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Exposure to an extremely low-frequency electromagnetic field only slightly modifies the proteome of Chromobacterium violaceumATCC 12472.

Genetics and molecular biology · 2015 · Mixed · Low evidence

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MixedLow evidencePaper 4759

In situ exposure to ELF-EMF produced minimal effects on the global protein expression profile of Chromobacterium violaceum. The proteome was only slightly altered, with five differentially expressed proteins detected; two (DNA-binding stress protein Dps and a…

Study snapshot

PublicationGenetics and molecular biology · 2015
Study typein_vitro
Reported directionMixed
Evidence ratingLow

Abstract

Several studies of the physiological responses of different organisms exposed to extremely low-frequency electromagnetic fields (ELF-EMF) have been described. In this work, we report the minimal effects of in situ exposure to ELF-EMF on the global protein expression of Chromobacterium violaceum using a gel-based proteomic approach. The protein expression profile was only slightly altered, with five differentially expressed proteins detected in the exposed cultures; two of these proteins (DNA-binding stress protein, Dps, and alcohol dehydrogenase) were identified by MS/MS. The enhanced expression of Dps possibly helped to prevent physical damage to DNA. Although small, the changes in protein expression observed here were probably beneficial in helping the bacteria to adapt to the stress generated by the electromagnetic field.

Machine Enhanced Logic

AI evidence extraction

Structured fields below were generated from the paper’s abstract and metadata to make the evidence easier to inspect. Automated extraction may be incomplete or incorrect; verify important details against the original paper.

Study typein_vitro
Effect directionmixed
PopulationChromobacterium violaceum ATCC 12472 (bacterial cultures)
Sample sizeNot supplied
ExposureELF
Evidence strengthLowConfidence: 74% · Peer reviewed: yes

Main findings

In situ exposure to ELF-EMF produced minimal effects on the global protein expression profile of Chromobacterium violaceum. The proteome was only slightly altered, with five differentially expressed proteins detected; two (DNA-binding stress protein Dps and alcohol dehydrogenase) were identified by MS/MS.

Outcomes measured

  • Global protein expression/proteome changes
  • Differentially expressed proteins (gel-based proteomics)
  • Identification of proteins by MS/MS (Dps, alcohol dehydrogenase)

Limitations

  • Exposure parameters (e.g., frequency, field strength, duration) not reported in the abstract
  • Only two of five differentially expressed proteins were identified by MS/MS (per abstract)
  • Proteomic approach described as gel-based; broader coverage/validation not described in the abstract
View raw extracted JSON
{
  "study_type": "in_vitro",
  "exposure": {
    "band": "ELF",
    "source": null,
    "frequency_mhz": null,
    "sar_wkg": null,
    "duration": null
  },
  "population": "Chromobacterium violaceum ATCC 12472 (bacterial cultures)",
  "sample_size": null,
  "outcomes": [
    "Global protein expression/proteome changes",
    "Differentially expressed proteins (gel-based proteomics)",
    "Identification of proteins by MS/MS (Dps, alcohol dehydrogenase)"
  ],
  "main_findings": "In situ exposure to ELF-EMF produced minimal effects on the global protein expression profile of Chromobacterium violaceum. The proteome was only slightly altered, with five differentially expressed proteins detected; two (DNA-binding stress protein Dps and alcohol dehydrogenase) were identified by MS/MS.",
  "effect_direction": "mixed",
  "limitations": [
    "Exposure parameters (e.g., frequency, field strength, duration) not reported in the abstract",
    "Only two of five differentially expressed proteins were identified by MS/MS (per abstract)",
    "Proteomic approach described as gel-based; broader coverage/validation not described in the abstract"
  ],
  "evidence_strength": "low",
  "confidence": 0.74,
  "peer_reviewed_likely": "yes",
  "keywords": [
    "extremely low-frequency electromagnetic field",
    "ELF-EMF",
    "proteomics",
    "Chromobacterium violaceum",
    "differential protein expression",
    "Dps",
    "alcohol dehydrogenase",
    "MS/MS"
  ],
  "suggested_hubs": []
}
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AI-extracted fields are generated from the abstract and metadata and may be incomplete or incorrect. This material is for informational purposes and is not medical advice.

Identifiers and source

DOI: 10.1590/s1415-4757382220140240
PubMed: 26273227

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