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Exposure to electromagnetic field attenuates oxygen-glucose deprivation-induced microglial cell death by reducing intracellular Ca(2+) and ROS.

International journal of radiation biology · 2016 · Benefit · Low evidence

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BenefitLow evidencePaper 4880

In HMO6 human microglial cells exposed to 4 h oxygen-glucose deprivation, EMF at 50 Hz/1 mT most strongly attenuated OGD-induced cell death and reduced OGD-associated increases in intracellular Ca(2+) and ROS. A smaller protective effect was observed at 10 Hz…

Study snapshot

PublicationInternational journal of radiation biology · 2016
Study typein_vitro
Reported directionBenefit
Evidence ratingLow

Abstract

Purpose The aim of this research was to demonstrate the protective effects of electromagnetic field (EMF) exposure on the human microglial cell line, HMO6, against ischemic cell death induced by in vitro oxygen-glucose deprivation (OGD). Materials and methods HMO6 cells were cultured for 4 h under OGD with or without exposure to EMF with different combinations of frequencies and intensities (10, 50, or 100 Hz/1 mT and 50 Hz/0.01, 0.1, or 1 mT). Cell survival, intracellular calcium and reactive oxygen species (ROS) levels were measured. Results OGD caused significant HMO6 cell death as well as elevation of intracellular Ca(2+) and ROS levels. Among different combinations of EMF frequencies and intensities, 50 Hz/1 mT EMF was the most potent to attenuate OGD-induced cell death and intracellular Ca(2+) and ROS levels. A significant but less potent protective effect was also found at 10 Hz/1 mT, whereas no protective effect was found at other combinations of EMF. A xanthine oxidase inhibitor reversed OGD-induced ROS production and cell death, while NADPH oxidase and mitochondrial respiration chain complex II inhibitors did not affect cell death. Conclusions 50 Hz/1 mT EMF protects human microglial cells from OGD-induced cell death by interfering with OGD-induced elevation of intracellular Ca(2+) and ROS levels, and xanthine oxidase is one of the main mediators involved in OGD-induced HMO6 cell death. Non-invasive treatment of EMF radiation may be clinically useful to attenuate hypoxic-ischemic brain injury.

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AI evidence extraction

Structured fields below were generated from the paper’s abstract and metadata to make the evidence easier to inspect. Automated extraction may be incomplete or incorrect; verify important details against the original paper.

Study typein_vitro
Effect directionbenefit
PopulationHuman microglial cell line (HMO6)
Sample sizeNot supplied
ExposureELF · 4 h (during OGD culture)
Evidence strengthLowConfidence: 86% · Peer reviewed: yes

Main findings

In HMO6 human microglial cells exposed to 4 h oxygen-glucose deprivation, EMF at 50 Hz/1 mT most strongly attenuated OGD-induced cell death and reduced OGD-associated increases in intracellular Ca(2+) and ROS. A smaller protective effect was observed at 10 Hz/1 mT, while other tested frequency/intensity combinations showed no protective effect.

Outcomes measured

  • Microglial cell survival/cell death under oxygen-glucose deprivation (OGD)
  • Intracellular Ca(2+) levels
  • Reactive oxygen species (ROS) levels

Limitations

  • In vitro study in a single human microglial cell line (HMO6)
  • Exposure conditions tested were limited to specific ELF frequencies (10–100 Hz) and magnetic flux densities (0.01–1 mT)
  • Sample size and replication details not provided in the abstract
  • Clinical usefulness is suggested but not tested in vivo or in humans
View raw extracted JSON
{
  "study_type": "in_vitro",
  "exposure": {
    "band": "ELF",
    "source": null,
    "frequency_mhz": null,
    "sar_wkg": null,
    "duration": "4 h (during OGD culture)"
  },
  "population": "Human microglial cell line (HMO6)",
  "sample_size": null,
  "outcomes": [
    "Microglial cell survival/cell death under oxygen-glucose deprivation (OGD)",
    "Intracellular Ca(2+) levels",
    "Reactive oxygen species (ROS) levels"
  ],
  "main_findings": "In HMO6 human microglial cells exposed to 4 h oxygen-glucose deprivation, EMF at 50 Hz/1 mT most strongly attenuated OGD-induced cell death and reduced OGD-associated increases in intracellular Ca(2+) and ROS. A smaller protective effect was observed at 10 Hz/1 mT, while other tested frequency/intensity combinations showed no protective effect.",
  "effect_direction": "benefit",
  "limitations": [
    "In vitro study in a single human microglial cell line (HMO6)",
    "Exposure conditions tested were limited to specific ELF frequencies (10–100 Hz) and magnetic flux densities (0.01–1 mT)",
    "Sample size and replication details not provided in the abstract",
    "Clinical usefulness is suggested but not tested in vivo or in humans"
  ],
  "evidence_strength": "low",
  "confidence": 0.86,
  "peer_reviewed_likely": "yes",
  "keywords": [
    "electromagnetic field",
    "ELF-EMF",
    "microglia",
    "HMO6",
    "oxygen-glucose deprivation",
    "ischemia",
    "cell death",
    "intracellular calcium",
    "reactive oxygen species",
    "xanthine oxidase",
    "50 Hz",
    "1 mT"
  ],
  "suggested_hubs": []
}
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AI-extracted fields are generated from the abstract and metadata and may be incomplete or incorrect. This material is for informational purposes and is not medical advice.

Identifiers and source

DOI: 10.3109/09553002.2016.1136851
PubMed: 26882219

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