Inhibition of mitochondrial calcium uptake by Ru360 enhances the effect of 1800 MHz radio-frequency electromagnetic fields on DNA damage

2023 · Mixed · Low evidence

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MixedLow evidencePaper 375

Short-time (15 min) exposure to 1800 MHz RF-EMF induced significant DNA damage and cell apoptosis in mouse embryonic fibroblasts treated with Ru360 (an MCU inhibitor). No significant effects were observed on cell cycle, cell proliferation, or cell viability; …

Study snapshot

Publicationmanual · 2023

Study typein_vitro

Reported directionMixed

Evidence ratingLow

Abstract

Inhibition of mitochondrial calcium uptake by Ru360 enhances the effect of 1800 MHz radio-frequency electromagnetic fields on DNA damage Sun C, Zhu L, Qin H, Su H, Zhang J, Wang S, Xu X, Zhao Z, Mao G, Chen J. Inhibition of mitochondrial calcium uptake by Ru360 enhances the effect of 1800 MHz radio-frequency electromagnetic fields on DNA damage. Ecotoxicol Environ Saf. 2023 Sep 14;264:115472. doi: 10.1016/j.ecoenv.2023.115472. Highlights • Ru360, a specific inhibitor of MCU, enhanced the DNA damage effect of short-time (15 min) exposure to1800 MHz RF-EMF • ATP synthesis play a role in cellular resistance to 1800 MHz RF-EMF short-time exposure on DNA damage. • DNA damage induced by 1800 MHz RF-EMF in Ru360 treated cells were quickly repaired within 1 h after exposure. Abstract Today, the existence of radio-frequency electromagnetic fields (RF-EMF) emitted from cell phones, wireless routers, base stations, and other sources are everywhere around our living environment, and the dose is increasing. RF-EMF have been reported to be cytotoxic and supposed to be a risk factor for various human diseases, thus, more attention is necessary. In recent years, interfere with mitochondrial calcium uptake by using mitochondrial calcium uniporter (MCU) inhibitor were suggested to be potential clinical treatment in mitochondrial calcium overload diseases, like neurodegeneration, ischemia/reperfusion injury, and cancer, but whether this approach increases the health risk of RF-EMF exposure are unknown. To address our concern, we did a preliminary study to determine whether inhibition of MCU will increase the genotoxicity of RF-EMF exposure in cells, and found that short-time (15 min) exposure to 1800 MHz RF-EMF induced significant DNA damage and cell apoptosis in mouse embryonic fibroblasts (MEFs) treated with Ruthenium 360 (Ru360), a specific inhibitor of MCU, but no significant effects on cell cycle, cell proliferation, or cell viability were observed. In conclusion, our results indicated that inhibiting MCU increases the genotoxicity of RF-EMF exposure, and more attention needs to be paid to the possible health impact of RF-EMF exposure under these treatments. Open access paper: sciencedirect.com

Machine Enhanced Logic

AI evidence extraction

Structured fields below were generated from the paper’s abstract and metadata to make the evidence easier to inspect. Automated extraction may be incomplete or incorrect; verify important details against the original paper.

Study typein_vitro

Effect directionmixed

PopulationMouse embryonic fibroblasts (MEFs)

Sample sizeNot supplied

ExposureRF · 1800 MHz · 15 min

Evidence strengthLowConfidence: 78% · Peer reviewed: yes

Main findings

Outcomes measured

DNA damage
Cell apoptosis
Cell cycle
Cell proliferation
Cell viability
ATP synthesis (role in resistance; mentioned in highlights)
DNA repair within 1 h after exposure (mentioned in highlights)

Limitations

Preliminary study (as stated by authors)
In vitro cell model (MEFs); no human/animal outcomes reported
Exposure metric details (e.g., SAR/dose) not provided in abstract

View raw extracted JSON

{
  "study_type": "in_vitro",
  "exposure": {
    "band": "RF",
    "source": null,
    "frequency_mhz": 1800,
    "sar_wkg": null,
    "duration": "15 min"
  },
  "population": "Mouse embryonic fibroblasts (MEFs)",
  "sample_size": null,
  "outcomes": [
    "DNA damage",
    "Cell apoptosis",
    "Cell cycle",
    "Cell proliferation",
    "Cell viability",
    "ATP synthesis (role in resistance; mentioned in highlights)",
    "DNA repair within 1 h after exposure (mentioned in highlights)"
  ],
  "main_findings": "Short-time (15 min) exposure to 1800 MHz RF-EMF induced significant DNA damage and cell apoptosis in mouse embryonic fibroblasts treated with Ru360 (an MCU inhibitor). No significant effects were observed on cell cycle, cell proliferation, or cell viability; DNA damage in Ru360-treated cells was reported to be quickly repaired within 1 h after exposure (highlight).",
  "effect_direction": "mixed",
  "limitations": [
    "Preliminary study (as stated by authors)",
    "In vitro cell model (MEFs); no human/animal outcomes reported",
    "Exposure metric details (e.g., SAR/dose) not provided in abstract"
  ],
  "evidence_strength": "low",
  "confidence": 0.78,
  "peer_reviewed_likely": "yes",
  "keywords": [
    "1800 MHz",
    "RF-EMF",
    "radio-frequency electromagnetic fields",
    "DNA damage",
    "genotoxicity",
    "mitochondrial calcium uniporter",
    "MCU",
    "Ru360",
    "mouse embryonic fibroblasts",
    "apoptosis"
  ],
  "suggested_hubs": [
    {
      "slug": "who-icnirp",
      "weight": 0.2,
      "reason": "General RF-EMF health risk context mentioned; no specific policy evaluation in abstract."
    }
  ]
}

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AI-extracted fields are generated from the abstract and metadata and may be incomplete or incorrect. This material is for informational purposes and is not medical advice.

Identifiers and source

DOI: 10.1016/j.ecoenv.2023.115472
PubMed: Not supplied

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