF

Evidence strengthLowConfidence: 74% · Peer reviewed: yes

Main findings

In a blind 2D-DIGE/MS study of wild type and Sty1p deletion mutant Schizosaccharomyces pombe, some protein isoforms showed significant differential expression across experimental conditions, but expression patterns did not correlate with the ELF EMF exposure regimen. The authors conclude there were no significant effects of static or oscillating ELF EMF on the yeast proteome at the sensitivity of 2D-DIGE.

Outcomes measured

Proteome/protein isoform differential expression (2D-DIGE, MS)

Limitations

Exposure parameters (e.g., field strength, frequency, duration) not reported in the abstract
Sensitivity limited to what can be detected by 2D-DIGE
Differentially expressed proteins may reflect subtle culture/handling differences rather than exposure effects

View raw extracted JSON

{
  "study_type": "in_vitro",
  "exposure": {
    "band": "ELF",
    "source": null,
    "frequency_mhz": null,
    "sar_wkg": null,
    "duration": null
  },
  "population": "Schizosaccharomyces pombe (wild type) and Sty1p deletion mutant yeast",
  "sample_size": null,
  "outcomes": [
    "Proteome/protein isoform differential expression (2D-DIGE, MS)"
  ],
  "main_findings": "In a blind 2D-DIGE/MS study of wild type and Sty1p deletion mutant Schizosaccharomyces pombe, some protein isoforms showed significant differential expression across experimental conditions, but expression patterns did not correlate with the ELF EMF exposure regimen. The authors conclude there were no significant effects of static or oscillating ELF EMF on the yeast proteome at the sensitivity of 2D-DIGE.",
  "effect_direction": "no_effect",
  "limitations": [
    "Exposure parameters (e.g., field strength, frequency, duration) not reported in the abstract",
    "Sensitivity limited to what can be detected by 2D-DIGE",
    "Differentially expressed proteins may reflect subtle culture/handling differences rather than exposure effects"
  ],
  "evidence_strength": "low",
  "confidence": 0.74,
  "peer_reviewed_likely": "yes",
  "keywords": [
    "extremely low-frequency",
    "ELF EMF",
    "static electromagnetic field",
    "oscillating electromagnetic field",
    "proteomics",
    "2D-DIGE",
    "mass spectrometry",
    "Schizosaccharomyces pombe",
    "Sty1p deletion mutant",
    "stress response"
  ],
  "suggested_hubs": []
}

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AI-extracted fields are generated from the abstract and metadata and may be incomplete or incorrect. This material is for informational purposes and is not medical advice.

Identifiers and source

DOI: 10.1002/pmic.200500861
PubMed: 16897687

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