RF Safe

Comparative study of cell cycle kinetics and induction of apoptosis or necrosis after exposure of human Mono Mac 6 cells to radiofrequency radiation.

Radiation research · 2006 · No effect · Low evidence

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No effectLow evidencePaper 5106

After exposure to a 1,800 MHz GSM-DTX signal at 2 W/kg SAR for 12 h, no statistically significant differences were detected in apoptosis, necrosis, cell cycle kinetics, or BrdU uptake compared with sham or incubator controls. Positive controls (gliotoxin and …

Study snapshot

PublicationRadiation research · 2006
Study typein_vitro
Reported directionNo effect
Evidence ratingLow

Abstract

The possible harmful effects of radiofrequency electromagnetic fields (RF EMFs) are controversial. We have used human Mono Mac 6 cells to investigate the influence of RF EMFs in vitro on cell cycle alterations and BrdU uptake, as well as the induction of apoptosis and necrosis in human Mono Mac 6 cells, using flow cytometry after exposure to a 1,800 MHz, 2 W/kg specific absorption rate (SAR), GSM-DTX signal for 12 h. No statistically significant differences in the induction of apoptosis or necrosis, cell cycle kinetics, or BrdU uptake were detected after RF EMF exposure compared to sham or incubator controls. However, in the positive control cells treated with gliotoxin and PMA (phorbol 12 myristate-13 acetate), a significant increase in apoptotic and necrotic cells was seen. Cell cycle analysis or BrdU incorporation for 72 h showed no differences between RF EMF- or sham-exposed cells, whereas PMA treatment induced a significant accumulation of cells in G(0)/G(1)-phase and a reduction in S-phase cells. RF EMF radiation did not induce cell cycle alterations or changes in BrdU incorporation or induce apoptosis and necrosis in Mono Mac 6 cells under the exposure conditions used.

Machine Enhanced Logic

AI evidence extraction

Structured fields below were generated from the paper’s abstract and metadata to make the evidence easier to inspect. Automated extraction may be incomplete or incorrect; verify important details against the original paper.

Study typein_vitro
Effect directionno_effect
PopulationHuman Mono Mac 6 cells (in vitro)
Sample sizeNot supplied
ExposureRF · 1800 MHz · 2 W/kg · 12 h (cell cycle/BrdU also assessed up to 72 h)
Evidence strengthLowConfidence: 86% · Peer reviewed: yes

Main findings

After exposure to a 1,800 MHz GSM-DTX signal at 2 W/kg SAR for 12 h, no statistically significant differences were detected in apoptosis, necrosis, cell cycle kinetics, or BrdU uptake compared with sham or incubator controls. Positive controls (gliotoxin and PMA) showed significant increases in apoptotic/necrotic cells and PMA altered cell cycle distribution.

Outcomes measured

  • Apoptosis
  • Necrosis
  • Cell cycle kinetics/alterations
  • BrdU uptake/incorporation

Limitations

  • Sample size not reported in abstract
  • Findings limited to one human cell line and in vitro conditions
  • Single exposure condition reported (1,800 MHz, 2 W/kg SAR, 12 h)
View raw extracted JSON
{
  "study_type": "in_vitro",
  "exposure": {
    "band": "RF",
    "source": null,
    "frequency_mhz": 1800,
    "sar_wkg": 2,
    "duration": "12 h (cell cycle/BrdU also assessed up to 72 h)"
  },
  "population": "Human Mono Mac 6 cells (in vitro)",
  "sample_size": null,
  "outcomes": [
    "Apoptosis",
    "Necrosis",
    "Cell cycle kinetics/alterations",
    "BrdU uptake/incorporation"
  ],
  "main_findings": "After exposure to a 1,800 MHz GSM-DTX signal at 2 W/kg SAR for 12 h, no statistically significant differences were detected in apoptosis, necrosis, cell cycle kinetics, or BrdU uptake compared with sham or incubator controls. Positive controls (gliotoxin and PMA) showed significant increases in apoptotic/necrotic cells and PMA altered cell cycle distribution.",
  "effect_direction": "no_effect",
  "limitations": [
    "Sample size not reported in abstract",
    "Findings limited to one human cell line and in vitro conditions",
    "Single exposure condition reported (1,800 MHz, 2 W/kg SAR, 12 h)"
  ],
  "evidence_strength": "low",
  "confidence": 0.86,
  "peer_reviewed_likely": "yes",
  "keywords": [
    "radiofrequency",
    "RF EMF",
    "GSM-DTX",
    "1800 MHz",
    "SAR 2 W/kg",
    "Mono Mac 6",
    "apoptosis",
    "necrosis",
    "cell cycle",
    "BrdU",
    "flow cytometry",
    "in vitro"
  ],
  "suggested_hubs": []
}
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AI-extracted fields are generated from the abstract and metadata and may be incomplete or incorrect. This material is for informational purposes and is not medical advice.

Identifiers and source

DOI: 10.1667/rr3601.1
PubMed: 16953672

Read the original paper →

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