RF Safe

RF-EMF exposure at 1800 MHz did not elicit DNA damage or abnormal cellular behaviors in different neurogenic cells.

Bioelectromagnetics · 2017 · No effect · Low evidence

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No effectLow evidencePaper 4810

Intermittent exposure to 1800 MHz RF-EMF at average SAR 4.0 W/kg for 1, 6, or 24 h did not significantly induce γH2AX foci in A172, U251, or SH-SY5Y cells and did not cause abnormal cell cycle progression, proliferation, or viability. Incubation up to 48 h af…

Study snapshot

PublicationBioelectromagnetics · 2017
Study typein_vitro
Reported directionNo effect
Evidence ratingLow

Abstract

Despite many years of studies, the debate on genotoxic effects of radiofrequency electromagnetic fields (RF-EMF) continues. To systematically evaluate genotoxicity of RF-EMF, this study examined effects of RF-EMF on DNA damage and cellular behavior in different neurogenic cells. Neurogenic A172, U251, and SH-SY5Y cells were intermittently (5 min on/10 min off) exposed to 1800 MHz RF-EMF at an average specific absorption rate (SAR) of 4.0 W/kg for 1, 6, or 24 h. DNA damage was evaluated by quantification of γH2AX foci, an early marker of DNA double-strand breaks. Cell cycle progression, cell proliferation, and cell viability were examined by flow cytometry, hemocytometer, and cell counting kit-8 assay, respectively. Results showed that exposure to RF-EMF at an SAR of 4.0 W/kg neither significantly induced γH2AX foci formation in A172, U251, or SH-SY5Y cells, nor resulted in abnormal cell cycle progression, cell proliferation, or cell viability. Furthermore, prolonged incubation of these cells for up to 48 h after exposure did not significantly affect cellular behavior. Our data suggest that 1800 MHz RF-EMF exposure at 4.0 W/kg is unlikely to elicit DNA damage or abnormal cellular behaviors in neurogenic cells. Bioelectromagnetics. 38:175-185, 2017. © 2016 Wiley Periodicals, Inc.

Machine Enhanced Logic

AI evidence extraction

Structured fields below were generated from the paper’s abstract and metadata to make the evidence easier to inspect. Automated extraction may be incomplete or incorrect; verify important details against the original paper.

Study typein_vitro
Effect directionno_effect
PopulationNeurogenic cell lines (A172, U251, SH-SY5Y)
Sample sizeNot supplied
ExposureRF · 1800 MHz · 4 W/kg · Intermittent (5 min on/10 min off) for 1, 6, or 24 h; follow-up incubation up to 48 h post-exposure
Evidence strengthLowConfidence: 86% · Peer reviewed: yes

Main findings

Intermittent exposure to 1800 MHz RF-EMF at average SAR 4.0 W/kg for 1, 6, or 24 h did not significantly induce γH2AX foci in A172, U251, or SH-SY5Y cells and did not cause abnormal cell cycle progression, proliferation, or viability. Incubation up to 48 h after exposure also did not significantly affect cellular behavior.

Outcomes measured

  • DNA damage (γH2AX foci; marker of DNA double-strand breaks)
  • Cell cycle progression
  • Cell proliferation
  • Cell viability
  • Cellular behavior up to 48 h post-exposure
View raw extracted JSON
{
  "study_type": "in_vitro",
  "exposure": {
    "band": "RF",
    "source": null,
    "frequency_mhz": 1800,
    "sar_wkg": 4,
    "duration": "Intermittent (5 min on/10 min off) for 1, 6, or 24 h; follow-up incubation up to 48 h post-exposure"
  },
  "population": "Neurogenic cell lines (A172, U251, SH-SY5Y)",
  "sample_size": null,
  "outcomes": [
    "DNA damage (γH2AX foci; marker of DNA double-strand breaks)",
    "Cell cycle progression",
    "Cell proliferation",
    "Cell viability",
    "Cellular behavior up to 48 h post-exposure"
  ],
  "main_findings": "Intermittent exposure to 1800 MHz RF-EMF at average SAR 4.0 W/kg for 1, 6, or 24 h did not significantly induce γH2AX foci in A172, U251, or SH-SY5Y cells and did not cause abnormal cell cycle progression, proliferation, or viability. Incubation up to 48 h after exposure also did not significantly affect cellular behavior.",
  "effect_direction": "no_effect",
  "limitations": [],
  "evidence_strength": "low",
  "confidence": 0.86,
  "peer_reviewed_likely": "yes",
  "keywords": [
    "RF-EMF",
    "1800 MHz",
    "SAR 4.0 W/kg",
    "genotoxicity",
    "DNA damage",
    "γH2AX",
    "double-strand breaks",
    "cell cycle",
    "cell proliferation",
    "cell viability",
    "neurogenic cells",
    "A172",
    "U251",
    "SH-SY5Y"
  ],
  "suggested_hubs": []
}
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AI-extracted fields are generated from the abstract and metadata and may be incomplete or incorrect. This material is for informational purposes and is not medical advice.

Identifiers and source

DOI: 10.1002/bem.22032
PubMed: 28026047

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