RF Safe

[Surface markers and functions of human dendritic cells exposed to mobile phone 1800 MHz electromagnetic fields].

Zhejiang da xue xue bao. Yi xue ban = Journal of Zhejiang University. Medical sciences · 2008 · Mixed · Low evidence

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MixedLow evidencePaper 5027

Compared with sham, exposure for 1 h, 12 h, or 24 h decreased HLA-DR, CD80, CD86, and CD40 (CD11c not decreased). DC allo-MLR stimulation ability was significantly decreased (P<0.05), especially after 24 h, while IL-12p70 and TNF-alpha secretion levels were u…

Study snapshot

PublicationZhejiang da xue xue bao. Yi xue ban = Journal of Zhejiang University. Medical sciences · 2008
Study typein_vitro
Reported directionMixed
Evidence ratingLow

Abstract

OBJECTIVE: To investigate the effects of mobile phone 1800 MHz electromagnetic fields (EMF) on the surface markers and the functions of human dendritic cells (DC). METHODS: Human DCs were exposed to intermittent 5 min on/10 min off EMF with specific absorption rates (SAR) 4 W/kg for 0 h, 1 h, 12 h or 24 h, respectively. FACS analysis was used to detect the positive percentage of DC surface markers including HLA-DR and co-stimulatory molecules such as CD80, CD86, CD40 and CD11c. CCK-8 kit was adopted to examine the function of allo-mixed lymphocyte reaction (allo-MLR) of DC, and enzyme linked immunosorbent assay (ELISA) to identify the levels of IL-12p70 and TNF-alpha secreted by DC. RESULT: Compared with the sham radiation group, after exposure to the electromagnetic fields for 1 h, 12 h, or 24 h, HLA-DR, CD80,CD86 and CD40 were all declined except CD11c. The ability of DC allo-MLR in each exposure group was decreased significantly (P<0.05), especially in the 24 h exposure group. However, the secreted levels of IL-12p70 and TNF-alpha of DC in each exposure group remained no changed. CONCLUSION: The study showed that EMF exposure could down-regulate the surface molecules and stimulation ability of human DC.

Machine Enhanced Logic

AI evidence extraction

Structured fields below were generated from the paper’s abstract and metadata to make the evidence easier to inspect. Automated extraction may be incomplete or incorrect; verify important details against the original paper.

Study typein_vitro
Effect directionmixed
PopulationHuman dendritic cells (DC)
Sample sizeNot supplied
ExposureRF · 1800 MHz · 4 W/kg · 0 h, 1 h, 12 h, or 24 h (intermittent 5 min on/10 min off)
Evidence strengthLowConfidence: 78% · Peer reviewed: yes

Main findings

Compared with sham, exposure for 1 h, 12 h, or 24 h decreased HLA-DR, CD80, CD86, and CD40 (CD11c not decreased). DC allo-MLR stimulation ability was significantly decreased (P<0.05), especially after 24 h, while IL-12p70 and TNF-alpha secretion levels were unchanged.

Outcomes measured

  • DC surface marker expression (HLA-DR, CD80, CD86, CD40, CD11c)
  • Allo-mixed lymphocyte reaction (allo-MLR) stimulation ability
  • Cytokine secretion (IL-12p70, TNF-alpha)

Limitations

  • Sample size not reported in abstract
  • In vitro study (human cells), not a clinical/epidemiologic outcome
  • Only one frequency (1800 MHz) and one SAR level (4 W/kg) reported
  • Intermittent exposure pattern may limit generalizability to other exposure scenarios
View raw extracted JSON
{
  "study_type": "in_vitro",
  "exposure": {
    "band": "RF",
    "source": "mobile phone",
    "frequency_mhz": 1800,
    "sar_wkg": 4,
    "duration": "0 h, 1 h, 12 h, or 24 h (intermittent 5 min on/10 min off)"
  },
  "population": "Human dendritic cells (DC)",
  "sample_size": null,
  "outcomes": [
    "DC surface marker expression (HLA-DR, CD80, CD86, CD40, CD11c)",
    "Allo-mixed lymphocyte reaction (allo-MLR) stimulation ability",
    "Cytokine secretion (IL-12p70, TNF-alpha)"
  ],
  "main_findings": "Compared with sham, exposure for 1 h, 12 h, or 24 h decreased HLA-DR, CD80, CD86, and CD40 (CD11c not decreased). DC allo-MLR stimulation ability was significantly decreased (P<0.05), especially after 24 h, while IL-12p70 and TNF-alpha secretion levels were unchanged.",
  "effect_direction": "mixed",
  "limitations": [
    "Sample size not reported in abstract",
    "In vitro study (human cells), not a clinical/epidemiologic outcome",
    "Only one frequency (1800 MHz) and one SAR level (4 W/kg) reported",
    "Intermittent exposure pattern may limit generalizability to other exposure scenarios"
  ],
  "evidence_strength": "low",
  "confidence": 0.78,
  "peer_reviewed_likely": "yes",
  "keywords": [
    "1800 MHz",
    "radiofrequency",
    "mobile phone",
    "electromagnetic fields",
    "SAR 4 W/kg",
    "dendritic cells",
    "HLA-DR",
    "CD80",
    "CD86",
    "CD40",
    "CD11c",
    "allo-MLR",
    "IL-12p70",
    "TNF-alpha"
  ],
  "suggested_hubs": []
}
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AI-extracted fields are generated from the abstract and metadata and may be incomplete or incorrect. This material is for informational purposes and is not medical advice.

Identifiers and source

DOI: 10.3785/j.issn.1008-9292.2008.01.006
PubMed: 18275116

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