The effect of electromagnetic radiation (550-850 nm) on 1-lactate dehydrogenase kinetics.

International journal of radiation biology · 2007 · Benefit · Low evidence

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BenefitLow evidencePaper 5060

LDH enzyme activity was compared before and after monochromatic visible-light exposures in a computationally predicted range. Activity was reported to be selectively increased only at wavelengths 595 nm and 828 nm, which were within the range predicted by the…

Study snapshot

PublicationInternational journal of radiation biology · 2007

Study typein_vitro

Reported directionBenefit

Evidence ratingLow

Abstract

PURPOSE: This work is based on our earlier research of the Resonant Recognition Model (RRM), where we have proposed that protein activation is electromagnetic in its nature. In this study we investigated experimentally the possibility of modulating the protein activity by the electromagnetic radiation of the specific frequency. The concept is studied here by applying a visible light radiation to example of 1-Lactate Dehydrogenase enzyme (LDH). MATERIALS AND METHODS: The selected LDH example is radiated by monochromatic visible light in a frequency range predicted computationally by the RRM. The kinetics of the irradiated LDH is measured by continuous monitoring of the NADH absorption at 340 nm. RESULTS: A comparative analysis of the LDH enzyme activity before and after the electromagnetic field (EMF) exposures is performed. It was found that the LDH activity is selectively increased only by the radiation at the particular wavelengths of 595 nm and 828 nm. These experimentally determined wavelengths of the applied EMF are within the range predicted by the RRM. CONCLUSIONS: Results reveal the LDH activity was modulated by the EMF exposures at the computationally predicted frequencies. The RRM concept presented provides new insights into proteins susceptibility to perturbation by electromagnetic radiation and possibility to program, predict, design and modify proteins and their bioactivity.

Machine Enhanced Logic

AI evidence extraction

Structured fields below were generated from the paper’s abstract and metadata to make the evidence easier to inspect. Automated extraction may be incomplete or incorrect; verify important details against the original paper.

Study typein_vitro

Effect directionbenefit

PopulationNot supplied

Sample sizeNot supplied

Exposurevisible light

Evidence strengthLowConfidence: 74% · Peer reviewed: yes

Main findings

Outcomes measured

1-Lactate dehydrogenase (LDH) enzyme activity/kinetics (via NADH absorption at 340 nm)

Limitations

No sample size or number of replicates reported in the abstract.
Exposure parameters beyond wavelength (e.g., intensity/irradiance, duration) are not described in the abstract.
Study is an in vitro enzyme experiment; generalizability to organisms/health outcomes is not addressed in the abstract.

View raw extracted JSON

{
  "study_type": "in_vitro",
  "exposure": {
    "band": "visible light",
    "source": null,
    "frequency_mhz": null,
    "sar_wkg": null,
    "duration": null
  },
  "population": null,
  "sample_size": null,
  "outcomes": [
    "1-Lactate dehydrogenase (LDH) enzyme activity/kinetics (via NADH absorption at 340 nm)"
  ],
  "main_findings": "LDH enzyme activity was compared before and after monochromatic visible-light exposures in a computationally predicted range. Activity was reported to be selectively increased only at wavelengths 595 nm and 828 nm, which were within the range predicted by the Resonant Recognition Model (RRM).",
  "effect_direction": "benefit",
  "limitations": [
    "No sample size or number of replicates reported in the abstract.",
    "Exposure parameters beyond wavelength (e.g., intensity/irradiance, duration) are not described in the abstract.",
    "Study is an in vitro enzyme experiment; generalizability to organisms/health outcomes is not addressed in the abstract."
  ],
  "evidence_strength": "low",
  "confidence": 0.74,
  "peer_reviewed_likely": "yes",
  "keywords": [
    "electromagnetic radiation",
    "visible light",
    "monochromatic",
    "595 nm",
    "828 nm",
    "lactate dehydrogenase",
    "LDH",
    "enzyme kinetics",
    "NADH absorption",
    "Resonant Recognition Model",
    "protein activation"
  ],
  "suggested_hubs": []
}

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AI-extracted fields are generated from the abstract and metadata and may be incomplete or incorrect. This material is for informational purposes and is not medical advice.

Identifiers and source

DOI: 10.1080/09553000701227565
PubMed: 17575949

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